Research Initiation Award: Metagenomic Approach to Assess Water Quality and Microbial Load Variability of an Urban Watershed

研究启动奖：评估城市流域水质和微生物负荷变异性的宏基因组方法

• 批准号: 1900701
• 负责人: Ethell Vereen
• 金额: $ 30万
• 依托单位: Morehouse College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1900701&HistoricalAwards=false
• 关键词: Research; Initiation; Award; Metagenomic; Approach

Research Initiation Awards provide support for junior and mid-career faculty at Historically Black Colleges and Universities who are building new research programs or redirecting and rebuilding existing programs. It is expected that the award helps to further the faculty member's research capability and effectiveness, improves research and teaching at the home institution, and involves undergraduate students in research experiences. The study will enhance the understanding of the composition, diversity, and richness of bacterial communities by evaluating the microbial community, characterize variability in time and space and compare microbial community between an urban watershed and an unimpacted watershed.The development of microbiome studies based on DNA sequencing has led to more thorough investigations of the effect of environmental conditions on microbial community composition. Despite recent advances in sequencing technologies, identifying the ecological mechanisms that underlie microbial community structure and functional diversity remains a major challenge. This challenge is particularly important for highly dynamic ecosystems such as flowing-waters of urban systems. This project will provide new information on how microbial communities persist and change within an urban watershed using metagenomic techniques. It is expected that temporally, the microbial community in the fall and winter months will be distinct from the microbial community in the spring and summer months, while spatially, the microbial community immediately downstream from anthropogenic inputs will be distinct from natural systems. The findings from this study will improve our understanding of the way urbanization shapes stream microbial communities and provide insight of how microbial communities persist and change within an urban watershed.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

研究启动奖为历史悠久的黑人学院和大学的初级和中级教师提供了支持，他们正在构建新的研究计划，或重新指导和重建现有计划。预计该奖项有助于进一步促进教职员工的研究能力和有效性，改善家庭机构的研究和教学，并让本科生参与研究经验。这项研究将通过评估微生物群落来增强对细菌群落的组成，多样性和丰富性的理解，表征时间和空间的变化，并比较城市流域和未冲击的流域之间的微生物群落。基于DNA测序的微生物组研究的发展使DNA测序的发展具有更彻底的彻底研究，从而更加彻底地研究了对环境社区的构成的效应。尽管测序技术最近取得了进步，但确定了基于微生物群落结构和功能多样性的生态机制仍然是一个主要挑战。对于高度动态的生态系统，例如城市系统的流动潮流，这一挑战尤为重要。该项目将提供有关微生物群落如何使用元基因组技术在城市流域内持续和变化的新信息。可以预期，秋季和冬季的微生物群落在春季和夏季的微生物社区将与微生物群落不同，而在空间上，微生物群落立即下游人类学输入与自然系统不同。这项研究的发现将提高我们对城市化塑造微生物群落的方式的理解，并洞悉微生物社区如何在城市流域内保持和变化。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查审查标准来通过评估来获得支持的。