Out-of-Equilibrium Sensing with Unselective Artificial Receptors

非选择性人工受体的失平衡传感

• 批准号: 287182843
• 负责人: Dr. Frank Biedermann
• 金额: --
• 依托单位: Institut für Nanotechnologie (INT)
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/287182843?language=en
• 关键词: Out; Equilibrium; Sensing; Unselective; Artificial

An out-of-equilibrium sensing methodology utilizing non-selective artificial receptors will be developed that allows for the detection of biologically relevant analytes in aqueous media. First, it is shown that one can identify and quantify analytes by mere use of one non-selective receptor, which at first glance must appear highly unlikely to the reader. Our method can succeed because formerly unavailable analyte-related information can now be obtained using our novel out-of-equilibrium method that is coupled with a displacement or simultaneous-binding assay. In a complete contrast to established equilibrium-based assays with highly selective nature-derived receptors, this proposal enables for the first time the use of low-cost and robust but unselective artificial receptors for sensing under practical relevant circumstances. The concept-tested and patented out-of-equilibrium approach will be developed with the help of this research proposal a general sensing method (WP1) that is applicable for high-throughput screening (WP2). Furthermore, a kinetic array-based sensing method suitable for the detection and quantification of analytes in complex mixtures will be established (WP3).

将开发出一种利用非选择性人工受体的平衡传感方法，该方法可以在水性培养基中检测生物学相关的分析物。首先，结果表明，只需使用一个非选择性受体，就可以识别和量化分析物，乍看之下必须对读者看来很不可能。我们的方法可以成功，因为现在可以使用我们新颖的平衡方法与位移或同时结合测定法相结合的新颖平衡方法来获得以前与分析物相关的信息。与具有高度选择性自然衍生的受体的建立基于平衡的测定的完全鲜明对比，该提案首次可以在实际相关情况下首次使用低成本和健壮但不可选择的人工受体来感测。概念测试和专利的非平衡方法将在本研究建议的帮助下开发一种通用感应方法（WP1），适用于高通量筛选（WP2）。此外，将建立一种基于动力学阵列的传感方法，适合于检测和定量复杂混合物中的分析物（WP3）。