Diamond-based Platforms for Long-term Growth and Investigations of Neurons

用于神经元长期生长和研究的金刚石平台

• 批准号: 251234513
• 负责人: Professor Dr. Cyril Popov
• 金额: --
• 依托单位: European Commission
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/251234513?language=en
• 关键词: Diamond; based; Platforms; Long; term

The recent development of neurosciences creates an ever increasing demand to the properties and functionalities of materials and devices used for the study of fully organized neuronal networks and their activity. They should provide good and fast attachment of the neurons without the application of extracellular matrix proteins and should not degrade with the time upon contact with the biological system thus allowing long-term measurements. Diamond in the form of thin films is a perspective candidate for such applications due to its outstanding mechanical, optical, electrical, chemical, and bio-properties. This research proposal aims at the preparation of platforms based on ultrananocrystalline diamond (UNCD) films for the long-term growth of functional neuronal networks and their electrophysiological investigations. The UNCD films which are composed of diamond nanocrystallites (ca. 5 nm) embedded in amorphous carbon matrix combine the extreme diamond properties with a rather smooth topography and include nanostructures which will enhance the fast and good adhesion of the neurons. During this work multilayers of ultrananocrystalline diamond and metal structures will be deposited on glass substrates with a transparency allowing the microscopical observation of the neuron positioned on the platforms from the backside combined with, for example, fluorescence imaging from the top. As a next step, from these layers the metal tracks for recording the neuron activity and the UNCD for the contact pads for the neurons will be structured applying lithography and etching. Since the surface plays an extremely important role in the interactions with cells various modifications of the UNCD surface by plasma or photochemical processes will be applied, followed by patterning of the surface termination. For the investigation of the biocompatibility and functionality of the layer systems two types of neurons will be used, namely pacemaker neurons of the cockroach Rhypharobia maderae and olfactory receptor neurons from the tobacco hornworm Manduca sexta which serve as models for the investigation of the spontaneous electrical activity and the directional growth of developing neuronal networks, respectively, both aiming at long-term studies, not achieved till now with other materials.

神经科学的最新发展对用于研究完全有组织的神经元网络及其活性的材料和设备的性质和功能的需求不断增长。它们应在不应用细胞外基质蛋白的情况下提供神经元的良好和快速的附着，并且不应随着与生物系统接触时的时间降解，从而可以长期测量。由于其出色的机械，光学，电气，化学和生物份量，薄膜形式的钻石是薄膜的形式的候选者。该研究建议旨在制备基于Ultranocrystalline Diamond（UNCD）膜的平台，用于功能性神经元网络的长期增长及其电生理研究。由嵌入无定形碳基质中的钻石纳米晶（Ca. 5 nm）组成的UNCD膜将极端钻石的特性与相当光滑的地形结合在一起，并包括纳米结构，并包括可以增强神经元快速和良好粘附的纳米结构。在这项工作期间，超级晶体钻石和金属结构的多层将沉积在玻璃基板上，具有透明度，从而使对位于背面平台上的神经元的显微镜观察结合，例如，荧光成像从顶部进行。下一步，从这些层中，用于记录神经元活性的金属轨道和神经元的接触板的UNCD将进行构建，以应用光刻和蚀刻。由于表面在与细胞的相互作用中起着极为重要的作用，将应用血浆或光化学过程的各种修饰，然后将表面终止构图。 For the investigation of the biocompatibility and functionality of the layer systems two types of neurons will be used, namely pacemaker neurons of the cockroach Rhypharobia maderae and olfactory receptor neurons from the tobacco hornworm Manduca sexta which serve as models for the investigation of the spontaneous electrical activity and the directional growth of developing neuronal networks, respectively, both aiming at long-term studies, not到目前为止使用其他材料实现。

项目成果

Plasma surface fluorination of ultrananocrystalline diamond films

• DOI: 10.1016/j.surfcoat.2016.06.029
• 发表时间: 2016-09
• 期刊: Surface & Coatings Technology
• 影响因子: 5.4
• 作者: W. Kulisch;A. Voss;D. Merker;J. Reithmaier;R. Merz;M. Kopnarski;C. Popov

Strong attachment of circadian pacemaker neurons on modified ultrananocrystalline diamond surfaces.

• DOI: 10.1016/j.msec.2016.03.092
• 发表时间: 2016-07
• 期刊: Materials science & engineering. C, Materials for biological applications
• 作者: A. Voss;Hongying Wei;Yi Zhang;S. Turner;G. Ceccone;J. Reithmaier;M. Stengl;C. Popov