Elucidating Fundamental Factors Driving Self-assembly with Guided Interactions in Multicomponent Enzyme Systems Using Model Nanostructured Platforms

使用模型纳米结构平台阐明多组分酶系统中通过引导相互作用驱动自组装的基本因素

基本信息

批准号：
2108448
负责人：
Cindy Berrie
金额：
$ 50.85万
依托单位：
University of Kansas Center for Research Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2025-06-30
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2108448&HistoricalAwards=false
关键词：
Elucidating Fundamental Factors Driving Self

项目摘要

With the support of the Macromolecular, Supramolecular and Nanochemistry program in the Division of Chemistry and the Established Program to Stimulate Competitive Research (EPSCoR), Professors Cindy L. Berrie and Candan Tamerler at the University of Kansas are investigating factors that govern the assembly and organization of biomolecules at interfaces. Affinity peptide tags will be used to selectively direct the self-assembly of biomolecules, including enzymes, onto material surfaces to create multicomponent bioactive materials organized at the nanoscale. The metal nanostructure platforms being developed are designed to enable an understanding of the role of material specificity, curvature, spacing, and size on the spatially organized self-assembly of biomolecules. The project will allow biohybrid materials to mimic the exquisite functionality nature has evolved for complex tasks, which will enable enhanced biosensing, biocatalysis and biofuel applications as an alternative energy source. In the course of conducting the project, graduate and undergraduate students will be trained in the growing convergence of nanoscience, biomolecules and biomaterials. In addition, the research team will carry out outreach and services to the community at the University of Kansas and local middle and elementary schools through participation in the Engineering EXPO and the Carnival of Chemistry events and the development of the “Science Night” program to engage students in science at an early stage. Public demonstrations on nanolithography and imaging will be conducted with the involvement of the students working on the project. The project focuses on exploring the fundamental factors responsible for peptide guided self-assembly of multi-enzyme systems using model nanostructured platforms to harness their coordinated activity. Nature exquisitely organizes cascades of enzymes to work in tandem; however, attempts to artificially assemble such complex systems are hampered by the complexity and lack of information about the factors governing functional assembly. Emerging applications from biocatalysis to biosensing, to energy harvesting and biofuels would likely benefit from assembly of coupled enzymes with cascade-like activity, and therefore elucidating the factors controlling the assembly of such complex systems would have wide ranging applications. Specifically, the assembly of peptide tags, peptide-labeled enzymes, and the co-assembly of coupled enzyme pairs will be investigated using optical and atomic force microscopy as well as bioactivity assays to determine the distribution, conformation, and orientation of assembled biomolecules and how these are affected by the metal nanostructure composition, spacing, size, and curvature. The scientific broader impacts of the work include the development of design principles for biohybrid materials for applications in biosensing and biocatalysis. There are also important elements of workforce development in the area of nanobiomaterials and of outreach the community.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系高分子、超分子和纳米化学项目以及刺激竞争性研究既定项目 (EPSCoR) 的支持下，堪萨斯大学教授 Cindy L. Berrie 和 Candan Tamerler 正在研究控制组装和组织的因素亲和肽标签将用于选择性地引导生物分子（包括酶）在材料表面上进行自组装。创建纳米级组织的多组分生物活性材料。正在开发的金属纳米结构平台旨在帮助了解材料特异性、曲率、间距和尺寸对生物分子空间组织自组装的作用。该项目将允许生物混合材料。模仿大自然为复杂任务而进化的精致功能，这将增强生物传感、生物催化和生物燃料作为替代能源的应用。在该项目的过程中，研究生和本科生将接受培训。此外，研究团队还将通过参加工程博览会和化学嘉年华活动，向堪萨斯大学和当地中小学的社区进行推广和服务。开发“科学之夜”计划，让学生尽早参与科学，将在参与该项目的学生的参与下进行公开演示，该项目的重点是探索肽的基本因素。使用模型纳米结构平台引导多酶系统自组装，以利用其协调活动。然而，人工组装此类复杂系统的尝试因复杂性和缺乏相关信息而受到阻碍。从生物催化到生物传感、能量收集和生物燃料的新兴应用可能会受益于具有级联活性的偶联酶的组装，从而阐明这些因素。控制此类复杂系统的组装将具有广泛的应用，具体来说，将使用光学和原子力显微镜以及生物活性测定来研究肽标签、肽标记酶的组装以及偶联酶对的共组装。确定组装生物分子的分布、构象和方向，以及它们如何受到金属纳米结构组成、间距、尺寸和曲率的影响。这项工作的科学影响包括生物混合设计原理的更广泛发展。纳米生物材料领域的劳动力发展和社区推广也是重要的内容。该奖项是 NSF 的法定使命，通过使用基金会的智力优势和更广泛的评估，被认为值得支持。影响审查标准。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Spatiotemporal Imaging of Zinc Ions in Zebrafish Live Brain Tissue Enabled by Fluorescent Bionanoprobes

荧光生物纳米探针实现斑马鱼活体脑组织中锌离子的时空成像

DOI：
10.3390/molecules28052260
发表时间：
2023-02-28
期刊：
Molecules
影响因子：
4.6
作者：
Jarosova R;Woolfolk SK;Martinez-Rivera N;Jaeschke MW;Rosa-Molinar E;Tamerler C;Johnson MA
通讯作者：
Johnson MA

Atomic Force Microscopy-Based Static Plowing Lithography Using CaCO 3 Nanoparticle Resist Layers as a Substrate-Flexible Selective Metal Deposition Resist

基于原子力显微镜的静态犁耕光刻，使用 CaCO 3 纳米粒子抗蚀剂层作为基板-柔性选择性金属沉积抗蚀剂

DOI：
10.1021/acs.jpcc.1c07239
发表时间：
2021-10
期刊：
The Journal of Physical Chemistry C
影响因子：
0
作者：
Ulapane, Sasanka B.;Doolin, Jennifer L.;Okeowo, Monisola K.;Berrie, Cindy L.
通讯作者：
Berrie, Cindy L.

Solid-Binding Peptide-Guided Spatially Directed Immobilization of Kinetically Matched Enzyme Cascades in Membrane Nanoreactors

膜纳米反应器中固体结合肽引导的动力学匹配酶级联的空间定向固定