Collaborative Research: NCS-FR: Shedding light on brain circuits mediating navigation of the odor plume in a natural environment

合作研究：NCS-FR：揭示自然环境中介导气味羽流导航的大脑回路

• 批准号: 1926668
• 负责人: Juliet Gopinath
• 金额: $ 109.35万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1926668&HistoricalAwards=false
• 关键词: Collaborative; Research; NCS; FR; Shedding

Animals have a keen ability to find odor sources such as food, partners, pups and predators through the sense of smell in a manner that cannot be replicated by machines. How brains mediate navigation of the environment (the odor plume) through smell is an important unsolved problem. Indeed there are many instances where using machines to navigate an odor landscape is an unmet need to society. For example, even though their training requires lengthy one on one interaction with a trainer, dogs are still used to find explosives in airports and in the battlefield. Our multidisciplinary Odor Plume Neurophotonics (OPeN) team will tackle understanding the brain circuits mediating odor plume navigation. This is a daunting task because it involves characterizing how odors diffuse in the air (environmental engineering), developing advanced miniature microscopes to record from the brain of freely moving animals (electrical and mechanical engineering), recording from brain regions processing odor plume information in real time (systems neuroscience and integrative neurophotonics), and developing mathematical procedures to quantify how the information necessary for successful odor plume navigation is represented in the brain (applied mathematics). Our team will engineer a novel miniature microscope to record activity as mice navigate the odor plume and will assess how the activity of these neurons result in successful odor plume navigation. Furthermore, the team members of OPeN are thoroughly committed to foster advancement of women, underrepresented minorities, veterans and disabled individuals in science and participate in various programs to promote science diversity. Additionally, the team members have a track record of disseminating their work and have an established partnership with a local small business, Intelligent Imaging Innovation, Inc. with world headquarters located in Denver. We will continue our commercial dissemination efforts of the technology developed in this project. Finally, we will endeavor to communicate science to the broader audience through venues such as Scientific American, Public Broadcasting Service and outreach through the Denver Museum of Nature and Science.Members of our OPeN interdisciplinary team developed a novel two photon fiber-coupled microscope for 3D imaging of brain activity in the freely moving mouse and generated and quantified realistic odor environments in the laboratory to explore algorithms used for odor-guided navigation. In this project we leverage the extensive expertise and achievements of the team to crack the circuit basis for odor plume navigation. We will develop a low-weight, miniature 3-photon fiber coupled microscope (3P-FCM) to record neuronal activity simultaneously in one brain area in two planes of view. In addition, OPeN will develop a portable photoionization (PID) sensor to detect the odorant concentration at the nostril as the animal navigates the odor plume. Members of the OPeN team will record neural activity in the hippocampus and cerebellum of animals navigating the odor plume and will develop a Bayesian analysis method to decode odor plume navigation from neural activity. This multidisciplinary approach will result in understanding of the brain mechanisms of odor plume navigation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

