During a protein spray drying process, thermal and mechanical stresses are exerted on the proteins leading to degradation. We hypothesize that in situ measurements of the droplet size, temperature distribution inside droplets and UV fluorescence will prov

在蛋白质喷雾干燥过程中，热应力和机械应力作用于蛋白质，导致蛋白质降解。

基本信息

批准号：
315006086
负责人：
Professor Dr.-Ing. Lutz Mädler
金额：
--
依托单位：
Fachgebiet Mechanische Verfahrenstechnik
依托单位国家：
德国
项目类别：
Priority Programmes
财政年份：
2016
资助国家：
德国
起止时间：
2015-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/315006086?language=en
关键词：
During protein spray drying process

项目摘要

Protein processing and formulating often involves the removal of excess water. Spray drying is one of the most widely used unit operations for the production of stable protein containing powders. In spray drying a stock solution is atomized while subsequent evaporation increases the protein concentration within the evaporating droplets. During this drying process, thermal and mechanical stresses are exerted on the proteins that can result into degradation. Depending on the polar groups of the proteins they will accumulate at the air-water interface, forming a surface layer of proteins oriented in such a way that their hydrophilic parts are exposed to water which can cause unfolding.A fundamental understanding of the mechanisms of protein degradation is important for designing a spray drying process. The kinetics and interactions of such thermal and mechanical stresses on proteins are, however, not well understood at the single droplet level. There is no study on the time resolved kinetics of the denaturation processes at the droplet level.Experiments with single droplets are a common technique to investigate the basics of spray drying. Single droplets experiments will be conducted using a piezoelectric droplet generator in a drying chamber. The droplet size will be monitored with a camera and Mie scattering imaging. This method also gives information on the formation of aggregates and precipitation within a droplet. Temperature and protein concentration profiles will be measured with the Rainbow technique. We will use fluorescence spectroscopy with specific fluoresce dyes to monitor the unfolding and denaturation of the proteins.Our objective is a relationship between spray drying process parameters such as, droplet size, protein concentration and protein functional groups, solution additives as well as gas composition and temperature and the degradation kinetics. We hypothesize that time resolved, in situ measurements of the droplet size, temperature distribution inside droplets and protein UV fluorescence will provide degradation kinetics.

蛋白质加工和配方通常涉及去除多余的水。喷雾干燥是用于生产含有粉末的稳定蛋白质的最广泛使用的单元操作之一。在喷雾干燥时，储备溶液被雾化，而随后的蒸发增加了蒸发液滴内的蛋白质浓度。在此干燥过程中，在蛋白质上施加热应力和机械应力，从而导致降解。根据它们将在空气水接口上积聚的蛋白质的极性基团，形成了定向蛋白质的表面层，以使其亲水性部分暴露于水中，这可能会导致展开。对蛋白质脱落机制的基本了解对于设计喷雾干燥过程至关重要。然而，这种热和机械应力在蛋白质上的动力学和相互作用在单滴水水平上尚未得到很好的了解。尚无研究在液滴级别上解决变性过程的时间动力学。单滴的经验是研究喷雾干燥的基础知识的常见技术。将使用干燥室中的压电液滴发生器进行单液滴实验。液滴尺寸将通过相机和MIE散射成像进行监视。该方法还提供了有关液滴内聚集体和降水的信息。温度和蛋白质浓度轮廓将通过彩虹技术测量。我们将使用与特定荧光染料的荧光光谱法监测蛋白质的展开和变性。我们的目标是喷雾干燥过程参数（例如，液滴大小，蛋白质浓度和蛋白质官能团），溶液添加剂，气体组成和温度以及脱脂力动力学的关系。我们假设时间已经解决，原位测量了液滴尺寸，液滴内部的温度分布和蛋白UV荧光将提供降解动力学。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Rainbow pattern analysis of a multilayered sphere for optical diagnostic of a heating droplet

DOI：
10.1016/j.optcom.2019.02.061
发表时间：
2019-06
期刊：
Optics Communications
影响因子：
2.4
作者：
Jianxi Zhou;Yuan Fang;Jiajie Wang;Le Zhu;T. Wriedt
通讯作者：
Jianxi Zhou;Yuan Fang;Jiajie Wang;Le Zhu;T. Wriedt

Internal field distribution of a radially inhomogeneous droplet illuminated by an arbitrary shaped beam

任意形状光束照射的径向不均匀液滴的内部场分布

DOI：
10.1016/j.jqsrt.2018.02.012
发表时间：
2018
期刊：
Journal of Quantitative Spectroscopy and Radiative Transfer
影响因子：
2.3
作者：
Wang Jia Jie;Wriedt Thomas;Han Yi Ping;Maedler Lutz;Jiao Yong Chang
通讯作者：
Jiao Yong Chang

Multipole expansion of circularly symmetric Bessel beams of arbitrary order for scattering calculations

用于散射计算的任意阶圆对称贝塞尔光束的多极展开

DOI：
10.1016/j.optcom.2016.11.038
发表时间：
2017-03
期刊：
Optics Communications
影响因子：
2.4
作者：
Wang Jia Jie;Wriedt Thomas;Maedler Lutz;Han Yi Ping;Hartmann Peter
通讯作者：
Hartmann Peter

General description of transverse mode Bessel beams and construction of basis Bessel fields

横模贝塞尔梁的一般描述和基础贝塞尔场的构造

DOI：
10.1016/j.jqsrt.2016.10.010
发表时间：
2017-07
期刊：
Journal of Quantitative Spectroscopy and Radiative Transfer
影响因子：
2.3
作者：
Wang Jia Jie;Wriedt Thomas;Lock James A.;Jiao Yong Chang
通讯作者：
Jiao Yong Chang

Simultaneous measurement of monocomponent droplet temperature/refractive index, size and evaporation rate with phase rainbow refractometry

DOI：
10.1016/j.jqsrt.2018.04.034
发表时间：
2018-05
期刊：
Journal of Quantitative Spectroscopy and Radiative Transfer
影响因子：
2.3
作者：
Yingchun Wu;C. Crua;Haipeng Li;S. Saengkaew;L. Mädler;Xue-cheng Wu;G. Grehan
通讯作者：
Yingchun Wu;C. Crua;Haipeng Li;S. Saengkaew;L. Mädler;Xue-cheng Wu;G. Grehan