MRI: Acquisition of a circular dichroism spectropolarimeter for research and training of undergraduates at Gettysburg College

MRI：购买圆二色性分光偏振仪，用于葛底斯堡学院本科生的研究和培训

• 批准号: 1725534
• 负责人: Shelli Frey
• 金额: $ 11.21万
• 依托单位: Gettysburg College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1725534&HistoricalAwards=false
• 关键词: MRI; Acquisition; circular; dichroism; spectropolarimeter

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation (CRIF) programs. Professor Shelli Frey from Gettysburg College and colleagues Kurt Andresen, Katherine Buettner and Lucas Thompson have acquired a circular dichroism (CD) spectrometer. Many chemical and biological molecules are chiral (having structures that are not superimposable - like our hands). Chiral molecules that are prevalent in living organisms can exist in two forms. These forms can be described as having left-handed or right-handed conformations. A CD spectrometer can determine which form or whether a mixture of both forms is present. The spectrometer can follow changes as the chiral molecule binds to another molecule (ligand), rearranges its structure, or reacts (perhaps with a drug or an antibody). Therefore, CD spectroscopy is suitable for probing protein (or other macromolecular) structures or changes to structures due to environmental conditions. CD spectroscopy has been widely used for three types of measurements: identifying the structure of proteins, studying the geometry changes during folding and unfolding of proteins, and determining the energies involved in binding interactions and protein unfolding. This spectrometer is used by undergraduates. While being trained to use this important tool, the research experience prepares the students for future careers and advanced degrees in medical and scientific fields. The instrument is also used by the NSF funded STEM Scholars program, which fosters a sense of community for students of diverse backgrounds with an interest in science fields. The acquisition of this spectropolarimeter opens new paths of research for students and faculty, and it helps students see more of the interconnected nature of science through the interdisciplinary projects. Students have access to this instrumentation in their courses, ultimately resulting in researchers prepared to enter graduate school or careers in the sciences.This award of a circular dichroism spectrometer is aimed at enhancing research and education at all levels, especially in areas such as:(a) developing titanium and vanadium binding sites in small proteins to determine secondary structure and study DNA cleavage, (b) assembling gold nanorods with electrostatically adsorbed poly-polyl-lysine to understand how molecular structure can translate into control over chiral nanoparticle assemblies, and (c) studying the folding of the nucleosome to give insight into how DNA in the nucleosome can interact with other biological molecules in vivo.

该奖项得到了主要的研究工具（MRI）和化学研究工具（CRIF）计划的支持。葛底斯堡学院和同事Kurt Andresen，Katherine Buettner和Lucas Thompson的Shelli Frey教授已经获得了圆形二科主义（CD）光谱仪。许多化学和生物分子是手性的（具有不可叠加的结构 - 就像我们的手一样）。在生物体中普遍存在的手性分子可以两种形式存在。这些形式可以描述为具有左撇子或右手构象。 CD光谱仪可以确定两种形式的混合物是否存在。由于手性分子与另一个分子（配体）结合，重新排列其结构或反应（也许与药物或抗体）时，光谱仪可以遵循变化。因此，CD光谱法适用于探测蛋白质（或其他大分子）结构或由于环境条件引起的结构的变化。 CD光谱已被广泛用于三种测量：识别蛋白质的结构，研究折叠和蛋白质折叠过程中的几何变化，并确定与结合相互作用和蛋白质展开有关的能量。本科生使用该光谱仪。在接受使用这种重要工具的培训期间，研究经验为学生提供了未来职业和医学和科学领域的高级学位的准备。 NSF资助的STEM学者计划还使用了该工具，该计划为具有对科学领域感兴趣的不同背景的学生提供了一种社区感。获得该光谱仪的获取为学生和教师开辟了新的研究道路，它可以通过跨学科项目帮助学生看到更多科学的相互联系。学生可以在课程中获得该乐器，最终导致研究人员准备进入研究生或科学中的职业。此奖励循环二分法光谱仪旨在增强各个级别的研究和教育，尤其是：（a）在：（a）在：（a）在小蛋白质中开发小蛋白质的钛和研究型dnna clastory（dna clate），（a）吸附的聚苯基 - 赖氨酸，以了解分子结构如何转化为对手性纳米颗粒组件的控制，以及（c）研究核小体的折叠，以洞悉核小体中的DNA如何与VIVO中的其他生物分子相互作用。

项目成果

DNA Cleavage by a De Novo Designed Protein–Titanium Complex

通过 De Novo 设计的蛋白质钛复合物进行 DNA 切割

• DOI: 10.1021/acs.inorgchem.0c01707
• 发表时间: 2020
• 期刊: Inorganic Chemistry
• 影响因子: 4.6
• 作者: Paredes, Alexander;Loh, Brittany M.;Peduzzi, Olivia M.;Reig, Amanda J.;Buettner, Katherine M.
• 通讯作者: Buettner, Katherine M.