MRI: Acquisition of a Trapped Ion Mobility Spectrometer Quadrupole Time-of-Flight (TIMS-QTOF) with MALDI for Multidisciplinary Research and Education

MRI：使用 MALDI 获取捕获离子淌度光谱仪四极杆飞行时间 (TIMS-QTOF)，用于多学科研究和教育

• 批准号: 2216089
• 负责人: Yanna Liang
• 金额: $ 109.9万
• 依托单位: SUNY at Albany
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-10-01 至 2025-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2216089&HistoricalAwards=false
• 关键词: MRI; Acquisition; Trapped; Ion; Mobility; MRI; Acquisition; Trapped; Ion; Mobility

This proposal seeks to acquire a state-of-the-art Trapped Ion Mobility Spectrometry (TIMS) Time of Flight (TOF) fleX with Matrix Assisted Laser Desorption/Ionization (MALDI). This instrument offers several unique capabilities: 1) TIM separation allows separating isomeric compounds. These compounds have the same molecular formula, but different chemical properties; 2) TOF enables full mass-spectrum analysis and is able to separate species that differ in mass by as little as 0.001 atomic mass unit; and 3) Mass Spectrometry Imaging is label free, provides untargeted analysis of thousands of molecular species simultaneously, offers extremely low limits of detection, and provides spatial mapping. The capability of this instrument will allow the project team to fill knowledge gaps and tackle pressing challenges in a wide range of scientific fields: environmental monitoring and remediation, biological, chemical, biochemical, and biomedical sciences. Access to this instrument will strengthen research at three Institutions and the Wadsworth Center at the Capital Region of New York and beyond. Besides benefiting the society through new discoveries and deep scientific endeavors, this instrument will be used for educating and training undergraduate and graduate students and outreach to the general public, and K-12 educators and students. The proposed instrument will address critical challenges faced by at least ten researchers in multiple fields of research. This is enabled by the instrument’s revolutionary capabilities in visualizing molecules in chemically complex microenvironments and providing quantitative compositional and structural analyses. These capabilities will bring game-changing discoveries in research related to oral microbial community, dental biofilm, fungal metabolome and proteome, RNA modifications, and epitranscriptome. In environmental impact analysis and remediation, this instrument will be used to detect and quantify isomers of per- and polyfluorinated substances (PFAS) and perform targeted screening and non-target analysis of these compounds in different environmental matrices. In the field of glycomics, the full structural characterization of carbohydrates is a standing analytical need in the characterization of structure-function relationships as well as biomedical applications. The proposed equipment permits confident isomer differentiation and structure elucidation and provides a sensitive and rapid overview of glycan profiles for complex glycoprotein mixtures. With respect to the Drosophila -parasitoid wasp interaction, the immune response of the host fly to another insect, the infecting wasp is largely unknown. The acquired instrument would enable deep understanding of changes at protein levels and localizing the cellular immune response through MALDI imaging.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该提案旨在通过矩阵辅助激光解吸/电离（MALDI）获得最先进的离子移动光谱法（TIMS）飞行时间（TOF）flex。该仪器提供了几种独特的功能：1）TIM分离允许分离异构化合物。这些化合物具有相同的分子式，但化学特性不同。 2）TOF实现了全面的质谱分析，并能够将质量不同的物种分离到0.001原子质量单位； 3）质谱成像不含标签，简单地提供了数千种分子物种的非靶向分析，提供了极低的检测极限，并提供了空间映射。该工具的能力将使项目团队能够填补知识差距，并应对广泛的科学领域的紧迫挑战：环境监测和补救，生物学，化学，化学，生化和生物医学科学。使用该工具将加强纽约首都及其他地区的三个机构和沃兹沃思中心的研究。除了通过新的发现和深刻的科学努力使社会受益外，该乐器还将用于教育和培训本科生和研究生，并向公众推广，以及K-12的教育者和学生。拟议的工具将解决至少十名研究人员在多个研究领域所面临的关键挑战。该仪器在化学复杂的微环境中可视化分子并提供定量组成和结构分析方面的革命性能力可以实现这一点。这些功能将带来与口腔微生物群落，牙科生物膜，真菌代谢组和蛋白质组，RNA修饰和表面翻译组有关的研究中改变游戏的发现。在环境影响分析和修复中，该仪器将用于检测和量化多氟化物质（PFA）的异构体（PFA），并对不同环境物质中这些化合物进行靶向筛选和非目标分析。在糖果学领域，碳氢化物的完整结构表征是结构功能关系以及生物医学应用的表征的常规分析需求。所提出的设备允许自信的异构体分化和结构阐明，并为复杂的糖蛋白混合物提供了敏感和快速的聚糖概况。关于果蝇 - 寄生虫黄蜂相互作用，宿主的免疫响应飞向另一种绝缘，感染的黄蜂在很大程度上是未知的。获得的仪器将能够深入了解蛋白质水平的变化，并通过MALDI成像定位细胞免疫响应。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子和更广泛的影响审查标准，通过评估来诚实地通过评估来诚实地支持。