In vivo tumor imaging with para-hydrogen

使用仲氢进行体内肿瘤成像

• 批准号: 245777188
• 负责人: Professor Dr. Bernhard Blümich
• 金额: --
• 依托单位: Institut für Experimentelle Molekulare Bildgebung (ExMI)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/245777188?language=en
• 关键词: vivo; tumor; imaging; para; hydrogen

The main goal of the proposed project is the development of an inexpensive alternative to investigate molecular processes in tumors, which shows great potential for clinical applications because of its cost efficiency and its easiness to handle. This goal will be achieved by combining easy processes of hyperpolarizing amino acid derivatives with magnetic resonance tomography (MRT).In the course of the project magnetic resonance studies of amino acids and small peptides (NGR, cRGD), which will be hyperpolarized by Para-Hydrogen Induced Polarization (PHIP) and especially with the Signal Amplification by Reversible Exchange-method (SABRE), will be undertaken. The second part of the project will focus on in-vitro studies using flow cells. Carcinoma cells will be overflowed with hyperpolarized tracers and signal-time-curves will be recorded with dynamic MRT measurements and evaluated pharmacokinetically. The third part of the project contains in-vivo experiments with tumor-bearing rats to investigate the potential to analyze molecular processes in tumors with MRT.

该项目的主要目标是开发一种廉价的替代方案来研究肿瘤中的分子过程，由于其成本效益和易于处理，该方案在临床应用方面显示出巨大的潜力。这一目标将通过将超极化氨基酸衍生物的简单过程与磁共振断层扫描（MRT）相结合来实现。在该项目的过程中，对氨基酸和小肽（NGR，cRGD）进行磁共振研究，这些肽将通过对位超极化将进行氢致极化（PHIP），特别是可逆交换信号放大方法（SABRE）。该项目的第二部分将重点关注使用流动池的体外研究。癌细胞将充满超极化示踪剂，并且将通过动态 MRT 测量记录信号时间曲线并进行药代动力学评估。该项目的第三部分包含对荷瘤大鼠进行体内实验，以研究利用 MRT 分析肿瘤分子过程的潜力。

项目成果

Direct Hyperpolarization of Nitrogen-15 in Aqueous Media with Parahydrogen in Reversible Exchange.

• DOI: 10.1021/jacs.7b00569
• 发表时间: 2017-06-14
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Colell JFP;Emondts M;Logan AWJ;Shen K;Bae J;Shchepin RV;Ortiz GX Jr;Spannring P;Wang Q;Malcolmson SJ;Chekmenev EY;Feiters MC;Rutjes FPJT;Blümich B;Theis T;Warren WS
• 通讯作者: Warren WS

Optimierungen von Hyperpolarisationsmethoden basierend auf para-Wasserstoff hinsichtlich ihrer Biokompatibilität für in-vivo-Anwendungen in der Kernspinresonanz

基于仲氢的超极化方法在核磁共振体内应用生物相容性方面的优化

• DOI: 10.18154/rwth-2017-09577
• 发表时间: 2017
• 作者: Meike Emondts

Status and trends in the development of clinical diagnostic agents.

• DOI: 10.1002/wnan.1441
• 发表时间: 2017-07
• 期刊: Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
• 作者: Tatjana Opacic;V. Paefgen;T. Lammers;F. Kiessling