DREAM Sentinels: Multiplexable and programmable cell-free ADAR-mediated RNA sensing platform (cfRADAR) for quick and scalable response to emergent viral threats
DREAM Sentinels:可复用且可编程的无细胞 ADAR 介导的 RNA 传感平台 (cfRADAR),可快速、可扩展地响应突发病毒威胁
基本信息
- 批准号:2319913
- 负责人:
- 金额:$ 70万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2024
- 资助国家:美国
- 起止时间:2024-01-01 至 2026-12-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
This project seeks to address a critical public health need: the rapid, precise detection of viral RNA, the genetic blueprint of many viruses, including the SARS-CoV-2 virus responsible for COVID-19. The approach is to develop a cutting-edge tool known as "cell-free reprogrammable adenosine deaminases acting on RNA" (cfRADAR). This tool functions as a highly adaptable virus detective, designed to be quickly modified to recognize new or mutating viruses. Beyond its primary function, cfRADAR is projected to have broader societal impacts. Firstly, it can significantly improve healthcare outcomes by providing real-time information on viral prevalence and spread, enabling earlier detection, prompt responses to outbreaks, and ultimately reducing the risk of transmission. This reliable, rapid diagnostic tool can enhance our capabilities to prevent, control, and treat infectious diseases. Secondly, the project will engage in outreach and educational activities, offering hands-on experiences and interactive learning for students and teachers at various levels. Therefore, cfRADAR's development stands to significantly benefit both local and global communities, serving the national interest by advancing health, prosperity, and welfare.This project's primary objective is the development of cfRADAR (cell-free reprogrammable adenosine deaminases acting on RNA), an RNA detection technology leveraging the properties of adenosine deaminases acting on RNA (ADAR). The project will tackle six main goals: 1) developing singleplex and 2) multiplex cfRADAR assays, 3) creating cfRADAR-based logic circuits for group testing, 4) formulating field deployable lyophilized cfRADAR systems, 5) designing a field deployable portable analyzer, and 6) validating cfRADAR in an infrastructure-free setting. The cfRADAR platform offers advantages such as high sensitivity, reprogrammability, modularity, and field deployability. With its single-target transcript to multiple sensor molecule editing, cfRADAR possesses high sensitivity towards low-abundance RNA targets. Its modularity and reprogrammability allow for the use of Boolean logical gates, offering the detection of multiple RNA targets simultaneously. The exploration of lyophilized cell-free reagents and portable analyzers enhances the platform's field deployment, making it an effective tool for timely response against viral threats. The project could revolutionize point-of-care diagnosis, group tests, and biosurveillance by introducing a sensitive, reprogrammable, and scalable sensing platform.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
该项目旨在满足一项关键的公共卫生需求:快速、精确地检测病毒 RNA,这是许多病毒的遗传蓝图,包括导致 COVID-19 的 SARS-CoV-2 病毒。该方法是开发一种名为“作用于 RNA 的无细胞可重编程腺苷脱氨酶”(cfRADAR) 的尖端工具。该工具充当高度适应性的病毒侦探,旨在快速修改以识别新的或变异的病毒。除了其主要功能之外,cfRADAR 预计还将产生更广泛的社会影响。首先,它可以通过提供有关病毒流行和传播的实时信息、实现早期检测、迅速应对疫情并最终降低传播风险,显着改善医疗保健结果。这种可靠、快速的诊断工具可以增强我们预防、控制和治疗传染病的能力。其次,该项目将开展外展和教育活动,为各级学生和教师提供实践经验和互动学习。因此,cfRADAR 的开发将使当地和全球社区受益匪浅,通过促进健康、繁荣和福利来服务于国家利益。该项目的主要目标是开发 cfRADAR(作用于 RNA 的无细胞可重编程腺苷脱氨酶),这是一种 RNA利用作用于 RNA 的腺苷脱氨酶 (ADAR) 特性的检测技术。该项目将实现六个主要目标:1) 开发单重和 2) 多重 cfRADAR 测定,3) 创建基于 cfRADAR 的逻辑电路用于组测试,4) 制定可现场部署的冻干 cfRADAR 系统,5) 设计可现场部署的便携式分析仪,以及6) 在无基础设施的环境中验证 cfRADAR。 cfRADAR 平台具有高灵敏度、可重编程性、模块化和现场可部署性等优势。凭借其单靶标转录到多传感器分子编辑的功能,cfRADAR 对低丰度 RNA 靶标具有高灵敏度。其模块化性和可重编程性允许使用布尔逻辑门,同时检测多个 RNA 靶标。对冻干无细胞试剂和便携式分析仪的探索增强了该平台的现场部署,使其成为及时应对病毒威胁的有效工具。该项目可以通过引入敏感、可重新编程和可扩展的传感平台,彻底改变即时诊断、群体测试和生物监测。该奖项反映了 NSF 的法定使命,并通过利用基金会的智力优势和更广泛的评估进行评估,被认为值得支持。影响审查标准。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
