Regulation of pre-mRNA splicing by the circadian system

昼夜节律系统对前体 mRNA 剪接的调节

• 批准号: 2309854
• 负责人: Stacey Harmer
• 金额: $ 110万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2309854&HistoricalAwards=false
• 关键词: Regulation; pre; mRNA; splicing; circadian

The survival of plants depends upon their ability to anticipate and respond appropriately to environmental challenges such as changes in light, temperature, and encounters with pests and pathogens. A key regulator of the timing and magnitude of responses to such challenges is the plant circadian clock. Advantageous variants of components of this internal timekeeper have been selected by farmers during the domestication of many crops; further selection of clock components will be important as we adapt existing crop species to a changing climate. A better understanding of the nature of the plant circadian clock and how it interacts with fundamental cellular processes is required to expedite such efforts. The project supported by this award will investigate the relationship between the plant circadian clock and RNA processing, providing valuable insights into both these highly conserved and essential processes. In addition, the project will help train a new generation of scientists, engaging high school and undergraduate students as well as graduate students and post-doctoral scholars. An important component will be a research-based course for first-year college students; such courses have been shown to increase persistence of students in science and enhance their understanding of concepts and development of skills.The overall objective of this project is to understand how the circadian system and RNA generation and processing pathways interact with each other. The abundance of approximately one-third of transcripts in Arabidopsis thaliana are regulated by the circadian oscillator. However, the respective contributions of circadian control of transcription, RNA processing, and RNA export from the nucleus on these rhythms have not been investigated. Moreover, the relative influence of these processes on normal circadian function is still being clarified. This project will use genomic, genetic, biochemical, and modeling methods to investigate the connections between pre-mRNA processing and the circadian system, the role of splicing kinetics in the circadian clock, and the functional relationship between XCT and PRP19, two factors highly conserved across eukaryotes that play important roles in both RNA processing and circadian clock function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物的生存取决于它们预测和适当应对环境挑战的能力，例如光照、温度的变化以及害虫和病原体的遭遇。植物生物钟是调节应对此类挑战的时间和幅度的关键因素。农民在许多作物的驯化过程中选择了这种内部计时器组件的有利变体；当我们使现有作物品种适应不断变化的气候时，进一步选择时钟组件将非常重要。为了加快这些努力，需要更好地了解植物生物钟的性质以及它如何与基本细胞过程相互作用。该奖项支持的项目将研究植物生物钟和 RNA 加工之间的关系，为这些高度保守且重要的过程提供有价值的见解。此外，该项目还将帮助培养新一代科学家，吸引高中生、本科生以及研究生和博士后学者。一个重要的组成部分将是为一年级大学生开设的研究型课程；此类课程已被证明可以提高学生对科学的坚持，增强他们对概念的理解和技能的发展。该项目的总体目标是了解昼夜节律系统和 RNA 生成和处理途径如何相互作用。拟南芥中大约三分之一转录本的丰度受到昼夜节律振荡器的调节。然而，转录、RNA 加工和核 RNA 输出的昼夜节律控制对这些节律的各自贡献尚未得到研究。此外，这些过程对正常昼夜节律功能的相对影响仍有待阐明。该项目将利用基因组、遗传、生物化学和建模方法来研究前体mRNA加工与昼夜节律系统之间的联系、剪接动力学在昼夜节律钟中的作用，以及XCT和PRP19这两个高度保守的因子之间的功能关系。在 RNA 加工和生物钟功能中发挥重要作用的真核生物。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响进行评估，被认为值得支持审查标准。