Testing recurrent ecological speciation processes in microendemic Madagascan reptiles and amphibians of the isolated Amber Mountain

测试孤立琥珀山微流行马达加斯加爬行动物和两栖动物的循环生态物种形成过程

基本信息

批准号：
322418141
负责人：
Professor Dr. Miguel Vences
金额：
--
依托单位：
Zoologisches Institut
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2016
资助国家：
德国
起止时间：
2015-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/322418141?language=en
关键词：
Testing recurrent ecological speciation processes

项目摘要

The processes by which new species arise have long been an active field of debate Current research on speciation focuses the mechanisms driving the divergence. Ecological Speciation (ES) occurs if natural selection strongly favours different ecotypes, and reproductive isolation evolves as a consequence of adaptive divergence due to differing ecology. However, suitable settings to study ES are rare. Our long-term research program on the evolution of the vertebrate fauna of Madagascar has yielded preliminary indicated that the isolated Amber Mountain Massif in northern Madagascar harbours distinct locally endemic sister clades of various amphibians and reptiles, and some of these appear to be restricted to distinct elevational ranges. This suggests that these lineages might have originated in the Massif by ES, and points to Amber Mountain as a suitable model system for studying diversification across ecological (elevational) gradients. The research project proposed here will combine cutting-edge methods in metabarcoding and population genetics, to explore ES processes in the microendemic herpetofauna of this massif. It applies a strict hypothesis-testing framework to assess whether an ecological gradient is the predominant driver of past and ongoing ES. Our approach will use metabarcoding data for the entire herpetofaunal community of Amber Mountain, and population genetic in-depth study of three target taxa (likely including one or several of the genera Brookesia, Stumpffia, Phelsuma, or Uroplatus). Dense sampling will be carried out along pre-defined transects that will be characterized ecologically, and furthermore, rapid surveys will be done using a grid approach in order to characterize the distribution and phylogeography of the entire herpetofauna in the massif. The large number of samples collected during fieldwork will be analyzed using high-throughput amplicon metabarcoding of pooled samples with Illumina sequencing. For the target taxa, microsatellites and RADseq-derived SNPs will be used to characterize small-scale population differentiation. If ES triggered the diversification of amphibians and reptiles of Amber Mountain, then we predict that (i) endemic sister lineages that differentiated on Amber Mountain will be distributed at distinct elevational ranges, (ii) their contact zones coincide with ecotones and with turnover of the entire herpetofaunal community, (iii) target lineages distributed across these ecotones will show reduction of gene flow related to the ecological gradient rather than to distance or physical barriers. Given the scarcity of research that tests ES in Madagascar, the endeavour to establish the Amber Mountain as study region for such research would be ground-breaking, and stimulate process-based speciation research in this unique biota.

新物种产生的过程长期以来一直是一个活跃的争论领域，当前对物种形成的研究重点是驱动物种分化的机制。如果自然选择强烈偏爱不同的生态类型，就会发生生态物种形成（ES），并且由于不同的生态而产生适应性分歧，从而导致生殖隔离的进化。然而，适合研究 ES 的环境很少。我们对马达加斯加脊椎动物区系进化的长期研究计划已初步表明，马达加斯加北部孤立的琥珀山地块蕴藏着各种两栖动物和爬行动物的独特当地特有姐妹进化枝，其中一些似乎仅限于不同的物种。海拔范围。这表明这些谱系可能起源于 ES 的高原，并指出琥珀山是研究生态（海拔）梯度多样化的合适模型系统。这里提出的研究项目将结合元条形码和群体遗传学的尖端方法，探索该地块微型地方性爬行动物的 ES 过程。它采用严格的假设检验框架来评估生态梯度是否是过去和正在进行的生态系统的主要驱动因素。我们的方法将使用琥珀山整个爬行动物群落的元条形码数据，并对三个目标类群（可能包括 Brookesia、Stummpffia、Phelsuma 或 Uroplatus 属中的一种或几种）进行群体遗传学深入研究。将沿着预先定义的横断面进行密集采样，这些横断面将进行生态表征，此外，将使用网格方法进行快速调查，以表征地块中整个爬行动物的分布和系统发育特征。现场工作期间收集的大量样本将使用 Illumina 测序对合并样本进行高通量扩增子元条形码进行分析。对于目标分类单元，微卫星和 RADseq 衍生的 SNP 将用于表征小规模种群分化。如果ES引发了琥珀山两栖动物和爬行动物的多样化，那么我们预测：（i）在琥珀山分化的特有姐妹谱系将分布在不同的海拔范围内，（ii）它们的接触带与生态交错带和生态交错带的更替一致。整个爬行动物群落，（iii）分布在这些生态交错带的目标谱系将显示与生态梯度有关的基因流减少，而不是与距离或物理障碍有关。鉴于马达加斯加缺乏测试 ES 的研究，将琥珀山建立为此类研究的研究区域的努力将是开创性的，并将刺激这个独特生物群中基于过程的物种形成研究。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Computational molecular species delimitation and taxonomic revision of the gecko genus Ebenavia Boettger, 1878

壁虎属 Ebenavia Boettger 的计算分子物种界定和分类学修订，1878

DOI：
10.1007/s00114-018-1574-9
发表时间：
2018-07-20
期刊：
The Science of Nature
影响因子：
0
作者：
O. Hawlitschek;M. Scherz;B. Ruthensteiner;A. Crottini;F. Glaw
通讯作者：
F. Glaw

Sympatric lineages in the Mantidactylus ambreensis complex of Malagasy frogs originated allopatrically rather than by in-situ speciation.

马达加斯加青蛙的Mantidactylus ambreensis复合体中的同域谱系起源于异域分布，而不是原位物种形成。

DOI：
10.1016/j.ympev.2019.106700
发表时间：
2019-12-03
期刊：
Molecular phylogenetics and evolution
影响因子：
4.1
作者：
Safidy M Rasolonjatovo;M. Scherz;C. Hutter;F. Glaw;Andolalao Rakotoarison;Jary H. Razafindraibe;S. Goodman
通讯作者：
S. Goodman

Two new Pandanus frogs (Guibemantis: Mantellidae: Anura) from northern Madagascar

来自马达加斯加北部的两只新露兜树蛙（Guibemantis：Mantellidae：Anura）