LEAPS-MPS: Water-soluble Afterglow Luminescent Nanocomposites for Bioimaging and Bioanalysis

LEAPS-MPS：用于生物成像和生物分析的水溶性余辉发光纳米复合材料

• 批准号: 2316812
• 负责人: Steven Wu
• 金额: $ 24.91万
• 依托单位: University of South Dakota Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2316812&HistoricalAwards=false
• 关键词: LEAPS; MPS; Water; soluble; Afterglow

In this project, co-funded by the Directorate for Mathematical and Physical Sciences (MPS) and the Established Program to Stimulate Competitive Research (EPSCoR), and managed by the Chemistry Division at NSF, Steven Wu and his students at the University of South Dakota will perform studies that aim to develop a range of water-soluble afterglow luminescent nanocomposites for bioimaging and bioanalysis, using carbon dots (CDs) and semiconducting polymer nanoparticles (SPNs). Due to the strong autofluorescence from biosamples and limited light penetration depth, it is still a challenge to eliminate background noise during optical bioimaging and bioanalysis to achieve high sensitivity and accuracy. Dr. Wu and his students will investigate the synthesis, characterization, and afterglow luminescent mechanisms of the CDs@SPN nanocomposites and will work to demonstrate their potential in bioimaging and bioanalysis. Their studies are expected to provide insight into the design of afterglow luminescent probes with reduced background noise for bioimaging and bioanalysis, thereby improving detection sensitivity and accuracy. In addition, Dr. Wu and his team will reach out to Tribal Colleges and Universities in South Dakota and provide opportunities for underrepresented undergraduate students to participate in research projects and encourage them to consider science as a career option. Under this award, the Wu research team at the University of South Dakota proposes to address the challenges of strong background noise during optical bioimaging and bioanalysis using afterglow luminescent nanomaterials. They will perform chemical synthesis, characterization and mechanistic investigations of the afterglow nanomaterials that they are developing for bioimaging applications. The technical objectives of this project are four-fold: (i) synthesis of of a spectrum of afterglow carbon dots by adjusting the doping elements and carbonization level; (ii) synthesis of afterglow carbon dot-doped semiconducting polymer nanoparticles (CD@SPNs), which could constitute a general approach to fabricating water-soluble afterglow luminescent probes based on Fӧrster resonance energy transfer (FRET) between CDs and SPNs; (iii) investigating the mechanism of afterglow luminescence and (iv) implementation of afterglow luminescent nanoprobes in bioimaging and bioanalysis to achieve high sensitivity and accuracy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在这个项目中，由数学和物理科学理事会 (MPS) 和刺激竞争性研究既定计划 (EPSCoR) 共同资助，并由 NSF 化学部门管理，Steven Wu 和他在南达科他大学的学生将进行研究，旨在使用碳点（CD）和半导体聚合物纳米颗粒开发一系列用于生物成像和生物分析的水溶性余辉发光纳米复合材料由于生物样品的强自发荧光和有限的光穿透深度，在光学生物成像和生物分析过程中消除背景噪声以实现高灵敏度和准确性仍然是一个挑战，吴博士和他的学生将研究合成、表征。以及 CDs@SPN 纳米复合材料的余辉发光机制，并将致力于展示其在生物成像和生物分析中的潜力，他们的研究有望为背景噪声降低的余辉发光探针的设计提供深入的见解。此外，吴博士和他的团队将与南达科他州的部落学院和大学合作，为代表性不足的本科生提供参与研究项目的机会，并鼓励他们将科学视为一门学科。根据该奖项，南达科他大学的吴研究团队提出利用余辉发光纳米材料来解决光学生物成像和生物分析过程中强背景噪声的挑战，他们将进行化学合成、表征和机理研究。他们正在开发用于生物成像应用的余辉纳米材料的研究，该项目的技术目标有四个：（i）通过调整掺杂元素和碳化水平合成一系列余辉碳点；（ii）余辉碳点的合成。碳点掺杂的半导体聚合物纳米粒子（CD@SPNs），它可以构成基于费斯特共振能量转移制造水溶性余辉发光探针的通用方法CD 和 SPN 之间的荧光共振能量转移 (FRET)；(iii) 研究余辉发光机制；(iv) 在生物成像和生物分析中实施余辉发光纳米探针，以实现高灵敏度和准确性。该奖项反映了 NSF 的法定使命，并被认为值得支持通过使用基金会的智力优点和更广泛的影响审查标准进行评估。