SNM-IS: Scalable Biomineralization of Functional Oxide Nanoparticles and Nanostructures for Environmental and Energy Applications

SNM-IS：用于环境和能源应用的功能性氧化物纳米颗粒和纳米结构的可扩展生物矿化

基本信息

批准号：
1821389
负责人：
Bryan Berger
金额：
$ 149.96万
依托单位：
University of Virginia Main Campus
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-10-09 至 2024-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1821389&HistoricalAwards=false
关键词：
SNM Scalable Biomineralization Functional Oxide

项目摘要

This research project seeks to create a scalable, green, continuous process for biomineralization of nanoparticles directly from aqueous solutions at room temperature and to create structured catalysts for automotive and other industrial applications. Biomineralization is the process by which biological systems produce inorganic minerals, which display nanostructured features that are otherwise difficult to achieve. Nanostructured minerals are essential components in a number of industrially-important processes and products, in which control over the particle size is key to performance. As one example, certain nanostructured minerals, such as ceria, are used in automobile emission control, to remove carbon monoxide and other environmentally harmful exhaust gases. Current industrial methods for nanostructured ceria production often require high temperatures, high pressures and toxic solvents, thus limiting their utility. This research project involves studying methods to overcome these limitations by engineering enzymes as biocatalysts for the large-scale production of nanostructured ceria. The researchers on this project collaborate with researchers at Cerion, an industrial partner, to understand industrial-scale production issues. This project provides a unique, cross-disciplinary educational opportunity for U.S. graduate and undergraduate students to gain training in synthetic biology, nanoparticle manufacturing and catalysis. It also provides opportunities to partner with leading international institutes such as the Cardiff Catalysis Institute which will enable U.S. students to learn state-of-the-art production and characterization methods. This project will lead to a new, environmentally-friendly process to produce high-value materials and demonstrate their enhanced performance in consumer products such as automobiles. The goal of this research is to develop a robust, green and flexible platform for the high-yield enzymatic synthesis of size-controlled ceria and ceria-zirconia nanoparticles directly from aqueous solutions at room temperature, and to integrate these materials into structured catalyst platforms. The approach is to study and develop engineered silicatein, the enzyme responsible for silica mineralization in sea sponges, to control mineralization of both ceria and ceria-zirconia in a size range, less than 2 nm, that enables functional superiority in primary catalytic applications as compared to conventional chemically-synthesized nanoceria. The fundamental technical barriers to scalable, green nanomanufacturing of these materials are overcome by using directed evolution in engineering enzymes with enhanced nanoceria synthesis rates and integrating them into immobilized enzyme biocatalysts for large-scale nanoceria production. The unique advantages of biomineralization enables the synthesis of smaller, more homogeneous nanoparticles and the direct, enzymatic synthesis of nanomaterials on structured support materials for their integration into catalytic nanosystems. Ultimately, the ability to produce nanoceria directly from aqueous solutions and to control particle size will create the next generation of these important classes of new, emergent nanomaterials at a cost and scale compatible with the needs of industry.

该研究项目旨在在室温下直接从水溶液中直接从水溶液中创建可扩展的，绿色的连续过程，并为汽车和其他工业应用创建结构化催化剂。生物矿化是生物系统产生无机矿物的过程，这些过程表现出难以实现的纳米结构特征。纳米结构矿物是许多工业重要过程和产品中的重要组成部分，其中控制粒度是性能的关键。作为一个例子，某些纳米结构化的矿物质（例如陶瓷）用于汽车排放控制中，以去除一氧化碳和其他环境有害的废气。当前用于纳米结构二氧化碳生产的工业方法通常需要高温，高压和有毒溶剂，从而限制了它们的效用。该研究项目涉及研究通过工程酶作为大规模生产纳米结构二氧化碳的生物催化剂来克服这些局限性的方法。该项目的研究人员与工业合作伙伴Cerion的研究人员合作，以了解工业规模的生产问题。该项目为美国研究生和本科生提供了独特的跨学科教育机会，以获得合成生物学，纳米颗粒制造和催化的培训。它还提供了与加的夫催化学院等领先的国际研究所合作的机会，该研究所将使美国的学生能够学习最先进的生产和表征方法。该项目将导致一个新的，环保的过程，以生产高价值材料，并证明其在消费产品（例如汽车）中的性能提高。这项研究的目的是开发一个健壮，绿色和柔性的平台，以直接从室温下从水溶液中直接从水溶液中的尺寸控制的二氧化碳和二氧化二氧化粒细胞膜进行高收益酶合成，并将这些材料整合到结构化的催化剂平台中。该方法是研究和开发工程化的硅酸盐，硅酸盐是负责海绵中二氧化硅矿化的酶，以在小于2 nm的尺寸范围内控制陶瓷和二氧化碳的矿化，与常规的化学化学纳米环相比，在原发性催化施用中具有功能优势。通过在工程酶中使用定向的进化来克服这些材料的可扩展，绿色纳米制造的基本技术障碍，并具有增强的纳米核合成速率，并将它们整合到固定的酶生物催化剂中，以用于大规模的纳米核生产。生物矿化的独特优势使得较小，更均匀的纳米颗粒以及纳米材料在结构化支持材料上的直接酶合成，以将其整合到催化纳米系统中。最终，直接从水溶液中生产纳米果的能力并控制粒径将创造下一代这些重要类别的新型新兴纳米材料，其成本和规模与行业需求兼容。