High Fidelity Machine-Learning-Assisted False Positive Discrimination in Loop-Mediated Isothermal Amplification Using Nanopore-Based Sizing and Counting
使用基于纳米孔的尺寸确定和计数,在循环介导的等温扩增中进行高保真机器学习辅助的假阳性辨别
- DOI:10.1021/acsnano.3c12053
- 发表时间:2024-03
- 期刊:
- 影响因子:17.1
- 作者:Dong, Ming;Kshirsagar, Aneesh;Politza, Anthony J.;Guan, Weihua
- 通讯作者:Guan, Weihua
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Weihua Guan其他文献
Developing A Baseline Metabolomic Signature Associated with COVID-19 Severity: Insights from Prospective Trials Encompassing 13 U.S. Centers
开发与 COVID-19 严重程度相关的基线代谢组特征:来自美国 13 个中心的前瞻性试验的见解
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:4.1
- 作者:
Kaifeng Yang;Zhiyu Kang;Weihua Guan;Sahar Lotfi;Zachary J. Mayer;Candace R. Guerrero;B. Steffen;M. Puskarich;Christopher J. Tignanelli;Elizabeth R Lusczek;S. Safo - 通讯作者:
S. Safo
Research on the health status and influencing factors of the older adult floating population in Shanghai
上海市老年人流动人口健康状况及影响因素研究
- DOI:
10.3389/fpubh.2024.1361015 - 发表时间:
2024-05-22 - 期刊:
- 影响因子:5.2
- 作者:
Lianxia Wu;Wei Li;Shaogu Wang;Weihua Guan;Xianyu Wang - 通讯作者:
Xianyu Wang
Examination of newborn DNA methylation among women with polycystic ovary syndrome/hirsutism
多囊卵巢综合征/多毛症女性新生儿DNA甲基化检查
- DOI:
- 发表时间:
2023 - 期刊:
- 影响因子:3.7
- 作者:
K. Polinski;S. Robinson;D. Putnick;R. Sundaram;E. Bell;P. Joseph;James Segars;Weihua Guan;Robert M. Silver;E. Schisterman;Sunni L. Mumford;E. Yeung - 通讯作者:
E. Yeung
Toxoplasma gondii infection triggers ongoing inflammation mediated by increased intracellular Cl- concentration in airway epithelium.
弓形虫感染会引发气道上皮细胞内 Cl- 浓度增加介导的持续炎症。
- DOI:
10.1016/j.jinf.2022.10.037 - 发表时间:
2022-11-01 - 期刊:
- 影响因子:0
- 作者:
Zhuo;Lei Chen;X. Hou;Jie Sheng;Jian;Jia;Dong;Ze;Tian;Ziwen Huang;Lei Peng;Hai Yang;Qin;Yun;Weihua Guan;Z. Lun;Wen;Yi - 通讯作者:
Yi
Weihua Guan的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Weihua Guan', 18)}}的其他基金
CAREER: Amplification-Coupled Solid-State Nanopore Digital Counting based a Versatile Platform for Point-of-Care Nucleic Acid Testing
职业:基于扩增耦合固态纳米孔数字计数的多功能平台,用于即时核酸检测
- 批准号:
2045169 - 财政年份:2021
- 资助金额:
$ 70万 - 项目类别:
Continuing Grant
Quantitative microfluidic NAT-on-USB: towards routine HIV viral load testing
定量微流控 NAT-on-USB:走向常规 HIV 病毒载量检测
- 批准号:
1912410 - 财政年份:2019
- 资助金额:
$ 70万 - 项目类别:
Standard Grant
Ultracompact sample-to-answer nucleic acid test on USB stick
USB 记忆棒上的超紧凑样本到答案核酸测试
- 批准号:
1902503 - 财政年份:2019
- 资助金额:
$ 70万 - 项目类别:
Standard Grant
Nanofluidic Charge Coupled Devices for Molecular Separation and Sensing
用于分子分离和传感的纳流体电荷耦合器件
- 批准号:
1710831 - 财政年份:2017
- 资助金额:
$ 70万 - 项目类别:
Standard Grant
相似海外基金
EAGER SENTINELS: The PCR-free Biosensor for a Fast, Simple, and Sensitive Detection of RNA.
EAGER SENTINELS:无需 PCR 的生物传感器,可快速、简单且灵敏地检测 RNA。
- 批准号:
2415037 - 财政年份:2024
- 资助金额:
$ 70万 - 项目类别:
Standard Grant
Collaborative Research: From Peaks To Slopes To Communities, Tropical Glacierized Volcanoes As Sentinels of Global Change: Integrated Impacts On Water, Plants and Elemental Cycling
合作研究:从山峰到斜坡到社区,热带冰川火山作为全球变化的哨兵:对水、植物和元素循环的综合影响
- 批准号:
2317854 - 财政年份:2023
- 资助金额:
$ 70万 - 项目类别:
Continuing Grant
Collaborative Research: From Peaks To Slopes To Communities, Tropical Glacierized Volcanoes As Sentinels of Global Change: Integrated Impacts On Water, Plants and Elemental Cycling
合作研究:从山峰到斜坡到社区,热带冰川火山作为全球变化的哨兵:对水、植物和元素循环的综合影响
- 批准号:
2317852 - 财政年份:2023
- 资助金额:
$ 70万 - 项目类别:
Continuing Grant
DREAM Sentinels: Selection of aptamers that target viral variants with high specificity
DREAM Sentinels:选择高特异性针对病毒变体的适体
- 批准号:
2235455 - 财政年份:2023
- 资助金额:
$ 70万 - 项目类别:
Continuing Grant
Collaborative Research: From Peaks To Slopes To Communities, Tropical Glacierized Volcanoes As Sentinels of Global Change: Integrated Impacts On Water, Plants and Elemental Cycling
合作研究:从山峰到斜坡到社区,热带冰川火山作为全球变化的哨兵:对水、植物和元素循环的综合影响
- 批准号:
2317851 - 财政年份:2023
- 资助金额:
$ 70万 - 项目类别:
Continuing Grant