MRI: Acquisition of a Liquid Chromatograph, Quadrupole, Time-of-Flight Mass Spectrometer to Enhance Undergraduate Research and Education in the Chemical and Biochemical Sciences

MRI：购买液相色谱仪、四极杆、飞行时间质谱仪，以加强化学和生物化学科学方面的本科研究和教育

• 批准号: 2017788
• 负责人: Paul Edmiston
• 金额: $ 23.22万
• 依托单位: College of Wooster
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2017788&HistoricalAwards=false
• 关键词: MRI; Acquisition; Liquid; Chromatograph; Quadrupole

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. The College of Wooster is acquiring a liquid chromatograph, quadrupole, time-of-flight mass spectrometer (LC-Q-TOF-MS) to support Professor Paul Edmiston and colleague, Jennifer Faust. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized and the ions' masses are measured. This highly sensitive technique allows the structure of molecules in complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In the time-of-flight (TOF) method of mass spectrometry, the mass-to-charge ratio of an ion is determined by the way in which ions are accelerated by an electric field of known strength. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. It also provides training opportunities to many undergraduate students at this institution and serves as a resource for K-12 hands-on research engagement as well as course-embedded research projects designed to broaden diversity as guided by the STEM Success Initiative at the college. The instrument is also utilized by researchers at nearby institutions such a St Cloud State University, Oberlin College, Kenyon College, Ashland University, and Denison UniversityThe award of the mass spectrometer is aimed at enhancing research and education at all levels. The instrument acquisition impacts identification of pharmaceutical transformation products in human-impacted surface waters. The LC-Q-TOF-MS is used for investigations of anthropogenic compounds in Hawaiian estuaries by a researcher from St Cloud State University. In addition, the mass spectrometer provides information for studying the fate and transport of perfluoroalkyl substances (PFAS) in the environment and exploring multiphase reactions of organic components in rainwater. The mass spectrometer is also used to analyze protein cross-linkers to identify redox partners of protein disulfide isomerases and for studying the factors influencing differential allocation of seminal fluid proteins in the fruit fly, D. melanogaster. Finally, the instrument is utilized to study the metabolic role of arginine kinases in bacteria and the use of unnatural amino acids to generate chromophores for fluorescence imaging.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具和化学研究工具计划的支持。 Wooster学院正在购买液相色谱仪，星形杆，飞行时间质谱仪（LC-Q-TOF-MS），以支持Paul Edmiston教授和同事Jennifer Faust。通常，质谱法（MS）是用于识别和表征复杂样品中少量化学物种的关键分析方法之一。在典型的实验中，组件流入质谱仪，并测量离子的质量并测量离子的质量。这种高度敏感的技术允许研究复杂混合物中分子的结构。具有液相色谱仪的仪器可以在到达质谱仪之前将其分离化合物的混合物。在飞行时间（TOF）的质谱法（TOF）方法中，离子的质量与电荷比由离子通过已知强度的电场加速的方式确定。此次收购加强了大学和地区地区的研究基础设施。该仪器通过使用这种现代分析技术参与研究和研究培训的各种学生来扩大参与。它还为该机构的许多本科生提供了培训机会，并为K-12动手研究参与度以及课程所在的研究项目提供了资源，旨在扩大学院的STEM成功计划的指导。该工具还被附近机构的研究人员使用，例如St Cloud State University，Oberlin College，Kenyon College，Ashland University和Denison University，质谱仪奖旨在增强各级研究和教育。仪器采集会影响人类影响地表水中药物转化产物的鉴定。 LC-Q-TOF-MS用于研究St Cloud State University的研究人员对夏威夷河口的人为化合物的研究。此外，质谱仪还提供了用于研究环境中全氟烷基物质（PFA）的命运和运输的信息，并探索有机成分在雨水中的多相反应。质谱仪还用于分析蛋白质交联，以鉴定蛋白质二硫异构酶的氧化还原伴侣，并研究影响果蝇中精液蛋白差异分配的因素。最后，该仪器用于研究精氨酸激酶在细菌中的代谢作用，并使用不自然的氨基酸来生成用于荧光成像的发色团。该奖项反映了NSF的法定任务，并被认为是通过使用该基金会的知识分子和更广泛影响的评估来评估CRITERIA CRITERIA通过评估的支持。

项目成果

Suspect screening for pesticides in rain and snow using liquid chromatography high-resolution mass spectrometry

利用液相色谱高分辨质谱法筛查雨雪中农药疑似物

• DOI: 10.1016/j.atmosenv.2022.119389
• 发表时间: 2022
• 期刊: Atmospheric Environment
• 影响因子: 5
• 作者: Gray, Rebekah;Painter, Eve;Sprankle, Jameson W.;Crawford, Alex D.;Morrison, Jillian J.;Frazier, Marian;Faust, Jennifer A.
• 通讯作者: Faust, Jennifer A.

Contaminants of Emerging Concern in the Lower Volta River, Ghana, West Africa: The Agriculture, Aquaculture, and Urban Development Nexus

• DOI: 10.1002/etc.5279
• 发表时间: 2022-01-20
• 期刊: ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
• 影响因子: 4.1
• 作者: Chester, Anndee Huff;Gordon, Christopher;Schoenfuss, Heiko L.
• 通讯作者: Schoenfuss, Heiko L.

Non-targeted identification and semi-quantitation of emerging per-and polyfluoroalkyl substances (PFAS) in US rainwater.

美国雨水中新出现的全氟烷基物质和多氟烷基物质 (PFAS) 的非目标鉴定和半定量。

• 发表时间: 2022
• 期刊: Environmental science Processes impacts
• 作者: Kim, Yubin;Pike, Kyndal A.;Gray, Rebekah;Sprankle, Jameson W.;Faust, Jennifer A.;and Edmiston;Paul L.
• 通讯作者: Paul L.