Collaborative Research: Microbial hydrogen oxidation at high pressure: Role of hydrogenases and interspecies hydrogen transfer

合作研究：高压微生物氢氧化：氢化酶和种间氢转移的作用

• 批准号: 1951673
• 负责人: Dionysios Foustoukos
• 金额: $ 22.86万
• 依托单位: Carnegie Institution of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1951673&HistoricalAwards=false
• 关键词: Collaborative; Research; Microbial; hydrogen; oxidation

A large fraction of Earth’s microbial biosphere lives in the deep ocean and below the oceanic crust, under conditions of high hydrostatic pressures. However, very little is known about the contribution of these high-pressure adapted microbial communities to the Earth’s microbiome, their role in biogeochemical cycles, and their relevance for the evolution of early life on Earth. This project will investigate the adaptation mechanisms of high-pressure and high-temperature bacteria that use hydrogen as an energy source to different pressures and hydrogen concentration regimes. An innovative instrument for bacterial cultures at high pressure and temperature will be used to simulate the deep-sea physical (temperature, pressure) and chemical (hydrogen concentration) conditions. This experimental approach provides a unique opportunity to study microbial activity and functions by adjusting pressure conditions dynamically. Gene expression under different pressures and temperatures will also be monitored. Finally, since hydrogen oxidation is considered an ancient metabolic pathway, understanding hydrogen catabolism in these organisms may help to reconstruct the evolutionary history of early metabolism. The researchers will develop lessons and activities to translate the science for middle and high school aged learners. Lectures and lab demonstrations/tours will be delivered at George Mason University and Rutgers University to undergraduate/graduate students as part of graduate-level seminar series.Culture-based studies of the physiological and metabolic adaptations of high-pressure adapted bacteria are critical to advance understanding of microbial activity and bioenergetic adaptation strategies in deep-sea ecosystems. The main objective of this study is to explore the physiology and gene expression in high-pressure and high-temperature adapted bacteria (thermopiezophiles) that have been isolated from deep-sea hydrothermal vents. One of these bacteria, Nautilia strain PV-1, thrives at elevated pressure and at a temperature of 55°C, and can live off hydrogen gas and carbon dioxide. Experiments aimed at measuring gene and protein expression will be integrated with measurements of stable hydrogen isotope compositions to understand the combined effects of pressure and hydrogen concentration of the growth of thermopiezophiles. More specifically, this project will investigate the expression of the different hydrogenases of strain PV-1 in response to pressures up to 400 atmospheres, and to limiting and non-limiting concentration of hydrogen. Further, this project will investigate how elevated pressures affect the membrane structure of strain PV-1, which is important to maintain cellular integrity and to facilitate membrane trafficking. Finally, this project will investigate the transfer of hydrogen gas from a hydrogen-producing bacterium, Marinitoga piezophila, and the hydrogen consuming Nautilia strain PV-1.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

地球微生物生物圈的很大一部分生活在高静水压力条件下的深海和洋壳之下，然而，人们对这些适应高压的微生物群落对地球微生物组的贡献及其作用知之甚少。生物地球化学循环及其与地球早期生命进化的相关性该项目将研究以氢为能源的高压和高温细菌对不同压力和氢浓度的适应机制。高压和高温细菌培养的创新仪器将用于模拟深海物理（温度、压力）和化学（氢浓度）条件，这种实验方法为研究微生物活动和功能提供了独特的机会。最后，由于氢氧化被认为是一种古老的代谢途径，了解这些生物体中的氢分解代谢可能有助于重建早期代谢的进化历史。课程和活动作为研究生水平研讨会系列的一部分，将在乔治梅森大学和罗格斯大学向本科生/研究生提供讲座和实验室演示/参观，以将科学转化为中学生和高中生。高压适应细菌的代谢适应对于增进对深海生态系统微生物活动和生物能适应策略的理解至关重要。这项研究的主要目的是探索高压和高压下的生理学和基因表达。从深海热液喷口分离出的适应高温的细菌（嗜热压电菌）是其中一种细菌，Nautilia 菌株 PV-1，在高压和 55°C 的温度下繁殖，可以依靠氢气和水生存。旨在测量基因和蛋白质表达的实验将与稳定氢同位素组成的测量相结合，以了解压力和氢浓度对嗜热压电体生长的综合影响。将研究菌株 PV-1 的不同氢化酶响应高达 400 个大气压以及限制和非限制氢浓度的表达。此外，该项目将研究升高的压力如何影响菌株 PV-的膜结构。 1，这对于维持细胞完整性和促进膜运输非常重要。最后，该项目将研究产氢细菌 Marinitoga piezophila 和耗氢 Nautilia 菌株的氢气转移。 PV-1。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Physiological and metabolic responses of chemolithoautotrophic NO3− reducers to high hydrostatic pressure

化能自养型NO3还原剂对高静水压的生理和代谢反应

• DOI: 10.1111/gbi.12522
• 发表时间: 2022
• 期刊: Geobiology
• 影响因子: 3.7
• 作者: Pérez‐Rodríguez, Ileana;Sievert, Stefan M.;Fogel, Marilyn L.;Foustoukos, Dionysis I.
• 通讯作者: Foustoukos, Dionysis I.

Diagnostic biosignature transformation under simulated martian radiation in organic-rich sedimentary rocks

模拟火星辐射下富含有机物沉积岩的诊断生物特征转化

• DOI: 10.3389/fspas.2022.919828
• 发表时间: 2022
• 期刊: Frontiers in Astronomy and Space Sciences
• 影响因子: 3
• 作者: Roussel, A.;McAdam, A. C.;Graham, H. V.;Pavlov, A. A.;Achilles, C. N.;Knudson, C. A.;Steele, A.;Foustoukos, D. I.;Johnson, S. S.
• 通讯作者: Johnson, S. S.