Collaborative Research: RUI SG: Evolutionary relationships and systematics of riverweeds (Podostemaceae)

合作研究：RUI SG：河草（Podostemaceae）的进化关系和系统学

基本信息

批准号：
1754329
负责人：
Patrick Calie
金额：
$ 13.87万
依托单位：
Eastern Kentucky University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-06-01 至 2022-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1754329&HistoricalAwards=false
关键词：
Collaborative Research RUI SG Evolutionary

项目摘要

The riverweed family (Podostemaceae) is the largest entirely aquatic family of flowering plants and provides important habitats and food sources for many aquatic animals. Many of the estimated 300 riverweed species are narrowly distributed and incompletely known, and genealogical relationships within the family are uncertain. This project will produce the first family-wide estimate of genealogical relationships among riverweeds using DNA sequence data, and a better understanding of relationships, morphological variation, and will clarify the number of different species that exist. This project will provide broad training opportunities for undergraduate students in both field and laboratory research, including molecular biology, plant structural analyses, database development and management, and taxonomomy at two Primarily Undergraduate Institutions located in Connecticut and Kentucky. DNA sequence data generated by the project will be disseminated via publicly accessible databases, and newly collected plant specimens will be deposited within natural history museums.This project will produce a molecular systematic and phylogenetic analysis of the flowering plant family Podostemaceae, and a taxonomic monograph of the genus Apiniagia (Podostemaceae). Podostemaceae species exhibit high phenotypic plasticity and have highly modified growth forms, both of which contribute to taxonomic confusion. This project will assemble a well-sampled and robust phylogeny of the family, with a focus on neotropical taxa, using morphological data and molecular data obtained via high throughput DNA sequencing. Molecular data will be collected from whole chloroplast genomes and select mitochondrial and nuclear loci. This phylogeny will be used to circumscribe all neotropical genera of Podostemaceae, understand convergent patterns of morphological evolution, and test the hypothesis that the distribution of the neotropical Podostemoideae clade is largely explained by major river capture events during the Cenozoic. Using the results of the phylogenetic analyses in combination with morphological investigations, a taxonomic monograph for the genus Apiniagia will be produced. This genus is the largest, most morphologically complex, and taxonomically difficult genus of neotropical Podostemaceae. The monograph will include nomenclatural updates, morphological descriptions, and taxonomic keys, in addition to newly developed online resources to broadly disseminate the results. This project will also produce a new, free tool for the systematics community, a Darwin Core Archive export option for the Symbiota platform specific to monographic data sets.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

河草科（Podostemaceae）是最大的水生开花植物科，为许多水生动物提供重要的栖息地和食物来源。估计有 300 种河草物种，其中许多物种分布范围狭窄且不完全已知，并且该科内的谱系关系尚不确定。该项目将利用 DNA 序列数据对河草之间的谱系关系进行首次全科估计，更好地了解关系、形态变异，并将澄清现有不同物种的数量。该项目将为本科生提供广泛的实地和实验室研究培训机会，包括分子生物学、植物结构分析、数据库开发和管理以及位于康涅狄格州和肯塔基州的两个主要本科机构的分类学。该项目生成的 DNA 序列数据将通过公开数据库传播，新收集的植物标本将存放在自然历史博物馆内。该项目将对开花植物科 Podostemaceae 进行分子系统和系统发育分析，并编写一本分类学专着。 Apiniagia (Podostemaceae) 属。足茎科物种表现出高表型可塑性，并且具有高度改变的生长形式，这两者都导致了分类学的混乱。该项目将利用通过高通量 DNA 测序获得的形态学数据和分子数据，构建该科的采样良好且稳健的系统发育学，重点关注新热带类群。分子数据将从整个叶绿体基因组中收集，并选择线粒体和核基因座。该系统发育学将用于界定所有新热带Podostemaceae属，了解形态进化的趋同模式，并检验新热带Podostemoideae分支的分布在很大程度上是由新生代主要河流捕获事件解释的假设。将系统发育分析的结果与形态学研究相结合，将制作菠菜属的分类学专着。该属是新热带足尾科中最大、形态最复杂、分类学最困难的属。该专着将包括命名法更新、形态描述和分类键，以及新开发的在线资源以广泛传播结果。该项目还将为系统学界提供一个新的免费工具，即专门针对专题数据集的 Symbiota 平台的 Darwin Core Archive 导出选项。该奖项反映了 NSF 的法定使命，并通过使用基金会的知识产权进行评估，被认为值得支持。优点和更广泛的影响审查标准。