Collaborative Research: Room-temperature Superfluorescence in Multi-fluorophore Protein Cages and Its Origins

合作研究：多荧光团蛋白笼中的室温超荧光及其起源

• 批准号: 2232718
• 负责人: Jodi Hadden-Perilla
• 金额: $ 37.5万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2232718&HistoricalAwards=false
• 关键词: Collaborative; Research; Room; temperature; Superfluorescence

Ordinary light from an incandescent lamp is emitted by the independent excitation and relaxation of atoms in the filament. Twice the number of emitters makes the light twice as bright. Atoms are no ordinary emitters and can synchronize when certain conditions are met. When synchronization happens, the ensemble becomes super-radiant, and doubling the number of emitters quadruples light intensity. Synchronizing quantum emitters is easier said than done, but can be achieved under certain conditions. In this project, hundreds of molecular dyes will be chemically bound to the protein shell of a small plant virus. After a fast excitation pulse, the dyes spontaneously synchronize and collectively emit a burst of intense light. This project will tease out the mechanism by which the virus scaffold promotes coupling and synchronization of the dyes, at room temperature. Project outcomes could lead to future technologies for high contrast bioimaging. The research project will be complemented by STEM outreach activities, including opportunities for students to explore the innovation to invention pipeline.The spatial scale and the symmetry afforded by a virus scaffold have not been sufficiently explored in relation to emergent behavior, mainly due to the lack of comparable structural control. The preservation of quantum coherence at room temperature is especially intriguing. Emission occurs after pulsed excitation, as a delayed intense burst of ∼ 10 ps duration. Such non-classical dynamics contrast those of the much slower conventional exponential decay of uncorrelated chromophores. As a consequence, optical power density at deep sub-wavelength spatial scale, maximum turnover rate, and signal-to-background ratio dramatically increase. The project’s goal will be pursued through a theory-experiment collaboration. Approaches will couple experimental spectroscopic and molecular biology manipulations with predictive all-atom simulations that examine the structure and emergent dynamics of engineered virus-like particles. Proof-of-principle experiments are envisioned that exploit super-radiance and illustrate improved microscopic imaging contrast in single-particle analysis applications. The outcomes of this project could inspire future biophotonic technologies, providing control and understanding of the relationship between molecular structure and dynamics, and improved photonic properties.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

白炽灯的普通光是通过灯丝中原子的独立激发和弛豫而发出的，两倍的发射器数量使光的亮度增加了一倍。原子不是普通的发射器，并且在满足某些条件时可以同步。整个系统变得超辐射，发射器数量增加一倍，光强度增加四倍，说起来容易做起来难，但在某些条件下是可以实现的。在该项目中，数百种分子染料将化学结合到小型植物病毒的蛋白质外壳上，在快速激发脉冲后，染料会自发同步并集体发出强光。该项目将阐明其机制。病毒支架在室温下促进染料的耦合和同步，该项目的成果可能会带来未来的高对比度生物成像技术。该研究项目将得到 STEM 推广活动的补充，包括为学生提供探索创新到发明管道的机会。空间病毒支架提供的尺度和对称性与突现行为的关系尚未得到充分探索，主要是由于结构控制的可比性，在脉冲激发后发生的量子相干性的保存尤其令人感兴趣。这种非经典动力学与不相关发色团的慢得多的传统指数衰减形成鲜明对比，因此，深亚波长空间尺度的光功率密度、最大周转率和该项目的目标将通过理论实验合作来实现，方法是将实验光谱和分子生物学操作与预测性全原子模拟相结合，以检查工程病毒样颗粒的结构和涌现动力学。预计原理验证实验将利用超辐射并说明单粒子分析应用中显微成像对比度的改善，该项目的结果可能会启发未来的生物光子技术，提供对这种关系的控制和理解。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

AMBERff at Scale: Multimillion-Atom Simulations with AMBER Force Fields in NAMD.

• DOI: 10.1021/acs.jcim.3c01648
• 发表时间: 2024-01-22
• 期刊: JOURNAL OF CHEMICAL INFORMATION AND MODELING
• 影响因子: 5.6
• 作者: Antolinez, Santiago;Jones, Peter Eugene;Phillips, James C.;Hadden-Perilla, Jodi A.
• 通讯作者: Hadden-Perilla, Jodi A.