MRI: Acquisition of a High-Throughput Fluorescence Slide-Scanner Microscope for the University of Oregon Histology Core Facility

MRI：为俄勒冈大学组织学核心设施购置高通量荧光幻灯片扫描显微镜

• 批准号: 2019156
• 负责人: Cristopher Niell
• 金额: $ 29.98万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019156&HistoricalAwards=false
• 关键词: MRI; Acquisition; Throughput; Fluorescence; Slide

An award is made to the University of Oregon Eugene to support the purchase of a high-throughput fluorescent slide-scanner imaging system. High throughput analysis of biological specimens is increasingly essential for advancing many fields of the life sciences. A key bottleneck, particularly in the study of histological specimens, is the need for manual steps that limit the speed of data acquisition. The new slide scanning microscope will automate many of the manual steps to remove critical bottlenecks and enable high throughput analysis of biological specimens by researchers at the University of Oregon and beyond. The new instrument will be housed in the University of Oregon shared Histology and Genetic Modification Research Core Facility. As part of a core facility, this instrument will enhance training of undergraduate and graduate students and postdoctoral scholars with cutting edge microscopy approaches, advanced image analysis, “big data”, and other key quantitative skills. The system will further accelerate student research during summer REU programs and will also be used in outreach programs and shared with neighboring institutions.The new system will be used for a wide range of biological studies, including gene expression, anatomical reconstructions, and tissue analysis, as well as contributing to undergraduate and graduate coursework and training. Two features of this automated instrument will transform the ability of UO researchers to perform these types of studies. First, it automatically images large area specimens, stitching together individual high magnification fields of view into a single high-resolution image. Second, it can automatically process a large number of specimens, with an autoloader capable of processing up to 200 slides in succession. These capabilities will allow researchers to acquire complete datasets that are simply not tractable with manual imaging, thereby opening up new types of biological questions. The instrument has both brightfield and fluorescent capabilities, enabling a wide range of applications including in situ hybridization analysis of gene expression, reconstruction of large-scale neural organization, and quantification of anatomical and cellular structures. Results from these studies will be disseminated in peer-reviewed journals, through presentation at scientific meetings, and as part of public outreach efforts.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

向俄勒冈大学的尤金大学颁发了奖励，以支持购买高通量荧光幻灯片扫描式成像系统。生物标本的高吞吐量分析对于推进生命科学的许多领域而言越来越重要。一个关键的瓶颈，特别是在组织学标本的研究中，是需要限制数据获取速度的手动步骤。新的幻灯片扫描显微镜将自动化许多手动步骤，以消除关键瓶颈，并能够对俄勒冈大学及其他地区的研究人员对生物标本进行高吞吐量分析。新工具将安置在俄勒冈大学共享的组织学和遗传修饰研究核心设施中。作为核心设施的一部分，该工具将增强对本科生和研究生以及具有尖端显微镜方法的博士后学者的培训，高级图像分析，“大数据”和其他关键的定量技能。该系统将在夏季REU计划中进一步加速学生的研究，还将用于外展计划并与邻近机构共享。新系统将用于广泛的生物学研究，包括基因表达，解剖学重建和组织分析，以及为本科和研究生和培训提供了贡献。这种自动化仪器的两个特征将改变UO研究人员进行此类研究的能力。首先，它会自动对大面积标本进行图像，将单个高放大视野缝合到单个高分辨率图像中。其次，它可以自动处理大量标本，其自动加载器能够连续处理多达200个幻灯片。这些功能将使研究人员能够获取完整的数据集，而这些数据集根本无法通过手动成像进行处理，从而打开了新型的生物学问题。该仪器具有Brightfield和荧光功能，可以实现广泛的应用，包括对基因表达的原位杂交分析，大规模神经组织的重建以及解剖学和细胞结构的定量。这些研究的结果将在经过同行评审的期刊中，通过科学会议的介绍以及作为公共宣传工作的一部分来传播。该奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和更广泛的影响来通过评估来获得的支持。