酸化還元特性を持つ膜小胞によるバイオフィルム活性の制御

通过具有氧化还原特性的膜囊泡控制生物膜活性

• 批准号: 19F19406
• 负责人: 岡本 章玄
• 金额: $ 1.47万
• 依托单位: National Institute for Materials Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-11-08 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19406/
• 关键词: Nitrogen fixing; Multi-heme Cytochrome C; Exciton coupling; Circular dichroism; バイオフィルム; 電気化学; 二次イオン質量分析; 電気細菌; 膜小胞; Nitrogen fixing; Multi-heme Cytochrome C; Exciton coupling; Circular dichroism; バイオフィルム; 電気化学; 二次イオン質量分析; 電気細菌; 膜小胞

The electrochemical reactor systems currently used for current production and drug testing involve relatively large volumes, making these systems infeasible for high-throughput screening of potential antimicrobials. We used screen-printed electrodes or multi-well electrode systems that require minimum amounts of sample and antimicrobials. Such systems that exploit the electrogenic activity of pathogens could fast-track the discovery of antibiofilm drugs, as a large library of compounds could be rapidly screened. This concept could serve as a generally applicable technology for evaluating the efficacy of antimicrobials, as well as the selection of appropriate drugs or treatment regimens. Our works extended the boundaries of EET field to other niches such as human pathogens. The orthologues of the identified EET genes in human pathogens are present in hundreds of species, thus EET activity likely occurred in an evolutionarily diverse subset of bacteria. Consequently, our research can help screen the effects of antimicrobials on biofilm activity by employing the current producing capability in wide range of electroactive pathogens.

当前用于当前生产和药物测试的电化学反应器系统涉及相对较大的体积，这使得这些系统不可避免地用于对潜在抗菌剂的高通量筛查。我们使用了需要最少样品和抗菌剂的屏幕打印电极或多孔电极系统。这种利用病原体的电源活性的系统可以快速筛选出大型化合物库，从而快速追踪抗生素药物的发现。该概念可以用作评估抗菌药物的功效的通常适用技术，以及选择适当的药物或治疗方案的方法。我们的作品将EET领域的边界扩展到其他壁ni，例如人类病原体。人类病原体中鉴定出的EET基因的直系同源物存在于数百种物种中，因此EET活性可能发生在细菌的进化多种子集中。因此，我们的研究可以通过在广泛的电活性病原体中采用当前产生能力来帮助筛选抗菌素对生物膜活性的影响。