NSF Postdoctoral Fellowship in Biology: The Effects of Microbiome Ecological and Evolutionary Origin on Plant-Microbiome (Co)Evolution

NSF 生物学博士后奖学金：微生物组生态和进化起源对植物-微生物组（协同）进化的影响

• 批准号: 2209111
• 负责人: Tiffany Batarseh
• 金额: $ 13.8万
• 依托单位: Batarseh, Tiffany N
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2209111&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; Effects

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2021, Integrative Research Investigating the Rules of Life Governing Interactions Between Genomes, Environment and Phenotypes. The fellowship supports research and training of the fellow that will contribute to the area of Rules of Life in innovative ways. The fellow will research the evolution and ecology of microbiomes, which are complex microbial communities critical for proper ecosystem functioning and the health of the complex organisms they often associate with. In agriculture, plant-associated microbiomes have been demonstrated to influence plant health and response to environmental factors. This project will work towards establishing the modes of microbiome evolution and their consequences on plant fitness in order to establish agricultural practices that maintain both plant and microbiome health for greater crop management and food production. Currently, the global demand for food is expected to increase by 35-56% in the next 30 years, highlighting the need for the reevaluation of agricultural practices such that plant-microbiome fitness as well as agricultural productivity and sustainability are maximized. To this end, it is imperative that we understand the factors that influence microbiome evolution and their effects on plant-microbiome interactions, adaptation, and fitness.By leveraging experimental coevolution approaches, this project will determine whether a microbiome’s previous selection environment influences microbial community assembly, function, and adaptive potential when associated with a new plant host. Specifically, the fellow seeks to answer two questions: (1) does a microbial community’s previous associations with heterospecific or conspecific plant hosts influence community adaptation on subsequent hosts?; and (2) what are the consequences of microbiome adaptation to common agricultural chemicals on microbiome function and plant host fitness? To do so, a synthetic microbial community composed of bacteria isolated from the tomato phyllosphere will be evolved on conspecific, heterospecific, or alternating plant hosts with or without common agricultural chemicals before returning to its conspecific host. After coevolution, the genetic and functional changes of the microbial community and the fitness of the host plant will be evaluated to uncover how and when microbiome origin and interaction history will affect microbiome evolution and plant-host fitness. Additionally, the proposed research will allow for training opportunities for undergraduate students through their involvement in experimentation, data analysis, and research presentations.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项行动为2021财年的生物学生物学奖学金提供了一项NSF博士后研究奖学金，综合研究研究了基因组，环境和表型之间相互作用的生活规则。奖学金支持对研究员的研究和培训，这些研究和培训将以创新的方式为生活规则做出贡献。研究员将研究微生物组的进化和生态，它们是复杂的微生物群落，对于适当的生态系统功能和他们经常与之相关的复杂生物的健康至关重要。在同意时，已经证明与植物相关的微生物组影响植物健康和对环境因素的反应。该项目将致力于建立微生物组进化的模式及其对植物健身的后果，以建立维持植物和微生物组健康的农业实践，从而为更多的农作物管理和食品生产。目前，在未来30年内，全球对粮食的需求预计将增长35-56％，强调需要重新评估农业实践，从而最大程度地提高了植物 - 微生物组以及农业生产力和可持续性。为此，我们必须了解影响微生物组进化的因素及其对植物 - 微生物组相互作用，适应性和适应性的影响。通过利用实验性协同进化方法，该项目将确定微生物组的先前选择环境会影响微生物组社区组装，功能，以及与新植物宿主相关时的适应性潜力。具体来说，该研究员试图回答两个问题：（1）微生物组社区以前与异种或特定植物寄主的协会是否会影响社区对随后的宿主的适应？ （2）微生物组适应普通农业化学物质对微生物组功能和植物宿主健身的后果是什么？为此，由从番茄植物层分离的细菌组成的合成微生物群落将在返回其特定宿主之前具有或没有常见的农业化学物质的特定，异种或替代植物宿主进化。协同进化后，将评估微生物群落的遗传和功能变化以及宿主植物的适应性，以发现微生物组的起源和相互作用史将影响微生物组的演变和植物主 - 宿主健身。此外，拟议的研究将通过参与实验，数据分析和研究演讲为本科生提供培训机会。该奖项反映了NSF的法定任务，并通过评估该基金会的知识分子优点和更广泛的影响来审查标准。