Elucidating Bio-Nano Interface Atomic Structure and Peptide Directed Nanoparticle Formation

阐明生物纳米界面原子结构和肽引导纳米颗粒的形成

• 批准号: 2304833
• 负责人: Gregory Holland
• 金额: $ 47.27万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-15 至 2026-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2304833&HistoricalAwards=false
• 关键词: Elucidating; Bio; Nano; Interface; Atomic

With the support of the Macromolecular, Supramolecular and Nanochemistry Program in the Division of Chemistry, Gregory Holland of San Diego State University is developing atomic and molecular level structural understanding of nano-bio interfaces and the influence of nanoparticle curvature, mesoporosity and morphology on biomolecular ligand structure, dynamics and organization on nanoparticle surfaces. The inorganic cores and the biomolecular ligand coatings are structurally distinct entities but strongly influence each other synergistically determining the collective properties of the nanoparticle system. The systems of interest include a range of silica nanostructures with varying morphology and naturally inspired hydroxyapatite nanomaterials functionalized with protein- and lipid-based ligands. Elucidating the atomic, molecular and nanoscale detail of biomolecular assembly on nanomaterials, developing novel peptide-directed nanoparticle syntheses and understanding the surface chemistry of biomolecules on nanoparticles is the research focus. This fundamental chemical knowledge could contribute to the development of next generation devices and sensors that couple nanomaterials with biomolecular functionality. The project is creating valuable research training opportunities for students at various levels that is further integrated with outreach activity development. Dr. Holland is building a highly interdisciplinary research and education program on nanoparticle surface characterization involving broad participation of graduate, undergraduate and High School interns contributing to the training of a diverse workforce.The vast majority of chemically synthesized and naturally occurring nanostructures, regardless of their chemical compositions, inorganic core, morphology and crystallinity, are coated with a ligand shell. The primary goal of this research is to determine the atomic and molecular level structure of nano-bio interfaces and to exploit biomolecules, including peptides and lipids as ligands in nanoparticle synthetic strategies. There are two main foci: (1) to investigate biomolecules assembled on a range of nanostructured silicas including colloidal monodisperse spheres, highly defective, branched fumed silica, and mesoporous silica nanoparticles where the behavior of biomolecules encapsulated within nanopores is the focus; and (2) naturally inspired hydroxyapatite nanorods and nanosheets synthesized with peptides as the templating agents. A combination of physical/analytical techniques will be used in the research, including solid-state NMR spectroscopy, electron microscopy and molecular dynamics simulation (MDS) to determine the structure and assembly of peptides and lipids at the interface of these systems and elucidate the influence of nanoparticle curvature and morphology on ligand architecture. This work is expanding on the synthetic and characterization methods available to the global nanoscience community for improved fundamental understanding of nano-biostructures.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系高分子、超分子和纳米化学项目的支持下，圣地亚哥州立大学的 Gregory Holland 正在开发对纳米生物界面的原子和分子水平结构理解以及纳米粒子曲率、中孔率和形态对生物分子配体的影响纳米颗粒表面的结构、动力学和组织。无机核和生物分子配体涂层在结构上是不同的实体，但彼此强烈影响，协同决定纳米粒子系统的集体特性。感兴趣的系统包括一系列具有不同形态的二氧化硅纳米结构和用基于蛋白质和脂质的配体功能化的天然羟基磷灰石纳米材料。阐明纳米材料上生物分子组装的原子、分子和纳米级细节，开发新型肽引导纳米颗粒合成以及了解纳米颗粒上生物分子的表面化学是研究重点。这种基础化学知识可以有助于开发将纳米材料与生物分子功能结合起来的下一代设备和传感器。该项目正在为各个级别的学生创造宝贵的研究培训机会，并进一步与外展活动的发展相结合。 Holland 博士正在建立一个关于纳米颗粒表面表征的高度跨学科的研究和教育计划，涉及研究生、本科生和高中实习生的广泛参与，有助于培训多元化的劳动力。绝大多数化学合成和天然存在的纳米结构，无论其性质如何化学成分、无机核、形态和结晶度均被配体壳所包覆。这项研究的主要目标是确定纳米生物界面的原子和分子水平结构，并利用生物分子（包括肽和脂质）作为纳米颗粒合成策略中的配体。主要有两个重点：（1）研究在一系列纳米结构二氧化硅上组装的生物分子，包括胶体单分散球、高度缺陷的支化二氧化硅和介孔二氧化硅纳米粒子，其中封装在纳米孔内的生物分子的行为是重点； (2)以肽为模板剂合成的天然羟基磷灰石纳米棒和纳米片。研究中将结合物理/分析技术，包括固态核磁共振波谱、电子显微镜和分子动力学模拟（MDS），以确定这些系统界面处肽和脂质的结构和组装，并阐明其影响纳米颗粒曲率和形态对配体结构的影响。这项工作正在扩展全球纳米科学界可用的合成和表征方法，以提高对纳米生物结构的基本理解。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。