NSF Postdoctoral Fellowship in Biology FY 2021: Using butterfly wing color patterns to elucidate an uncharacterized Wnt pathway

2021 财年 NSF 生物学博士后奖学金：利用蝴蝶翅膀颜色模式阐明未表征的 Wnt 通路

基本信息

批准号：
2109536
负责人：
Anyimilehidi Mazo-Vargas
金额：
$ 13.8万
依托单位：
Mazo-Vargas, Anyimilehidi
依托单位国家：
美国
项目类别：
Fellowship Award
财政年份：
2022
资助国家：
美国
起止时间：
2022-03-01 至 2024-02-29
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2109536&HistoricalAwards=false
关键词：
NSF Postdoctoral Fellowship Biology FY

项目摘要

This action funds an NSF Postdoctoral Research Fellowship in Biology for FY 2021, Broadening Participation of Groups Under-represented in Biology. The Fellowship supports a research and training plan for the Fellow that will increase the participation of groups underrepresented in biology. Multicellular animals share common signaling pathways that are responsible for patterning and regulating development in early animals (embryos) all the way to adults. One such pathway is called the Wnt signaling pathway, and its study has been instrumental to our understanding of cell-cell communication, animal development, stem cell biology, and cancer. Wnt pathways have been well studied, and a “canonical pathway” is the best understood. However, there are non-canonical functions of the Wnt pathway as well. This research aims to integrate genomic tools and the attributes of butterfly wing patterns to determine how Wnt signals instruct pattern formation in these wings. Furthermore, the fellow will take advantage of butterfly biology's natural appeal to increase the participation of underrepresented groups in life sciences. Specifically, (1) creating a Spanish-language active learning module for bilingual schools, (2) providing summer internships to two high-school students per year with the sponsoring scientist Dr. Arnaud Martin. (3) Encouraging the participation of diverse scientists at the postdoctoral level.Butterfly wing patterns are visually compelling, two-dimensional arrays of differentiated cells where signaling outputs from the tissue are directly translated into color patterns. Preliminary data from the fellow's Ph.D. work and the sponsoring lab proposes that the conserved WntA molecule organizes discrete domains of color-forming scale cells, potentially independently of the canonical Wnt pathway. Hence, the fellow will generate a laboratory colony of the butterfly Junonia coenia deficient for the WntA protein; then, she will survey how WntA presence/absence impacts gene expression and chromatin accessibility during wing development. Those data will provide a genome-wide landscape of the cellular responses to WntA signaling, which the fellow will use to select candidate genes for specific assays:visualizing mRNA and protein in the developing wings (i.e., immunochemistry), and CRISPR/cas9 gene knock-outs. The fellow will formally test the non-canonicity of WntA signaling and tackle the molecular mechanisms that mediate signal integration and result in sharp cell specification domains, as observed in the sharp boundaries that delineate color patterns. This original work will be important for a comprehensive understanding of Wnt signaling and may shed light on novel aspects of the pathway that could extend across the animal kingdom. In this proposal, the fellow will work with outreach programs, training and mentoring of future scientists, and develop a networking group for Latin-American and first-generation graduate students and postdocs interested in EvoDevo.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该行动资助了 2021 财年 NSF 生物学博士后研究奖学金，扩大生物学领域代表性不足的群体的参与。该奖学金支持该研究员的研究和培训计划，该计划将增加生物学领域代表性不足的群体的参与。负责从早期动物（胚胎）一直到成年的发育模式和调节的信号通路，其中一种通路称为 Wnt 信号通路，它的研究有助于我们理解这一通路。细胞间通讯、动物发育、干细胞生物学和癌症已经得到了充分的研究，并且“规范途径”是最好的理解，然而，这项研究也存在非规范功能。旨在整合基因组工具和蝴蝶翅膀图案的属性，以确定 Wnt 信号如何指导这些翅膀的图案形成。此外，该研究员将利用蝴蝶生物学的自然吸引力来增加代表性不足的群体对生命科学的参与。 1）为双语学校创建西班牙语主动学习模块，(2) 每年为两名高中生提供赞助科学家阿诺·马丁博士的暑期实习机会，(3) 鼓励博士后水平的多元化科学家的参与。蝴蝶。翅膀图案是视觉上引人注目的分化细胞的二维阵列，其中来自组织的信号输出直接转化为颜色图案。该研究员的博士工作和资助实验室的初步数据表明。 WntA 分子组织颜色形成鳞片细胞的离散域，可能独立于规范的 Wnt 通路，因此，该研究员将生成一个缺乏 WntA 蛋白的蝴蝶 Junonia coenia 的实验室群体，然后她将调查 WntA 的存在/缺失。这些数据将提供细胞对 WntA 信号反应的全基因组图谱，研究员将利用它来选择用于特定分析的候选基因：可视化。翅膀中的 mRNA 和蛋白质（即免疫化学），以及 CRISPR/cas9 基因敲除，该研究员将正式测试 WntA 信号传导的非规范性，并解决开发介导信号整合并导致尖锐细胞规范域的分子机制。正如在描绘颜色模式的清晰边界中所观察到的那样，这项原创工作对于全面理解 Wnt 信号传导非常重要，并可能揭示可能延伸到动物王国的途径的新方面。工作通过外展计划、对未来科学家的培训和指导，并为对 EvoDevo 感兴趣的拉丁美洲和第一代研究生和博士后建立一个网络小组。该奖项的法定使命，并通过使用基金会的智力价值进行评估，被认为值得支持以及更广泛的影响审查标准。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Frizzled2 receives WntA signaling during butterfly wing pattern formation

Frizzled2 在蝴蝶翅膀图案形成过程中接收 WntA 信号

DOI：
10.1242/dev.201868
发表时间：
2023-08
期刊：
Development
影响因子：
4.6
作者：
Hanly, Joseph J.;Loh, Ling S.;Mazo;Rivera;Livraghi, Luca;Tendolkar, Amruta;Day, Christopher R.;Liutikaite, Neringa;Earls, Emily A.;Corning, Olaf B. W. H.;et al
通讯作者：
et al

Deep cis-regulatory homology of the butterfly wing pattern ground plan

蝴蝶翅膀图案平面图的深度顺式调控同源性

DOI：
10.1126/science.abi9407
发表时间：
2022-10
期刊：
Science
影响因子：
56.9
作者：
Mazo;Langmüller, Anna M.;Wilder, Alexis;van der Burg, Karin R.;Lewis, James J.;Messer, Philipp W.;Zhang, Linlin;Martin, Arnaud;Reed, Robert D.
通讯作者：
Reed, Robert D.

Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths

Plodia 蛾隐性白眼突变的定位和 CRISPR 同源定向修复

DOI：
10.1016/j.isci.2022.103885
发表时间：
2022-03-18
期刊：
iScience
影响因子：
5.8
作者：
Heryanto C;Hanly JJ;Mazo-Vargas A;Tendolkar A;Martin A
通讯作者：
Martin A

Cis-regulatory modes of Ultrabithorax inactivation in butterfly forewings

蝴蝶前翅 Ultrabithorax 失活的顺式调节模式

DOI：
10.1101/2023.08.18.553910
发表时间：
2023-10-05
期刊：
eLife
影响因子：
7.7
作者：
A. Tendolkar;Anyi Mazo;L. Livraghi;Joseph J. Hanly;Kelsey C. Van Horne;L. Gilbert;Arnaud Martin
通讯作者：
Arnaud Martin

Efficient hyperactive piggyBac transgenesis in Plodia pantry moths

Plodia 食品储藏室蛾中高效的过度活跃的piggyBac转基因