NSF-BSF: IIBR Instrumentation: Photonic Band Gap Resonators for High-Field Dynamic Nuclear Polarization of Biological Macromolecules

NSF-BSF：IIBR 仪器：用于生物大分子高场动态核极化的光子带隙谐振器

• 批准号: 2311042
• 负责人: Alexander Nevzorov
• 金额: $ 99.84万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2027-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2311042&HistoricalAwards=false
• 关键词: NSF; BSF; IIBR; Instrumentation; Photonic

An award is made to North Carolina State University (NCSU, USA) with support from the Infrastructure Innovation Program for Biological Research in the Division of Biological Infrastructure and the Chemical Measurement and Imaging Program in the Division of Chemistry to considerably – by up to several orders of magnitude – improve sensitivity of Nuclear Magnetic Resonance (NMR) spectroscopy by employing pulsed high-frequency methods of Dynamic Nuclear Polarization (DNP). This first-of-its kind spectrometer will be developed in partnership with Tel Aviv University (TAU, Israel), thereby strengthening scientific collaboration between the two Nations. Such gains in sensitivity will expand the applicability of NMR methods to some of the most challenging problems of structural biology and, potentially, make the method suitable for studying protein structure and function in living cells. This highly interdisciplinary collaborative project will provide unique training opportunities for students with backgrounds in biology, physical chemistry, spin physics, and millimeter-wave technologies.The project is aimed at transforming DNP NMR methods by significantly expanding the photonic band-gap resonator technology invented at NCSU from the current 200 GHz electron resonance frequency to 400 GHz and take advantage of the expertise of the TAU team in pulse shaping and cryoprobe development. The instrument will operate at 400 GHz electron and 600 MHz proton NMR frequencies, which will be highly beneficial for higher resolution and sensitivity. Coherent manipulation of the electronic spin states will be achieved by combining state-of-the- art digital technologies and recent advances in solid-state millimeter-wave devices. The spectrometer will serve as a unique platform for developing new methods for transferring spin polarization in DNP. The method is expected to yield novel structural and dynamic information on biological macromolecules as compared to conventional NMR spectroscopy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在生物基础设施部门的生物研究基础设施创新计划和化学部门的化学测量和成像计划的支持下，北卡罗来纳州立大学（NCSU，美国）获得了高达数个奖项幅度 - 通过采用动态核极化 (DNP) 脉冲高频方法提高核磁共振 (NMR) 光谱的灵敏度 这种首创的光谱仪将与 Tel 合作开发。阿维夫大学（TAU，以色列），从而加强两国之间的科学合作，这种灵敏度的提高将扩大核磁共振方法在结构生物学中一些最具挑战性问题的适用性，并有可能使该方法适合研究蛋白质结构。这个高度跨学科的合作项目将为具有生物学、物理化学、自旋物理和毫米波技术背景的学生提供独特的培训机会。该项目旨在通过显着扩展光子波段来改变 DNP NMR 方法。 -间隙谐振器NCSU 发明的技术从当前的 200 GHz 电子共振频率到 400 GHz，并利用 TAU 团队在脉冲整形和冷冻探针开发方面的专业知识，该仪器将在 400 GHz 电子和 600 MHz 质子 NMR 频率下运行。通过结合最先进的数字技术和固态毫米波器件的最新进展，可以实现电子自旋态的相干操纵。光谱仪将作为开发 DNP 中自旋极化转移新方法的独特平台，与传统 NMR 光谱相比，该方法有望产生生物大分子的新颖结构和动态信息。该奖项反映了 NSF 的法定使命，并被认为是值得的。通过使用基金会的智力优势和更广泛的影响审查标准进行评估来获得支持。