Multidimensional fast gas chromatography on basis of flow-field thermal gradient gas chromatography

基于流场热梯度气相色谱的多维快速气相色谱

基本信息

批准号：
326648191
负责人：
Privatdozent Dr. Peter Boeker
金额：
--
依托单位：
Institut für Landtechnik (ILT)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2020-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/326648191?language=en
关键词：
Multidimensional fast gas chromatography basis

项目摘要

The project within the framework of the program New devices for research is aimed at the development of a new type of fast multidimensional gas chromatography. Currently the measurements are made with complex measurement systems whose data are and difficult to interpret. This prevents a broad adoption of this technology in basic research. The new gas chromatographic method will be fast and easy to use. In different areas of basic research a more differentiated look on chemical complexity will be established. Furthermore, dynamic processes can be observed by the fast cycling measurement system. New research over extensive sample numbers becomes possible. In classical gas chromatography co-elution is frequent, especially in fast and low resolution measurements. Two dimensional gas chromatography suppresses co-elution with two successive columns and two different separation mechanisms. Two different methods are established. With heart-cutting GC selected time intervals from the first dimension separation are cut to the second dimension column. In comprehensive GC, or GCxGC, the whole first dimension separation is modulated in very short time intervals and separated on a short and fast second column. The proposal describes an alternative approach which has some advantages compared to the present state-of-the-art. It is based on a new basic technology, the flow-field thermal gradient gas chromatography. This separation method yields high resolution in short cycles even below one minute. The thermal gradient focuses the signals, the modified chromatographic transport lowers elution temperatures substantially. Therefore the application range of GC is expanded. The conceptual and technical simplicity makes FF-TG-GC an ideal module for multidimensional gas chromatographic set-ups. The basic idea is the coupling of two FF-TG-GC systems. The sample mixtures are separated on a short first column in an intermittent way. This has become possible with the very fast cooling flow-field principle. By valveless switching (e.g. a Deans switch) the eluates are directed to the second column. Here, complete temperature and gradient programmed separation on an orthogonal column takes place in the thermal gradient mode.

该计划的框架内的项目新研究设备旨在开发一种新型的快速多维气相色谱法。当前，测量是通过数据且难以解释的复杂测量系统进行的。这防止了这项技术在基础研究中的广泛采用。新的气相色谱法将快速易于使用。在基础研究的不同领域，将建立对化学复杂性的更具不同的看法。此外，可以通过快速循环测量系统观察到动态过程。对大量样本数量进行的新研究是可能的。在经典气相色谱法中，共同布置频繁，尤其是在快速和低分辨率测量中。二维气相色谱法抑制了两个连续的柱和两个不同的分离机制的共同精心统治。建立了两种不同的方法。通过切开心脏的GC选择，从第一个维度分离选择的时间间隔被切为第二维列。在综合的GC或GCXGC中，整个第一维分隔在很短的时间间隔中进行调制，并在短而快速的第二列中分开。该提案描述了一种替代方法，该方法与目前的最新方法相比具有一些优势。它基于一种新的基本技术，即流场热梯度气相色谱法。这种分离方法甚至在一分钟以下的短周期中产生高分辨率。热梯度聚焦信号，修饰的色谱传输大大降低了洗脱温度。因此，GC的应用范围扩展。概念和技术简单性使FF-TG-GC成为多维气体色谱设置的理想模块。基本思想是两个FF-TG-GC系统的耦合。样品混合物以间歇性的方式在简短的第一列中分开。通过非常快速的冷却流场原理，这可能是可能的。通过Valveless开关（例如，Deans Switch），将脱位定向到第二列。在这里，在正交柱上的完整温度和梯度编程的分离发生在热梯度模式下。