An Implantable Biosensor Platform Enabled by Novel Porous Oxide Protection of Electrochemical Aptamer Working Electrodes

由电化学适体工作电极的新型多孔氧化物保护实现的植入式生物传感器平台

• 批准号: 2327102
• 负责人: Jason Heikenfeld
• 金额: $ 45万
• 依托单位: University of Cincinnati Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2026-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2327102&HistoricalAwards=false
• 关键词: Implantable; Biosensor; Platform; Enabled; Novel

Managing chronic diseases in the United States is the biggest cost to the health care system and is poised to worsen as the population ages. Not patients, not doctors, and certainly not insurance companies are satisfied with the complexity of care and patient outcomes for chronic diseases. However, improved care in chronic disease has without question been achieved with the wide-spread use of wearable monitoring of glucose for diabetics. The impact of continuous wearable monitoring has been so substantial for heart disease and for diabetes that there is already a commercial emergence of long-lasting implantable monitors for glucose and heart function. However, only a small number of measures are possible with implantable sensors due to the ultra-difficult requirement of the sensors having to last a year or more of continuous operation. This is unfortunate, because implanted monitors can in theory reduce costs (less trips to the doctor’s office for tests), improve patient outcomes by more timely and actionable data, and by virtue of their simplicity (almost no user effort) have greater impact on patient lifestyle choices. A need therefore exists for a new technology that can not only measure many types of molecules in the body, but do so with reliable operation for a year or more such that implantable use becomes practical and desirable.The specific objective of this project is to demonstrate the fundamental building blocks needed for molecular sensors to monitor inside the body for multiple years, and therefore enable the first ever implantable monitoring platform that is generalizable to multiple chronic diseases. The project work plan focuses on aptamer sensors, which place strands of DNA on an electrode, and the DNA captures and allows electronic measurement of molecules circulating throughout the body. These aptamer sensors essentially provide the same type of information achieved with a blood test at the doctor’s office, but instead will be measuring all the time inside the body. Making these sensors last long enough for implanted operation is a major challenge and is the specific scientific focus of this project. To enable more than one year of operation this project will stabilize the sensor surface with robust porous oxides with a goal of 1 month of reliable operation, then develop a device design with 12 sensors that are sequentially exposed at one-month intervals inside the body such that at least 12 months of operation is achieved. Achieving 12-month operation then opens the possibility for an implanted sensor, where patients and doctors have continuous access to health status and a simpler and more impactful way to manage a patient’s chronic disease.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在美国，管理慢性病是医疗保健系统的最大成本，并且随着人口老龄化，这种成本将会进一步恶化。患者、医生、当然保险公司都不满意慢性病护理的复杂性和患者的治疗结果。然而，随着糖尿病患者可穿戴式血糖监测的广泛使用，慢性疾病的护理无疑得到了改善。连续可穿戴式监测对心脏病和糖尿病的影响如此之大，以至于已经出现了商业化。持久的植入式血糖监测仪然而，由于传感器必须持续运行一年或更长时间，因此植入式传感器只能进行少量测量，这是不幸的，因为植入式监测仪理论上可以降低成本。减少前往医生办公室进行测试的次数），通过更及时和可操作的数据来改善患者的治疗结果，并且凭借其简单性（几乎不需要用户的努力）对患者的生活方式选择产生更大的影响，因此需要一种新技术。不仅可以测量多种类型的分子在体内，但要可靠运行一年或更长时间，以便植入式使用变得实用和理想。该项目的具体目标是展示分子传感器在体内监测多年所需的基本构建模块，因此，实现了第一个可适用于多种慢性疾病的植入式监测平台。该项目工作计划重点关注适体传感器，将 DNA 链放置在电极上，DNA 捕获并允许对全身循环的分子进行电子测量。这些适体传感器本质上提供与在医生办公室进行血液测试获得的信息相同，但要在体内一直进行测量，使这些传感器能够持续足够长的时间以进行植入手术是一项重大挑战，也是该项目的具体科学重点。为了实现一年以上的运行，该项目将使用坚固的多孔氧化物稳定传感器表面，目标是可靠运行 1 个月，然后开发一种包含 12 个传感器的设备设计，这些传感器每隔一个月依次暴露在体内，例如至少 12 个月实现 12 个月的手术后，植入式传感器成为可能，患者和医生可以持续了解健康状况，并以更简单、更有效的方式来管理患者的慢性病。该奖项反映了 NSF 的法定使命，并具有通过使用基金会的智力优点和更广泛的影响审查标准进行评估，被认为值得支持。