MRI/Acquisition: Upgrading the Microbial Ecology and Biotechnology Laboratory at Universidad del Turabo with a more efficient Genetic Analyzer for sequencing and fragment analysis

MRI/采集：使用更高效的基因分析仪进行测序和片段分析，升级图拉博大学的微生物生态学和生物技术实验室

• 批准号: 1726158
• 负责人: Sharon Cantrell
• 金额: $ 21.31万
• 依托单位: Universidad Del Turabo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726158&HistoricalAwards=false
• 关键词: MRI; Acquisition; Upgrading; Microbial; Ecology

An award is made to Universidad del Turabo to upgrade the existing molecular facilities with the acquisition of a more efficient Genetic Analyzer that will enhance current research activities at Universidad del Turabo (UT) and project partners (University of Puerto Rico-Cayey). The project will continue to support the training of undergraduate and graduate students under different research activities and recently funded initiatives to Universidad del Turabo. The project will use Sanger-Sequencing and Fragment Analysis in different projects that will provide baseline data for other research projects. All projects will foster collaboration among project researchers and institutions. The project will also provide training in molecular techniques and in the operation of the state of the art Genetic Analyzer, to augment the future STEM work force through different sponsored projects led by the PI, Co-PI and major instrument users (NSF LTER, NIH-INBRE, USDA and PRSTRT at UT and HHMI SEA-PHAGES of UPR-Cayey). UPR-Cayey has also developed a Research Experiences for Teachers (RET) Program, supported by HHMI that provides training to pre-service teachers (pedagogy majors) in original research in Microbial Ecology that will make use of the Genetic Analyzer. Peer reviewed publications and scientific presentations at local and national meetings will be produced as the results of the different research projects are generated.The requested Genetic Analyzer (ABI3500) provides faster, easier and better quality control for sequencing and fragment analysis. The UT Microbial Ecology and Biotechnology Laboratory (MEBL) has been essential in the training of undergraduate and graduate students, faculty and teachers in the use of genomic techniques such as Sanger Sequencing and Fragment Analysis to study microbial communities. The project brings together seven (7) UT and University of Puerto Rico-Cayey researchers that represent two primary undergraduate minority serving Hispanic institutions. All research projects have demonstrated the need of the new genetic analyzer ABI 3500 and how the instrument will benefit the training of students at all levels, as well as school teachers. The twelve (12) research projects address the following topics: water quality, aeromycology, tropical rain forest microbial ecology, bioremediation, agro-microbiome, bioprospecting, biochemistry (DNA-conjugated quantum dots), Drosophila population genetics, avian DNA barcoding, and genomics of bacteriophages and bacteria. All these themes are very important for the improvement and understanding of climate change, environmental protection and human and environmental health.

通过收购更有效的遗传分析仪，将授予Turabo大学的奖励，以升级现有的分子设施，该机构将增强Turabo大学（UT）和Project Partners（波多黎各分校）的当前研究活动。该项目将继续支持在不同的研究活动下对本科生和研究生的培训，并向最近向Turabo大学提供了资助。该项目将在不同的项目中使用Sanger-Sequering和Fragment分析，这些分析将为其他研究项目提供基线数据。所有项目都将促进项目研究人员和机构之间的合作。该项目还将提供分子技术的培训以及最先进的遗传分析仪状态的运行，以通过PI，Co-Pi和主要仪器使用者（NSF LTER，NIHIH-INBRE，USDA，USDA和PRSTRT在UT和HHMI Sea-apprt of UPR-Cayey的OUPR-Cayey）领导的不同赞助的项目来增强未来的STEM劳动力。 UPR-Cayey还为HHMI支持的教师（RET）计划开发了一项研究经验，该研究在微生物生态学的原始研究中为职前教师（教育学专业）提供了培训，该研究将利用遗传分析仪。 随着不同的研究项目的结果，将在本地和国家会议上进行同行审查的出版物和科学演示。要求的遗传分析仪（ABI3500）为测序和碎片分析提供了更快，更容易，更轻松，更好的质量控制。 UT微生物生态学和生物技术实验室（MEBL）对于在使用基因组技术（例如Sanger测序和片段分析）研究微生物社区等基因组技术方面对培训本科生和研究生，教职员工和教师至关重要。该项目汇集了七（7）个UT和波多黎各大学的研究人员，这些研究人员代表了两个为西班牙裔机构服务的小学本科生。 所有研究项目都表明了新的遗传分析仪ABI 3500的需求，以及该工具将如何使各级学生和学校教师的培训受益。十二（12）个研究项目介绍了以下主题：水质，航空学，热带雨林微生物生态学，生物修复，农业微生物组，生物培训，生物化学（DNA偶联的量子点），果蝇种群遗传学，AVIAN DNA DNA DNA BACOPTING和GETOMPAGES和GATOMACES和GATOMACES和GATOMACES和GACTERICAGES和GETAMICAGES和GENOMAGES和GENOMAGES和GETAMICAGES和BACTERIA。所有这些主题对于改善和理解气候变化，环境保护以及人类和环境健康非常重要。