Exceptional Points Enhanced Acoustic Sensing of Biological Cells

特殊点增强生物细胞的声学传感

• 批准号: 2328407
• 负责人: Xiaoyun Ding
• 金额: $ 56.85万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2328407&HistoricalAwards=false
• 关键词: Exceptional; Points; Enhanced; Acoustic; Sensing

This award supports research that investigates the fundamentals understanding of a novel biosensing concept for high-sensitivity measurement of cell mechanical biomarkers. Biomarkers, or biological markers, are measurable indicators of a biological cell state or condition and can be closely associated with changes in functionality and health of cells. Thus, the ability to measure multiple cell mechanical properties as biomarkers will benefit biological research and clinical applications such as cancer studies, drug screening/discovery, and immunology. The acoustic sensing concept in this project relies on the detection of exceptional points, which are highly sensitive dynamic states that occur in non-Hermitian physical systems characterized by balanced gain and loss. The long-term research goal of the project is to employ the sensing scheme for measuring mechanical biomarkers of individual cells in high throughput for both fundamental studies and clinical applications. The research findings will also contribute to the PIs’ long-term education and outreach goals of motivating students toward science and engineering, by illustrating far reaching implications of engineering. The project activities will also specifically illustrate how engineers contribute to biomedicine through acoustic sensing and fundamental wave mechanics concepts. Results from this research will benefit the US health and society, impacting the fields of acoustic sensing, disease diagnosis, and cellular biology.The project will investigate fundamental research questions related to how biological cells with different mechanical properties (stiffness, density, compressibility and viscosity) interact with acoustic waves propagating along a substrate, how perturbations caused by the cell and by changes in its properties can break the dynamic equilibrium associated with exceptional points, and how the corresponding frequency splitting mechanism can quantify changes in the cell properties and thus form the basis for a novel acoustic biosensing technique. The project objectives will be accomplished through two research tasks: (1) investigation of cell-wave interaction and fundamental studies on exceptional point generation in acoustic substrates; and (2) experimental investigations, and proof-of-concept of exceptional point-based acoustic biosensing. This project will lead to advances in the understanding of the interaction between surface acoustic waves and a cell. The study of exceptional points generation in surface acoustic waves substrates with a cavity is a novel aspect of the work, which will explore the interaction of multiple wave modes with cavity-cell resonances, their coupling and hybridization. Analytical, numerical, and experimental studies will explore the sensitivity of exceptional points sensing of the cell’s physical properties, which will open new pathways for sensing.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项支持研究研究细胞机械生物标志物的高敏性测量的新型生物传感概念的基础知识的研究。生物标志物或生物标记物是生物细胞状态或状况的可测量指标，并且可能与细胞功能和健康的变化密切相关。这就是测量多个细胞机械性能作为生物标志物的能力将使生物学研究和临床应用有益于癌症研究，药物筛查/发现和免疫学。该项目中的声传感概念依赖于特殊点的检测，这些点是高度敏感的动态状态，这些状态在以平衡的增益和损失为特征的非热式物理系统中发生。该项目的长期研究目标是采用传感方案来测量基本研究和临床应用中高吞吐量中单个细胞的机械生物标志物。研究结果还将通过说明工程学的遥远影响，为PIS的长期教育和激励学生迈向科学和工程学的目标做出贡献。项目活动还将特别说明工程师如何通过声学传感和基本波力力学概念为生物医学贡献。这项研究的结果将使美国的健康和社会受益，影响声学感应，疾病诊断和细胞生物学领域。该项目将调查与与沿A型和通过渗透率相关的动力相关的且与驱散性的范围相关的范围，并与沿A异型相关的动力相互作用，与其在A ISTRATION中相互作用的生物学细胞如何相关的生物学细胞如何相关的生物学细胞与该属性相互作用，并在其范围内相互作用。相应的频率分裂机制如何量化细胞性质的变化，从而构成新型声学生物传感技术的基础。项目目标将通过两项研究任务来实现：（1）细胞波相互作用的投资和对声学基质中特殊点产生的基本研究； （2）实验投资，以及基于点的声学生物传感的概念验证。该项目将导致对表面声波与细胞之间相互作用的理解的进步。研究具有空腔的表面声波底物中的特殊点产生是工作的一个新方面，它将探索多个波模式与腔细胞共振的相互作用，它们的耦合和杂交。分析，数值和实验研究将探讨细胞物理特性的特殊点灵敏度的灵敏度，这将为敏感性打开新的途径。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的审查标准来通过评估来获得支持的。