Regulation of pre-mRNA splicing by the circadian system

昼夜节律系统对前体 mRNA 剪接的调节

• 批准号: 2309854
• 负责人: Stacey Harmer
• 金额: $ 110万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2309854&HistoricalAwards=false
• 关键词: Regulation; pre; mRNA; splicing; circadian

The survival of plants depends upon their ability to anticipate and respond appropriately to environmental challenges such as changes in light, temperature, and encounters with pests and pathogens. A key regulator of the timing and magnitude of responses to such challenges is the plant circadian clock. Advantageous variants of components of this internal timekeeper have been selected by farmers during the domestication of many crops; further selection of clock components will be important as we adapt existing crop species to a changing climate. A better understanding of the nature of the plant circadian clock and how it interacts with fundamental cellular processes is required to expedite such efforts. The project supported by this award will investigate the relationship between the plant circadian clock and RNA processing, providing valuable insights into both these highly conserved and essential processes. In addition, the project will help train a new generation of scientists, engaging high school and undergraduate students as well as graduate students and post-doctoral scholars. An important component will be a research-based course for first-year college students; such courses have been shown to increase persistence of students in science and enhance their understanding of concepts and development of skills.The overall objective of this project is to understand how the circadian system and RNA generation and processing pathways interact with each other. The abundance of approximately one-third of transcripts in Arabidopsis thaliana are regulated by the circadian oscillator. However, the respective contributions of circadian control of transcription, RNA processing, and RNA export from the nucleus on these rhythms have not been investigated. Moreover, the relative influence of these processes on normal circadian function is still being clarified. This project will use genomic, genetic, biochemical, and modeling methods to investigate the connections between pre-mRNA processing and the circadian system, the role of splicing kinetics in the circadian clock, and the functional relationship between XCT and PRP19, two factors highly conserved across eukaryotes that play important roles in both RNA processing and circadian clock function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物的生存取决于它们预测和应对环境挑战的能力，例如光，温度的变化以及与害虫和病原体相遇的能力。对这种挑战的时间和响应量的关键调节器是植物昼夜节律的时钟。在许多农作物的驯化过程中，农民选择了这种内部计时器的有利变体。随着我们将现有的农作物物种调整为不断变化的气候，时钟组件的进一步选择将很重要。需要更好地理解植物昼夜节律时钟的性质及其与基本蜂窝过程的相互作用，以加快这种努力。该奖项支持的项目将调查植物昼夜节律与RNA处理之间的关系，从而为这些高度保守和基本过程提供宝贵的见解。此外，该项目将帮助培训新一代的科学家，吸引高中和本科生以及研究生和博士后学者。一个重要的组成部分是针对一年级大学生的研究课程；这些课程已被证明可以增加学生在科学领域的持久性，并增强他们对概念和技能发展的理解。该项目的总体目标是了解昼夜节律系统和RNA的产生和处理途径如何相互相互作用。拟南芥中大约三分之一的转录本的丰度受昼夜节律振荡器的调节。然而，尚未研究昼夜节律对转录，RNA加工和从核向这些节奏的RNA导出的各自的贡献。此外，这些过程对正常昼夜节律功能的相对影响仍在阐明。该项目将使用基因组，遗传，生化和建模方法来研究MRNA处理前与昼夜节律系统之间的联系，在昼夜节律中剪接动力学的作用以及XCT和PRP19之间的功能关系，这两个因素是在RNA统计中扮演着重要角色的两个因素，这两个因素都在RNA统计中扮演着统计的角色，并在RNA统计中扮演着统计的统计范围。认为值得通过基金会的智力优点和更广泛影响的评论标准来评估值得支持。