Development of advanced biocatalyst tools and resources to enable biogas-based biomanufacturing.

开发先进的生物催化剂工具和资源，以实现基于沼气的生物制造。

基本信息

批准号：
2225776
负责人：
Calvin Henard
金额：
$ 71.69万
依托单位：
University of North Texas
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-15 至 2025-07-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2225776&HistoricalAwards=false
关键词：
Development advanced biocatalyst tools resources

项目摘要

Biological conversion of methane-rich gas streams, including anaerobic digestion-derived biogas, by methanotrophic bacteria represents a promising route to valorize these abundant, squandered carbon sources while simultaneously mitigating greenhouse gas emissions. This project will develop genetic engineering tools and metabolic models that will advance methanotroph-based biotechnologies aimed at the sequestration/utilization of greenhouse gases as feedstocks for the production of renewable fuels and chemicals. This project will also facilitate the training of underrepresented students at University of North Texas, a Hispanic-serving institution, to promote diversification of the scientific workforce. Further, this project will establish an International Genetically Engineered Machine (iGEM) team consisting of diverse high school, undergraduate, and graduate students that will engage with the broader community to promote synthetic biology and metabolic engineering via scientific outreach activities.The long-term goal of this research is to develop methanotroph-based CH4 and CO2 greenhouse gas mitigation and conversion biotechnologies. To this end, the goals of this project are to 1) onboard Methylococcus capsulatus to the Department of Energy’s Agile Biofoundry and develop genetic tools that enable high-throughput metabolic engineering of this bacterium; 2) map and compare M. capsulatus CH4 and CO2 carbon flux in wild-type and genetically engineered bacteria via 13C fluxomics; and 3) iteratively develop a predictive metabolic model to guide Design-Build-Test-Learn-based metabolic engineering approaches of methanotrophic biocatalysts. Expansion of an advanced genetic engineering toolbox and identification of the coordinated metabolic pathways mediating dual CH4/CO2 utilization and conversion in M. capsulatus will enable the rational metabolic engineering of these organisms for biomanufacturing of green fuels and chemicals from single carbon greenhouse gases.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

甲烷营养细菌对富含甲烷的气流的生物转化，包括厌氧消化的沼气，代表了一种有望在同时减轻温室气体排放的同时重视这些丰富的平方碳源的途径。该项目将开发基因工程工具和代谢模型，这些工具将推动旨在用于隔离/利用温室气体作为用于生产可再生燃料和化学物质的原料的基于甲基营养的生物技术。该项目还将促进西班牙裔服务机构北德克萨斯大学的代表性不足的学生的培训，以促进科学劳动力的多元化。此外，该项目将建立一个由多样性高中，本科生和研究生组成的国际基因工程机器（IGEM）团队，这些团队将通过科学外展活动与更广泛的社区互动，以促进合成生物学和代谢工程。为此，该项目的目标是1）载型甲基甲甲状腺囊肿到能源部的敏捷生物基础，并开发遗传工具，从而使该细菌的高通量代谢工程能够进行高通量的代谢工程； 2）通过13c通量图，并比较了野生型和基因工程细菌中Capsulatus CH4和Co2碳通量； 3）迭代地开发了一种预测代谢模型，以指导基于 - 基于研究的甲烷营养生物催化剂的代谢工程方法。扩展先进的遗传工程工具箱以及对囊膜甲米氏双CH4/CO2利用的协调代谢途径的鉴定，将使这些生物体的合理代谢工程能够为绿色燃料的生物制造进行合理的代谢工程，从而通过自有奖项来促进nsfiration Intervional ofdiffient of Intervistion tinder Intervistion tindure tirument of Intervient of Deem deem deem deem deem deem deem awartor and deem awartiqu and deem awartial deem awartiqu and。和更广泛的影响审查标准。