Molecular mapping of lipid biomarkers with Time-of-Flight - Secondary Ion Mass Spectrometry (TOF-SIMS): a feasibility study.

使用飞行时间-二次离子质谱 (TOF-SIMS) 进行脂质生物标志物的分子图谱：可行性研究。

• 批准号: 31230282
• 负责人: Professor Dr. Volker Thiel
• 金额: --
• 依托单位: Abteilung Geobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/31230282?language=en
• 关键词: Molecular; mapping; lipid; biomarkers; Time

Time of Flight - Secondary Ion Mass Spectrometry (TOF-SIMS) has become a routine analytical tool in material research where it is mainly used for the characterisation of surface properties and composition. Specifically with the development of new polyatomic primary ion sources, TOF-SIMS can be used to perform high-quality chemical imaging by rastering over the sample surface and quasicontinuously collecting mass spectra. A molecular map of a sample area can thus be generated for any compound that produces a diagnostic mass peak in the spectrum. Although these advantageous features exactly meet the demands of many modern geobiological approaches, the potential of TOF-SIMS for such applications remains yet to be explored. In this project, we will systematically evaluate the capabilities and limits of TOF-SIMS in order to open a new analytical window in organic biomarker studies. Emphasis is placed on (1) the generation and interpretation of basic spectral reference data for major biolipids and geolipids, and (2) the identification and molecular mapping of these biomarkers in geobiological materials ranging from microbial mats to solid rocks. This project is designed as a feasibility study that will form the basis for a geobiological follow-on project on the sources and distributions of organic molecules in active microbial systems, and in related minerals and rocks.

飞行时间-二次离子质谱（TOF-SIMS）已成为材料研究中的常规分析工具，主要用于表征表面性质和成分。具体来说，随着新型多原子一次离子源的开发，TOF-SIMS 可用于通过在样品表面进行光栅扫描并准连续收集质谱来执行高质量化学成像。因此，可以为在光谱中产生诊断质量峰的任何化合物生成样品区域的分子图。尽管这些优势特征完全满足了许多现代地球生物学方法的需求，但 TOF-SIMS 在此类应用中的潜力仍有待探索。在这个项目中，我们将系统地评估TOF-SIMS的能力和局限性，以在有机生物标志物研究中打开一个新的分析窗口。重点放在（1）主要生物脂和地球脂的基本光谱参考数据的生成和解释，以及（2）从微生物席到固体岩石的地球生物材料中这些生物标志物的识别和分子图谱。该项目旨在作为一项可行性研究，为后续地球生物学项目奠定基础，研究活性微生物系统以及相关矿物和岩石中有机分子的来源和分布。