Collaborative Research: Cranial Base Development in Primates

合作研究：灵长类动物的颅底发育

• 批准号: 1830919
• 负责人: Tim Smith
• 金额: $ 10.95万
• 依托单位: Slippery Rock University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1830919&HistoricalAwards=false
• 关键词: Collaborative; Research; Cranial; Base; Development

This project will address questions about primate and human biology using cutting-edge visualization techniques to map the cellular processes involved in growth and development of cranial cartilage and bone across a wide range of primates. Findings from this project will advance knowledge about the ways in which cranial growth determines overall skull shape, which is important for understanding primate adaptations and life history through time. The project will support research infrastructure and training of young scientists, and may inform clinical research and treatments for children with cranial malformations.This study relates differences in cranial base structure (including degree of basicranial flexion and facial orientation) to direct evidence of growth processes. Nine primate species are studied using a novel combination of micro-computed tomography and histological procedures to create 3D virtual reconstructions of the developing skull. Growth patterns in cranial base synchondroses are quantified using linear and cross-sectional area measurements of cartilaginous zones. Cranial shape in perinatal and older subadults is described using volumetric, angular, and landmark-based morphometric data. A combination of descriptive and inferential statistics are applied to address four specific aims: 1) describe the structure of the perinatal cranial base in a comparative sample of primates; 2) document evidence of cellular and tissue-level mechanisms contributing to growth; 3) test for a phylogenetic effect in perinatal cranial base structure; and 4) test the association of cranial base growth patterns with cranial base angle and facial orientation in the perinatal period and in the adult. This project is the first to assess early postnatal synchondroseal patency or fusion in a broad range of primates and advance understanding of growth characteristics of bone and cartilage by directly observing indicators of chondrocyte proliferation and hypertrophy, two drivers of interstitial growth.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将使用尖端的可视化技术解决有关灵长类动物和人类生物学的问题，以绘制跨广泛的灵长类动物的颅软骨和骨骼的生长和发展所涉及的细胞过程。 该项目的发现将促进有关颅增长决定整体颅骨形状的方式的知识，这对于了解灵长类动物的适应和生活历史很重要。该项目将支持年轻科学家的研究基础设施和培训，并可能为患有颅骨畸形的儿童的临床研究和治疗提供信息。这项研究将颅底结构的差异（包括基本界屈曲和面部取向程度）指导生长过程的证据。使用微型断层扫描和组织学程序的新型组合研究了9种灵长类动物，以创建发育中的头骨的3D虚拟重建。 使用线性和横截面区域测量软骨区域对颅底同步性的生长模式进行定量。 使用体积，角和里程碑的形态计量数据来描述围产期和较旧亚地区的颅骨形状。 描述性统计和推论统计的结合用于解决四个具体目的：1）描述灵长类动物比较样本中围产期颅底的结构； 2）记录有助于生长的细胞和组织水平机制的证据； 3）测试围产期颅底结构中的系统发育效应； 4）在围产期和成年人中测试颅底生长模式与颅底角度和面部取向的关联。该项目是第一个通过直接观察软骨细胞增殖和肥大的指标来评估广泛的灵长类动物和融合骨骼和软骨的生长特征的早期早期同步的通畅或融合的早期项目，这两种奖励反映了NSF的法定任务和基础，这是两种依据的依据，这反映了众多的依据。 标准。

项目成果

Cranial synchondroses of primates at birth

灵长类动物出生时颅骨软骨病

• DOI: 10.1002/ar.24521
• 发表时间: 2020
• 期刊: The Anatomical Record
• 作者: Smith, Timothy D.;Reynolds, Rebecca L.;Mano, Nanami;Wood, Brody J.;Oladipupo, Lanre;Hughes, Gabriel K.;Corbin, Hayley M.;Taylor, Jane;Ufelle, Alexander;Burrows, Anne M.
• 通讯作者: Burrows, Anne M.

Midline growth of the sphenoid bone in primates: A histological and microcomputed tomography study

灵长类动物蝶骨的中线生长：组织学和显微计算机断层扫描研究

• DOI: 10.1002/ajpa.24653
• 发表时间: 2022
• 期刊: American Journal of Biological Anthropology
• 作者: Wood, Brody;Oladipupo, Lanre;Mano, Nanami;Taylor, Jane;Vinyard, Christopher J.;Cray, James;DeLeon, Valerie B.;Smith, Timothy D.
• 通讯作者: Smith, Timothy D.

Venous networks in the upper airways of bats: A histological and diceCT study

蝙蝠上呼吸道的静脉网络：组织学和 diceCT 研究

• DOI: 10.1002/ar.24762
• 发表时间: 2022
• 期刊: The Anatomical Record
• 作者: Smith, Timothy D.;DeLeon, Valerie B.;Eiting, Thomas P.;Corbin, Hayley M.;Bhatnagar, Kunwar P.;Santana, Sharlene E.
• 通讯作者: Santana, Sharlene E.

“Mucosal maps” of the canine nasal cavity: Micro‐computed tomography and histology

犬鼻腔的“粘膜图”：显微计算机断层扫描和组织学

• DOI: 10.1002/ar.24511
• 发表时间: 2020
• 期刊: The Anatomical Record
• 作者: Smith, Timothy D.;Craven, Brent A.;Engel, Serena M.;Van Valkenburgh, Blaire;DeLeon, Valerie B.
• 通讯作者: DeLeon, Valerie B.

Inward collapse of the nasal cavity: Perinatal consolidation of the midface and cranial base in primates

鼻腔向内塌陷：灵长类动物中面部和颅底的围产期实变

• DOI: 10.1002/ar.24537
• 发表时间: 2020
• 期刊: The Anatomical Record
• 作者: Smith, Timothy D.;Ufelle, Alexander C.;Cray, James J.;Rehorek, Susan B.;DeLeon, Valerie B.
• 通讯作者: DeLeon, Valerie B.