动物具有敏锐的能力，能够通过嗅觉寻找食物、伴侣、幼崽和捕食者等气味源，而机器无法复制这种能力。大脑如何通过气味调节环境（气味羽流）的导航是一个尚未解决的重要问题。事实上，在很多情况下，使用机器来驾驭气味景观是社会未满足的需求。例如，尽管它们的训练需要与训练员进行长时间的一对一互动，但狗仍然被用来在机场和战场上寻找爆炸物。我们的多学科气味羽流神经光子学 (OPeN) 团队将致力于了解介导气味羽流导航的大脑回路。这是一项艰巨的任务，因为它涉及表征气味如何在空气中扩散（环境工程），开发先进的微型显微镜来记录自由移动动物的大脑（电气和机械工程），记录大脑区域真实处理气味羽流信息时间（系统神经科学和综合神经光子学），并开发数学程序来量化成功气味羽流导航所需的信息如何在大脑中表示（应用数学）。我们的团队将设计一种新型微型显微镜来记录小鼠在气味羽流中导航时的活动，并将评估这些神经元的活动如何导致成功的气味羽流导航。此外，OPeN 的团队成员完全致力于促进女性、代表性不足的少数族裔、退伍军人和残疾人在科学领域的进步，并参与各种促进科学多样性的项目。此外，团队成员在传播他们的工作方面有着良好的记录，并与当地一家小型企业——Intelligent Imaging Innovation, Inc.（全球总部位于丹佛）建立了合作伙伴关系。我们将继续对该项目中开发的技术进行商业传播。最后，我们将努力通过《科学美国人》、公共广播服务等场所以及丹佛自然与科学博物馆的外展活动向更广泛的受众传播科学。我们 OPeN 跨学科团队的成员开发了一种新型双光子光纤耦合 3D 显微镜对自由移动的小鼠的大脑活动进行成像，并在实验室中生成和量化真实的气味环境，以探索用于气味引导导航的算法。在这个项目中，我们利用团队广泛的专业知识和成就来破解气味羽流导航的电路基础。我们将开发一种轻量、微型三光子光纤耦合显微镜（3P-FCM），以在两个视图平面中同时记录一个大脑区域的神经元活动。此外，OPeN 将开发一种便携式光电离 (PID) 传感器，用于在动物穿过气味羽流时检测鼻孔的气味浓度。 OPeN 团队的成员将记录动物海马体和小脑中气味羽流导航的神经活动，并将开发一种贝叶斯分析方法，从神经活动中解码气味羽流导航。这种多学科方法将有助于理解气味羽流导航的大脑机制。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Suppression of External Vibrations using an Electrowetting Lens

使用电润湿透镜抑制外部振动

• DOI: 10.1109/ipc53466.2022.9975495
• 发表时间: 2022-11
• 期刊: 2022 IEEE Photonics Conference (IPC
• 作者: Miscles, Eduardo J.;Lim, Wei Yang;Supekar, Omkar D.;Zohrabi, Mo;Gopinath, Juliet T.;Bright, Victor M.
• 通讯作者: Bright, Victor M.

GRINtrode: a neural implant for simultaneous two-photon imaging and extracellular electrophysiology in freely moving animals

GRINtrode：一种神经植入物，用于在自由活动的动物中同时进行双光子成像和细胞外电生理学

• DOI: 10.1117/1.nph.9.4.045009
• 发表时间: 2022-10
• 期刊: Neurophotonics
• 影响因子: 5.3
• 作者: McCullough, Connor M.;Ramirez;Hall, Michael;Futia, Gregory L.;Moran, Andrew K.;Gibson, Emily A.;Restrepo, Diego
• 通讯作者: Restrepo, Diego

A Dense Conformal Electrode Array for High Spatial Resolution Stimulation of Electrosensory Systems

用于电传感系统高空间分辨率刺激的密集适形电极阵列

• DOI: 10.1002/admt.202200354
• 发表时间: 2022-08
• 期刊: Advanced Materials Technologies
• 影响因子: 6.8
• 作者: Kumar, Vikrant;Yu, Caroline;McGinn, Christine K.;Perks, Krista E.;Thompson, Sarah M.;Sawtell, Nathaniel B.;Kymissis, Ioannis
• 通讯作者: Kymissis, Ioannis

Molecular layer interneurons in the cerebellum encode for valence in associative learning

小脑中的分子层中间神经元编码联想学习中的效价

• DOI: 10.1038/s41467-020-18034-2
• 发表时间: 2020-08
• 期刊: Nature Communications
• 影响因子: 16.6
• 作者: Ma, Ming;Futia, Gregory L.;de Souza, Fabio M. Simoes;Ozbay, Baris N.;Llano, Isabel;Gibson, Emily A.;Restrepo, Diego
• 通讯作者: Restrepo, Diego

Distortion of passive scalar structure during suction-based plume sampling

基于吸力的羽流采样过程中被动标量结构的畸变

• DOI: 10.1016/j.snb.2022.132018
• 发表时间: 2022-05
• 期刊: Sensors and Actuators B: Chemical
• 作者: True, Aaron C.;Crimaldi, John P.
• 通讯作者: Crimaldi, John P.