BRITE Pivot: Investigating the Role of Collagen Piezoelectricity in Biomineralization Enhanced by Force Inputs

BRITE Pivot：研究胶原蛋白压电性在力输入增强的生物矿化中的作用

• 批准号: 2227527
• 负责人: Hanna Cho
• 金额: $ 54.8万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2227527&HistoricalAwards=false
• 关键词: BRITE; Pivot; Investigating; Role; Collagen; BRITE; Pivot; Investigating; Role; Collagen

This Boosting Research Ideas for Transformative and Equitable Advances in Engineering (BRITE) Pivot award supports fundamental research to answer the question of how bone remodels itself into an ideal structure and material. Bone is an extraordinarily ‘smart’ structural material, adapting its composition and properties in response to external forces applied to it. By remodeling itself, bone maintains its mechanically ideal design to support the skeletal system throughout our lifetime. As such, a better understanding of the mechanism to describe its adaptive behavior is extremely useful not only for improving clinical treatment but also for mimicking its design strategy for various engineering applications. This project will employ state-of-the-art techniques matured in the field of micro/nano-technology to develop a multi-scale/multi-physical method specifically designed to address this long-standing biological question. The knowledge obtained from this study will provide strategies to design ‘smart’ materials using piezoelectricity to direct mineralization. The development of such smart materials could improve the safety, effectiveness, and affordability of designs in diverse fields, including bone substitutes, bio-materials, robotics, the automotive industry, clinical treatments, and electronics. The project will support the local community, providing research opportunities for undergraduate students and through the Korean-American society. A mini-conference for high school and early college students will be created as well as specific efforts in Diversity, Equity, and Inclusion (DEI) at the University.This project will focus on discovering bone’s mechano-transduction mechanism using changes in collagen piezo-electricity during the process of mineralization. Collagen I is bone’s main organic constituent and has a pivotal role in providing the structural template for bio-mineralization. However, the underlying mechanism directing the locations of mineral deposition are not known. The project will test the hypothesis that collagen piezo-electricity guides the locations of mineral deposition. The ability to convert mechanical stress into electric charge would therefore be key to targeting the mineral constituents and modulating bone stiffness. The team will design an experimental platform combining an advanced Atomic Force Microscope (AFM) and a micro-electro-mechanical systems (MEMS) loading device to image and biomineralization of collagen under physiological loading to directly observe and investigate in-vitro mineralization correlated with various conditions.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这一促进了工程（BRITE）枢轴奖的变革性和公平进步的研究思想支持基本研究，以回答骨骼如何将自己改造成理想的结构和材料的问题。骨骼是一种非常“智能”的结构材料，可以根据应用于它的外力来调整其成分和特性。通过重塑，Bone保持了机械理想的设计，以支持我们一生中的骨骼系统。因此，对描述其自适应行为的机制有了更好的理解，不仅对于改善临床治疗，而且对于模仿其在各种工程应用的设计策略都非常有用。该项目将采用在微/纳米技术领域成熟的最新技术，以开发一种多规模/多物理方法，专门针对解决这个长期存在的生物学问题。从这项研究中获得的知识将提供使用压电来设计“智能”材料以指导矿化的策略。这种智能材料的开发可以提高潜水场上设计的安全性，有效性和负担能力，包括骨基底物，生物材料，机器人技术，汽车行业，临床处理和电子产品。该项目将支持当地社区，为本科生和韩裔美国社会提供研究机会。将创建对高中和早期大学生的小型会议，以及大学的多样性，公平和包容性（DEI）的特定努力。该项目将着重于在矿化过程中使用胶原压电电力的变化来发现骨骼的机制。胶原蛋白I是骨骼的主要有机构成，在提供生物矿化的结构模板方面具有关键作用。然而，尚不清楚指导矿物沉积位置的基本机制。该项目将检验以下假设：胶原压电电力指导矿物沉积的位置。因此，将机械应力转换为电荷的能力将是靶向矿物一致性和调节骨骼刚度的关键。该团队将设计一个实验平台，该平台结合了先进的原子力显微镜（AFM）和微机械机械系统（MEMS）加载装置，以在生理负载下胶原蛋白的图像和生物矿化，以直接观察和调查维特罗的矿物质，并调查与各种条件相关的，这与NSF的典型范围相关。影响审查标准。