MRI: Acquisition of a Hybrid Quadrupole Orbitrap LC/MS System to Facilitate Trace Quantitation and Complex Mixture Analyses at the University of Alaska Anchorage

MRI：阿拉斯加大学安克雷奇分校购买混合四极 Orbitrap LC/MS 系统以促进痕量定量和复杂混合物分析

• 批准号: 2019123
• 负责人: Patrick Tomco
• 金额: $ 45万
• 依托单位: University of Alaska Anchorage Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019123&HistoricalAwards=false
• 关键词: MRI; Acquisition; Hybrid; Quadrupole; Orbitrap

This award is supported by the Major Research Instrumentation, the Chemistry Research Instrumentation programs and the Established Program to Stimulate Competitive Research (EPSCoR). The University of Alaska-Anchorage is acquiring a high-performance benchtop quadrupole system that combines a quadrupole, an ion routing multipole and an Orbitrap analyzer and a liquid to support Professor Patrick Tomco, Jason Burkhead, Aaron Dotson, Colin McGill, and other colleagues. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In a quadrupole mass spectrometer, there are four cylindrical rods parallel to each other, that serve as the mass analyzer (the component of the instrument responsible for selecting sample ions based on their mass-to-charge ratio (m/z)). Ions are separated in a quadrupole based on the stability of their trajectories in oscillating electric fields that are applied to the rods. In this instrument, this capability is coupled to an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle. The image from the trapped ions is detected and converted to a mass spectrum using the Fourier transform of the frequency signal. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used in outreach activities involving the University of Alaska-Fairbanks (UAF) and the University of Alaska-Southeast (UAS) as well as agencies such as the Alaska Department of Fish & Game and the Alaska State Crime Lab and collaborators from the University of New Orleans.The award of this mass spectrometer is aimed at enhancing research and education at all levels across the University of Alaska system. It especially impacts understanding the fate of the insecticide rotenone isoflavone in the changing climate of southcentral Alaska. The instrumentation is also used for exploring environmental oil spill damage mitigation in the Artic and Sub-artic and for the study of metabolomics. In addition, it contributes to the analysis of the impact of climate change on plant-herbivore dynamics in the Artic and Boreal ecoregions, and the study of medicinal properties of Alaskan botanicals. The mass spectrometer is also used to understand essential amino acid metabolism in the microbiomes of hibernatic Artic ground squirrels using 15-nitrogen-labeled nitrogen sources.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要研究仪器、化学研究仪器项目和刺激竞争性研究既定项目 (EPSCoR) 的支持。阿拉斯加大学安克雷奇分校正在采购一套高性能台式四极杆系统，该系统结合了四极杆、离子路由多极杆和 Orbitrap 分析仪以及液体，以支持 Patrick Tomco 教授、Jason Burkhead、Aaron Dotson、Colin McGill 和其他同事。一般来说，质谱 (MS) 是用于识别和表征复杂样品中少量化学物质的关键分析方法之一。带有液相色谱仪的仪器可以在化合物混合物到达质谱仪之前对其进行分离。在四极杆质谱仪中，有四个彼此平行的圆柱形杆，用作质量分析器（仪器的组件，负责根据质荷比 (m/z) 选择样品离子）。离子在四极杆中根据其在施加到杆上的振荡电场中的轨迹的稳定性进行分离。在该仪器中，此功能与离子阱质量分析器耦合，该离子阱质量分析器由外部桶状电极和同轴内部纺锤状电极组成，该电极捕获绕纺锤轨道运动的离子。使用频率信号的傅立叶变换检测捕获离子的图像并将其转换为质谱。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与这种现代分析技术来扩大参与范围。它还用于涉及阿拉斯加大学费尔班克斯分校 (UAF) 和阿拉斯加东南大学 (UAS) 以及阿拉斯加渔猎部和阿拉斯加州立犯罪实验室等机构以及来自阿拉斯加大学的合作者的外展活动。新奥尔良大学。该质谱仪的获奖旨在加强阿拉斯加大学系统各个级别的研究和教育。它尤其影响了解杀虫剂鱼藤酮异黄酮在阿拉斯加中南部不断变化的气候中的命运。该仪器还用于探索北极和亚北极环境石油泄漏损害的减轻以及代谢组学的研究。此外，它还有助于分析气候变化对北极和北方生态区植物-草食动物动态的影响，以及阿拉斯加植物药用特性的研究。该质谱仪还用于使用 15 氮标记的氮源来了解冬眠北极地松鼠微生物组中的必需氨基酸代谢。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优点和技术进行评估，认为值得支持。更广泛的影响审查标准。

项目成果

Photochemical formation of water-soluble oxyPAHs, naphthenic acids, and other hydrocarbon oxidation products from Cook Inlet, Alaska crude oil and diesel in simulated seawater spills

模拟海水泄漏中库克湾、阿拉斯加原油和柴油中水溶性多环芳烃、环烷酸和其他碳氢化合物氧化产物的光化学形成

• DOI: 10.1039/d2va00325b
• 发表时间: 2023-03
• 期刊: Environmental Science: Advances
• 作者: Harsha, Maxwell L.;Redman, Zachary C.;Wesolowski, Josh;Podgorski, David C.;Tomco, Patrick L.
• 通讯作者: Tomco, Patrick L.

Reactive Oxygen Species and Chromophoric Dissolved Organic Matter Drive the Aquatic Photochemical Pathways and Photoproducts of 6PPD-quinone under Simulated High-Latitude Conditions

模拟高纬度条件下活性氧和发色溶解有机物驱动 6PPD-醌的水生光化学途径和光产物

• DOI: 10.1021/acs.est.3c05742
• 发表时间: 2023-12
• 期刊: Environmental Science & Technology
• 影响因子: 11.4
• 作者: Redman, Zachary C.;Begley, Jessica L.;Hillestad, Isabel;DiMento, Brian P.;Stanton, Ryan S.;Aguaa, Alon R.;Pirrung, Michael C.;Tomco, Patrick L.
• 通讯作者: Tomco, Patrick L.