CAREER: Elucidating the Mechanism of Ionic Liquid-Coated Nanoparticle Interactions with Blood Components

职业：阐明离子液体涂层纳米粒子与血液成分相互作用的机制

• 批准号: 2236629
• 负责人: Eden Tanner
• 金额: $ 85.1万
• 依托单位: University of Mississippi
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-15 至 2028-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2236629&HistoricalAwards=false
• 关键词: CAREER; Elucidating; Mechanism; Ionic; Liquid

Understanding how nanomaterials interact with the human body is a critical step to their safe and effective use in medicine. When nanoparticles are injected into the bloodstream, they encounter a rich environment, replete with diverse kinds of cells and proteins. Dr. Eden Tanner and her team at the University of Mississippi have developed an ionic liquid nanoparticle coating technology which enables selective in situ “hitchhiking” of nanoparticles onto different kinds of blood cells. This CAREER project will investigate this phenomenon to understand why and how this hitchhiking occurs, what impact it has on the cell membranes, and measure the strength of the forces between the nanoparticle and the cell membrane. This research project will provide critical fundamental knowledge on the interaction of charged materials with components of the bloodstream. Supported by Dr. Tanner and her lab, the student participants will be recruited from the Increasing Minority Access to Graduate Education Rising Science Star Program. These cohorts of Mississippi-based African American undergraduate scholars will be intensively mentored and supported from their first day of college until graduation and beyond, preparing them to enter graduate school and for careers as scientific leaders. Participants will work with the PI to return to their high schools to deliver outreach activities, thereby creating a robust, supportive pipeline of scientists and ultimately diversifying the scientific workforce.The research objective of this CAREER program is to interrogate the interactions between ionic liquid-coated nanoparticles and blood components. The Tanner lab at the University of Mississippi has demonstrated that successful surface modification of poly(lactic-co-glycolic) acid nanoparticles with a choline carboxylic acid-based ionic liquid results in preferential adhesion of the ionic liquid-nanoparticles to different blood components in whole blood. A key innovation will be determining the dynamics and mechanisms of adhesion of the ionic liquid-polymeric nanoparticle composites onto blood cell surfaces. The project will consist of the following three objectives: advance knowledge on the mechanism of attachment between the ionic liquid-nanoparticles and the cell membrane (Objective 1), assess the impact of the hitchhiking on the cell membrane (Objective 2), and measure the strength of the attractive forces and the effect of hitchhiking on adhesion to the blood vessel wall (Objective 3). The PI will use a host of analytical techniques to accomplish these objectives, including molecular dynamics simulations, optical tweezers, liquid chromatography-mass spectrometry, and small angle X-ray scattering. The educational objective of this CAREER program is the establishment of the Increasing Minority Access to Graduate Education Rising Science Star Program, an intensive mentoring program that immerses cohorts of Mississippi’s African American Science Technology Engineering and Mathematics students in research from their first day of college until their last (3 participants a year, 15 total). Partnering with the existing National Science Foundation-funded Increasing Minority Access to Graduate Education program, the project’s educational approach will provide the Rising Science Star Scholars with a high degree of social, financial and academic support, paving their path to graduate school and a scientific career. The PI will work in partnership with the participants to travel back to their high schools to conduct outreach activities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

了解纳米材料与人体的相互作用是他们在血液中的安全使用的关键一步。纳米涂料技术可以使纳米粒子“搭便车”到不同种类的血液细胞上。带电的材料与血液的组成部分的相互作用。科学领导者。基于胆碱羧酸的离子液体在Onic纳米颗粒的优先粘附到不同的血液中。纳米颗粒和细胞膜（物镜1）在细胞膜上搭上钩（物镜2），并测量有吸引力的福克斯的强度以及搭便车对血管壁的粘附的影响（目标3）。分析技术来实现目标响亮的分子动力学模拟，光学镊子，液态色谱 - 质量光谱法和小角度X射线散射的较小的教育目标。密西西比州的非裔美国人科学技术工程和数学学生从彩色的第一天到最后一天（3个参与者，总共15个）。在社会，财务和加速支持方面，铺平了他们的研究生院和科学职业。使用Theda Tion的智力优点和更广泛的影响审查标准。

项目成果

Good's buffer based highly biocompatible ionic liquid modified PLGA nanoparticles for the selective uptake in cancer cells

Goods 缓冲液基于高度生物相容性离子液体修饰的 PLGA 纳米颗粒，用于癌细胞的选择性摄取

• DOI: 10.1039/d3qm00787a
• 发表时间: 2023
• 期刊: Materials Chemistry Frontiers
• 影响因子: 7
• 作者: Singh, Gagandeep;Dasanayake, Gaya S.;Chism, Claylee M.;Vashisth, Priyavrat;Kaur, Amandeep;Misra, Sandeep Kumar;Sharp, Joshua S.;Tanner, Eden E.
• 通讯作者: Tanner, Eden E.