Collaborative Research: Investigations into microbially mediated ecological diversification in sponges

合作研究：微生物介导的海绵生态多样化研究

基本信息

批准号：
1756249
负责人：
Robert Thacker
金额：
$ 9.91万
依托单位：
SUNY at Stony Brook
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-01 至 2022-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1756249&HistoricalAwards=false
关键词：
Collaborative Research Investigations into microbially

项目摘要

Coral reefs represent a paradox because, despite their immense productivity and biodiversity, they are found in nutrient-poor habitats that are equivalent to "marine deserts." High biodiversity is often associated with a division of resources that allows many types of organisms to coexist with minimal competition. Indeed, unlike many other organisms on coral reefs, sponges are adapted to efficiently remove bacteria, phytoplankton, and dissolved organic matter from seawater by filter-feeding. Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body. This research will combine manipulative field experiments with a novel combination of modern analytical tools to investigate both filter-feeding by sponge hosts and the metabolic pathways of their microbes. This work will advance our understanding of the ecological and evolutionary forces that have helped shape the species present on Caribbean coral reefs. Additionally, this project will support three early-career investigators and provide training opportunities for graduate and undergraduate students at Nova Southeastern University, Appalachian State University, Stony Brook University, and Smithsonian Marine Station. The investigators will also develop innovative outreach programs that expand existing platforms at their institutions to increase public engagement and scientific literacy. Marine sponges have been widely successful in their expansion across ecological niches in the Caribbean, with biomass often exceeding that of reef-building corals and high species diversity. However, whether this success is linked to efficient heterotrophic filter-feeding on organic carbon in the water column or to their evolutionary investment in microbial symbionts is yet to be fully elucidated. Microbial symbionts expand the metabolic capabilities of host sponges, supplementing heterotrophic feeding with inorganic carbon and nitrogen, mediating the assimilation of dissolved organic matter, and facilitating recycling of host-derived nitrogen. Despite these benefits, microbial symbiont communities are widely divergent across coexisting sponge species and there is substantial variation in host reliance on symbiont-derived carbon and nitrogen among host sponges; therefore, these associations likely mediate the ecological diversification of coexisting sponge species. The goal of this project is to test this transformative hypothesis by adopting an integrative approach to assess the individual components of holobiont metabolism (i.e., microbial symbionts and sponge host) in ten of the most common sponge species in the Caribbean. The investigators will isolate autotrophic and heterotrophic metabolic pathways and explore potential links between microbial symbiont community composition and the assimilation of particulate and dissolved organic matter (POM and DOM) from seawater. This project will elucidate whether Caribbean sponge species are on similar or divergent evolutionary trajectories, and will provide information that is critical for our understanding of how conditions in the Caribbean basin have shaped the evolution of benthic organisms.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

珊瑚礁代表着一个悖论，因为尽管它们具有巨大的生产力和生物多样性，但它们却生活在相当于“海洋沙漠”的营养贫乏的栖息地。高生物多样性通常与资源划分相关，资源划分允许多种生物体以最小的竞争共存。事实上，与珊瑚礁上的许多其他生物不同，海绵能够通过滤食有效地去除海水中的细菌、浮游植物和溶解的有机物。海绵是全世界和加勒比地区珊瑚礁的主要组成部分，其生物量超过了造礁珊瑚。近四分之一个世纪以来，加勒比地区海绵的成功与其滤食能力有关。然而，最近的研究表明，加勒比珊瑚礁上共存的海绵拥有独特的细菌群落，这些细菌可能使海绵能够获得其他生物无法获得的多个营养池。在这个项目中，研究人员将检验这样一个假设：加勒比地区的生态优势海绵物种具有独特的代谢策略，这些策略是由它们与生活在海绵体内的微生物的关联介导的。这项研究将结合现场操作实验和现代分析工具的新颖组合，以研究海绵宿主的滤食及其微生物的代谢途径。这项工作将增进我们对生态和进化力量的理解，这些力量帮助塑造了加勒比珊瑚礁上的物种。此外，该项目还将支持三名早期职业研究人员，并为诺瓦东南大学、阿巴拉契亚州立大学、石溪大学和史密森尼海洋站的研究生和本科生提供培训机会。研究人员还将制定创新的外展计划，扩大其机构的现有平台，以提高公众参与度和科学素养。海洋海绵在加勒比地区生态位的扩张方面取得了广泛成功，其生物量往往超过造礁珊瑚，并且物种多样性很高。然而，这一成功是否与水体中有机碳的高效异养滤食有关，或者与微生物共生体的进化投资有关，尚待充分阐明。微生物共生体扩大了宿主海绵的代谢能力，补充了无机碳和氮的异养摄食，介导溶解有机物的同化，并促进宿主来源的氮的循环利用。尽管有这些好处，微生物共生群落在共存的海绵物种之间存在很大差异，并且宿主海绵对共生体衍生的碳和氮的依赖存在很大差异；因此，这些关联可能调节共存海绵物种的生态多样化。该项目的目标是通过采用综合方法评估加勒比地区十种最常见海绵物种的全生物代谢的各个组成部分（即微生物共生体和海绵宿主）来检验这一变革性假设。研究人员将分离自养和异养代谢途径，并探索微生物共生群落组成与海水中颗粒和溶解有机物（POM 和 DOM）同化之间的潜在联系。该项目将阐明加勒比海绵物种是否处于相似或不同的进化轨迹，并将提供对于我们了解加勒比海盆地的条件如何影响底栖生物进化至关重要的信息。该奖项反映了 NSF 的法定使命，并已被通过使用基金会的智力优点和更广泛的影响审查标准进行评估，认为值得支持。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Host population genetics and biogeography structure the microbiome of the sponge Cliona delitrix

宿主群体遗传学和生物地理学构建了海绵 Cliona delitrix 的微生物组

DOI：
10.1002/ece3.6033
发表时间：
2020-01-24
期刊：
Ecology and Evolution
影响因子：
2.6
作者：
Cole G Easson;A. Chaves‐Fonnegra;R. Thacker;J. Lopez
通讯作者：
J. Lopez

New shallow water species of Caribbean Ircinia Nardo, 1833 (Porifera: Irciniidae)

加勒比海 Ircinia Nardo 浅水新种，1833 年（多孔菌门：Irciniidae）

DOI：
10.11646/zootaxa.5072.4.1
发表时间：
2021-12
期刊：
Zootaxa
影响因子：
0.9
作者：
KELLY, JOSEPH B.;THACKER, ROBERT W.
通讯作者：
THACKER, ROBERT W.

Microbial symbionts and ecological divergence of Caribbean sponges: A new perspective on an ancient association

加勒比海绵的微生物共生体和生态分歧：古老关联的新视角

DOI：
10.1038/s41396-020-0625-3
发表时间：
2020-06
期刊：
The ISME Journal
影响因子：
0
作者：
Freeman, Christopher J.;Easson, Cole G.;Matterson, Kenan O.;Thacker, Robert W.;Baker, David M.;Paul, Valerie J.
通讯作者：
Paul, Valerie J.

Novel trends of genome evolution in highly complex tropical sponge microbiomes

高度复杂的热带海绵微生物组中基因组进化的新趋势