EFRI E3P: Program plastic lifecycle by rationally design enzyme-containing plastics

EFRI E3P：通过合理设计含酶塑料来规划塑料生命周期

• 批准号: 2132025
• 负责人: Ting Xu
• 金额: $ 200万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2132025&HistoricalAwards=false
• 关键词: EFRI; E3P; Program; plastic; lifecycle

This project targets embedding active enzymes during plastic manufacturing, and programming on-demand plastic degradation or modification during product fabrication, storage, and use. The project's hypotheses are that (1) molecular control of biocatalysis within polymeric matrices can afford on-demand modification or programmable plastic degradation during manufacture, use, and disposal, and (2) with recent advances in synthetic biology and nanomaterial engineering, it is feasible to develop economically viable enzyme-embedded plastics to achieve valorization or degradation of end-of-life plastic waste. The interdisciplinary project team seeks to develop compostable single-use plastic based on polymers with chemically labile backbones and valorization of polyolefins by C-H bond oxidation. The team intends to (1) develop approaches to manufacturing these materials at large scale using melt extrusion, (2) test properties of prototypes, and (3) evaluate the environmental impact of the prototypes with local ecology centers. By engineering compostable plastics that are stable under conditions relevant to storage and use but degrade in a matter of days under composting conditions, this project seeks to provide basic knowledge of important relevance to the compostable single-use plastics industry. The intended outcome of the research would reduce the volume of single-use plastic packaging sent to landfills and reduce mechanical separation costs at organics processing and composting facilities. The research plan is to (1) develop molecular understanding in biocatalysis of enzymes nanoscopically dispersed in semi- crystalline polyesters with focus on enzyme stability, catalytic mechanism, and kinetics to realize programmable degradation, (2) develop molecular understanding in biocatalysis in oxidase-containing polyolefins and design catalytic cascades to chemically modify sidechains of polyolefins for valorization and accelerated degradation, and (3) fabricate minimum viable prototypes, optimize the system for relevant commercial performance as single-use plastics, validate the material properties and performance using ASTM testing methodology, and perform technoeconomic analysis and evaluate prototype life cycle. It is anticipated that the work will provide significant insights in the emerging fields of solid-state enzymology and enzyme-containing functional materials. The project team also plans a three-tiered approach aimed at improving undergraduate education in the area of polymer science, developing a polymer degradation kit for students and public demonstrations, and creating course modules to specifically highlight polymer recycling, upgrading, and enzymatic degradation. This award is co-funded by the directorate of Mathematical and Physical Sciences, specifically the Polymers Program in the Division of Materials Research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目靶向在塑料制造过程中嵌入活性酶，以及在产品制造，存储和使用过程中按需塑料降解或修改的编程。该项目的假设是（1）在制造，使用和处置过程中，聚合物矩阵内对生物催化的分子控制可以提供按需修改或可编程的塑料降解，以及（2）在合成生物学和纳米材料工程中的最新进步，可以在经济上可行的塑料工程，以实现经济化的塑料范围，以实现跨越的塑料范围，以实现经济化的范围内，以实现型号的层次范围内的室内，以供室内的室内居住的层面型居住在室内。 浪费。跨学科项目团队旨在根据C-H键氧化的聚合物和聚烯烃对聚合物的聚合物进行基于聚合物的塑性塑料。该团队打算（1）使用熔体挤出，（2）原型测试特性，以及（3）评估原型与当地生态中心的环境影响。通过工程堆肥塑料在与存储和使用相关的条件下稳定但在堆肥条件下在几天内降解，该项目旨在提供与可堆肥的一次性塑料行业重要相关的基本知识。研究的预期结果将减少发送给垃圾填埋场的一次性塑料包装的量，并降低有机物处理和堆肥设施中的机械分离成本。研究计划是（1）在半结晶多植物中分散纳米酶的生物催化中的分子理解，侧重于酶稳定性，催化机制和动力学，以实现可编程性降解，以在氧化酶的氧化酶氧化量和设计中，在氧化酶的氧化酶中发展氧化酶的分子理解。多烯烃用于价值和加速降解，以及（3）制造最小可行的原型，优化系统，将相关的商业性能作为单使用塑料，使用ASTM测试方法验证材料特性和性能，并执行技术经济学分析和评估原型生命周期。可以预计，这项工作将在固态酶学和含酶的功能材料的新兴领域提供重要的见解。该项目团队还计划采用三层方法，旨在改善聚合物科学领域的本科教育，为学生和公众示威开发聚合物降解套件，并创建课程模块以特别突出聚合物的回收，升级，升级，并酶促降解。该奖项由数学和物理科学局共同资助，特别是材料研究部的聚合物计划。该奖项反映了NSF的法定任务，并被认为是通过基金会的知识分子和更广泛影响的评估审查标准的评估来通过评估来支持的。