CSBR: Living Stocks - Enhancement of the Caenorhabditis Natural Diversity Resource

CSBR：活畜 - 增强秀丽隐杆线虫自然多样性资源

• 批准号: 1930382
• 负责人: Erik Andersen
• 金额: $ 70.81万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1930382&HistoricalAwards=false
• 关键词: CSBR; Living; Stocks; Enhancement; Caenorhabditis; CSBR; Living; Stocks; Enhancement; Caenorhabditis

Studies of conserved biological processes require model organisms which share attributes with humans. Most model organisms are easily grown in the laboratory and enable experimental manipulations. Over the last forty years, the nematode Caenorhabditis elegans has been instrumental in numerous fundamental advances in genetics, developmental biology, and neurobiology. However, all of these discoveries come from studies of a single strain in the species. This limitation is similar to the argument that we can understand all of human biology by the study of a single person in our species. This project seeks to expand the C. elegans Natural Diversity Resource (CeNDR), which comprises nearly 1000 genetically distinct strains from around the world. This genetic diversity facilitates discoveries about the evolution of physiological and behavioral traits, including responses to chemical and biotic stresses. Because humans share many of the same genes and processes as this nematode species, studies using this organism can teach us a great deal about how the human population differs.CeNDR has three major goals: (1) Collect, organize, and disseminate wild C. elegans strains; (2) Organize and disseminate whole-genome sequence and variant data for wild strains; and (3) Provide a genetics portal to facilitate mappings and interpretations of results. This project will address critical improvements to the methodology and technology of each of those goals. These improvements will enable future discoveries with CeNDR and will enable the planned expansion to include strains, whole-genome data, and mappings for two related nematode species: C. briggsae and C. tropicalis. This expansion to the Caenorhabditis Natural Diversity Resource (CaeNDR) will build unparalleled new capacity for comparative evolution approaches in nematodes and likely other organisms. Most research groups do not have the combination of experimental, computational, and statistical training to easily perform studies of natural variation. This project will make these tools available and usable by research laboratories across diverse model organism communities.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

对保守生物过程的研究需要与人类共享属性的模型生物。 大多数模型生物都很容易在实验室中生长并实现实验操作。 在过去的四十年中，线虫秀丽隐杆线虫在遗传学，发育生物学和神经生物学方面的众多基本进展中发挥了作用。 但是，所有这些发现均来自该物种中的单个菌株的研究。 这种局限性类似于我们可以通过对我们物种中一个人的研究来理解所有人类生物学的论点。 该项目旨在扩大秀丽隐杆线虫自然多样性资源（CENDR），该资源包括来自世界各地的近1000个遗传菌株。 这种遗传多样性促进了关于生理和行为特征进化的发现，包括对化学和生物应激的反应。 由于人类与该线虫物种共享许多相同的基因和过程，因此使用这种生物体的研究可以教给我们有关人类人口如何差异的大量知识。CENDR具有三个主要目标：（1）收集，组织和传播野生的秀丽隐杆线虫菌株； （2）组织和传播野生菌株的全基因组序列和变体数据； （3）提供遗传学门户，以促进结果的映射和解释。 该项目将解决对每个目标的方法和技术的重要改进。 这些改进将使Cendr的未来发现，并使计划的扩展能够包括菌株，全基因组数据和两个相关线虫物种的映射：C. briggsae和C. tropicalis。 这种扩展到Caenorhabditis自然多样性资源（CAENDR）将建立无与伦比的线虫和可能其他生物中比较进化方法的新能力。 大多数研究小组没有实验，计算和统计培训的组合，可以轻松进行自然变异研究。 该项目将使这些工具可用于各种模型有机体社区的研究实验室可用，并且可以使用该基金会的知识分子优点和更广泛的影响审查标准，反映了NSF的法定任务，并被认为值得通过评估。