High-resolution low-voltage TEM for imaging the process of mineralization at the TMV/inorganic interface: Towards understanding the mechanical properties of bio/inorganic multilayer systems

用于对 TMV/无机界面矿化过程进行成像的高分辨率低压 TEM:了解生物/无机多层系统的机械性能

基本信息

项目摘要

Biomineralization is the natural process of construction and induration of tissues, which outmatch several synthesized products in their physical and chemical properties. Appreciably, one tries to synthesize comparable technical relevant hybrid materials by bio-inspired mineralization. In the existing project we would like to contribute to a better understanding of the mechanism of protein based mineralization of zinc sulfide (ZnS) and zinc oxide (ZnO) on the biotemplate tobacco mosaic virus (TMV) by imaging the organic/inorganic glue line with high-resolution. The obtained insights will help to control the structure of this boundary surface, which are essential for synthesis of nano-sized functional material with tailored properties.Toward the commonly used techniques to image organic materials by negative staining of the specimen, which leads to resolutions in the range of several nanometer or embedding them in vitrified ice with a resolution up to 0.4 nm at acceleration voltages of 300 kV, we count on our newly developed low-voltage transmission electron microscope (TEM) available in spring 2016 to image the surface boundary. High resolution images of the glue line at acceleration voltages of 20-80 kV is possible due to not only spherical but also chromatic aberration correction of the objective lens. In the frame of this project elaborate sample preparation methods like graphene-sandwiching and/or deuterating the specimen will be investigated to image the organic/inorganic boundary surface. Additionally, a further goal of this project is to reveal in situ if the bio-inspired mineralization begins with heterogeneous or homogenous nucleation.In preliminary work of the Stuttgart group, mineralization procedures for selective deposition of ZnS and ZnO on tobacco mosaic virus (TMV) were accomplished. In the frame of this project multilayered nacre-like structures (MLS) on the base of TMV/ZnS and TMV/ZnO, respectively will be produced and investigated. The mechanical properties investigations of ZnO-based MLS with wildtype (wt) TMV (wt-TMV/ZnO) and the TMV mutant TMV_Cys (TMV_Cys/ZnO) and therefore with additional thiols in the boundary surface revealed that the MLS containing the mutant showed an increase in hardness compared to wt-based one. Those MLS will also be imaged with our low-voltage TEM to analyze ultrastructural disparity of the protein-mineral-boundary surface and to correlate that with mechanical properties. To gain an insight with the TEM into the mineralization at the surface as well as in the inner channel of the virus it is additionally planned to assemble small virus-like particles (VLP) and mineralized them in vertical oriented arrays.
生物矿化是组织构建和硬化的自然过程,其物理和化学性质优于多种合成产品。值得注意的是,人们试图通过仿生矿化来合成类似技术相关的混合材料。在现有项目中,我们希望通过对有机/无机胶层进行成像,有助于更好地理解生物模板烟草花叶病毒 (TMV) 上硫化锌 (ZnS) 和氧化锌 (ZnO) 的蛋白质矿化机制。高分辨率。所获得的见解将有助于控制该边界表面的结构,这对于合成具有定制特性的纳米级功能材料至关重要。通过对样品进行负染色来对有机材料进行成像的常用技术,这导致了或将它们嵌入到分辨率高达 0.4 nm、加速电压为 300 kV 的玻璃化冰中,我们依靠 2016 年春季推出的新开发的低压透射电子显微镜 (TEM) 来成像表面边界。由于物镜的球面像差校正和色差校正,可以在 20-80 kV 的加速电压下获得高分辨率的胶层图像。在该项目的框架中,将研究详细的样品制备方法,例如石墨烯夹心和/或氘化样品,以对有机/无机边界表面进行成像。此外,该项目的另一个目标是原位揭示仿生矿化是否以异质或同质成核开始。在斯图加特小组的初步工作中,在烟草花叶病毒(TMV)上选择性沉积 ZnS 和 ZnO 的矿化程序都完成了。在该项目的框架内,将分别生产和研究基于 TMV/ZnS 和 TMV/ZnO 的多层珍珠质结构 (MLS)。对具有野生型 (wt) TMV (wt-TMV/ZnO) 和 TMV 突变体 TMV_Cys (TMV_Cys/ZnO) 以及边界表面中额外硫醇的 ZnO 基 MLS 的机械性能研究表明,含有突变体的 MLS 显示出与基于重量的硬度相比,硬度增加。这些 MLS 还将使用我们的低压 TEM 进行成像,以分析蛋白质-矿物质边界表面的超微结构差异,并将其与机械性能相关联。为了通过 TEM 深入了解病毒表面和内部通道的矿化情况,还计划组装小的病毒样颗粒 (VLP) 并将其矿化在垂直方向的阵列中。

项目成果

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Professor Dr. Joachim Bill其他文献

Professor Dr. Joachim Bill的其他文献

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{{ truncateString('Professor Dr. Joachim Bill', 18)}}的其他基金

Coordination Funds
协调基金
  • 批准号:
    210421515
  • 财政年份:
    2012
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Genetically optimized Tobacco mosaic viruses as scaffold for the in vitro generation of semiconductor bio/metal-oxide nanostructured architectures
基因优化的烟草花叶病毒作为体外生成半导体生物/金属氧化物纳米结构体系的支架
  • 批准号:
    210453926
  • 财政年份:
    2012
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Synthesis and characterization of paper-like, nanostructured electrodes for advanced secondary batteries
用于先进二次电池的纸状纳米结构电极的合成和表征
  • 批准号:
    208744363
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
    Research Grants
In vivo und in vitro Synthesen von Oxidkeramiken
氧化物陶瓷的体内和体外合成
  • 批准号:
    112803380
  • 财政年份:
    2009
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Growth mechanism of two-component titania/vanadium oxide thin films from aqueous solutions
双组分二氧化钛/氧化钒薄膜在水溶液中的生长机理
  • 批准号:
    28197821
  • 财政年份:
    2006
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Synthesis and property characterization of precursor-derived ceramics reinforced by functionalized single-wall carbon nanotubes
功能化单壁碳纳米管增强前驱体陶瓷的合成及性能表征
  • 批准号:
    16687262
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Nanomechanical characterisation of multilayered organic-inorganic composite films produced by bioinspired processing routes
通过仿生加工路线生产的多层有机-无机复合薄膜的纳米力学表征
  • 批准号:
    15068179
  • 财政年份:
    2005
  • 资助金额:
    --
  • 项目类别:
    Research Grants
Synthese keramischer Festkörper im quaternären System Si-B-C-N aus molekularen Vorstufen
由分子前驱体合成四元体系 Si-B-C-N 陶瓷固体
  • 批准号:
    5390400
  • 财政年份:
    1997
  • 资助金额:
    --
  • 项目类别:
    Priority Programmes
Generation of luminescent materials through living micoalgae
通过活微藻产生发光材料
  • 批准号:
    246899901
  • 财政年份:
  • 资助金额:
    --
  • 项目类别:
    Research Grants

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具有低驱动电压特性的蓝光发射晶态OLED研究
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用于低场便携式 MRI 的可拉伸线圈
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使用低成本、多功能测量 (Versametrics) 系统能够研究生物分子的电转换信息
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