Collaborative Research: Microbiota Role in Intestinal Regeneration

合作研究：微生物群在肠道再生中的作用

• 批准号: 2100494
• 负责人: Jose Garcia-Arraras
• 金额: $ 66.12万
• 依托单位: University of Puerto Rico-Rio Piedras
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2100494&HistoricalAwards=false
• 关键词: Collaborative; Research; Microbiota; Role; Intestinal

Little is known about why some animals can regenerate certain tissues or organs, while other species cannot. This research explores regeneration in a novel organism, the sea cucumber Holothuria glaberrima. These animals have an incredible ability to regenerate lost structures and this study will focus on the regeneration of the intestine. In recent years, the microbial environment associated with organisms, or microbiota, has been shown to play important roles in the development and proper function of an organism. This project aims to determine the possible role of the microbiota on the process of intestinal regeneration. For this study, the bacteria and other microorganisms that are associated with the regenerating intestine will be characterized to determine if and which species might be modulating intestinal regeneration. Moreover, the project provides "hands-on" research training to undergraduate students as a way of guiding them toward scientific research careers and help increase the diversity of the scientific community. The microbiota within the digestive tract has been shown to modulate energy metabolism and immunity in both vertebrate and invertebrate species. However, little is known of their role in organogenesis and in particular in the regeneration of new organs following loss or injury. This proposal aims to extend our knowledge of the microbiota role in developmental and regenerative processes. The proposal brings together two research scientists with different areas of expertise that will join forces to focus on the characterization of holothurian intestinal microbiota and its function in the regeneration of the holothurian digestive tract. To determine the possible effects of the microbiota, state of the art technology including RNA-Seq and 16rRNA sequencing together with more traditional microbiota analyses will be utilized. The proposed project will: (1) Characterize the intestinal microbiota of two different species of holothurians, that inhabit different environments and use different feeding methods and determine the spatial and temporal changes in the microbiota during intestinal regeneration. (2) Determine the role of the microbiota by modifying the microbiota using antibiotics and antifungal agents. (3) Identify the host genes that are under the control of the microbiota in normal and regenerating animals by performing transcriptomic analyses of normal and regenerating animals treated with specific antibiotic agents. The outcome of the proposal will be a clear description of how the microbiota modulates intestinal regeneration, a characterization of species that play a major role in the process and the target genes involved in the process.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

关于某些动物为什么可以再生某些组织或器官而其他物种不能再生的原因知之甚少。这项研究探索了一种新型生物体的再生，即海参霍洛氏菌·格拉贝里玛。这些动物具有令人难以置信的再生结构的能力，这项研究将集中于肠的再生。 近年来，与生物体或微生物群有关的微生物环境已被证明在生物体的发育和正常功能中起着重要作用。该项目旨在确定微生物群在肠再生过程中的可能作用。在这项研究中，与肠道再生有关的细菌和其他微生物的特征是确定是否以及哪种物种可能正在调节肠道再生。此外，该项目为本科生提供了“动手”研究培训，以指导他们从事科学研究职业并帮助增加科学界的多样性。消化道内的微生物群已被证明可以调节脊椎动物和无脊椎动物物种的能量代谢和免疫力。然而，鲜为人知的作用在器官发生中，尤其是在损失或受伤后新器官的再生中的作用。该建议旨在扩展我们对微生物群在发展和再生过程中的作用的了解。该提案汇集了两名具有不同专业知识领域的研究科学家，它们将联合起来，专注于Holothurian肠道微生物群的表征及其在Holothurian消化道再生中的功能。为了确定微生物群的可能影响，将利用包括RNA-Seq和16RRNA测序在内的最先进的技术以及更传统的微生物群分析。拟议的项目将：（1）表征两种不同种类的植物群的肠菌群，它们栖息在不同的环境中，并使用不同的喂养方法，并确定肠再生过程中微生物群的空间和时间变化。 （2）通过使用抗生素和抗真菌剂修饰微生物群来确定菌群的作用。 （3）通过对用特定抗生素治疗的正常和再生动物进行转录组分析，确定正常和再生动物中微生物群控制的宿主基因。该提案的结果将清楚地描述微生物群如何调节肠再生，这是该过程中主要作用的物种的特征以及该过程中涉及的目标基因。该奖项反映了NSF的法定任务，并被认为是通过该基金会的知识分子和宽广的影响来评估的支持，并被认为是值得的。