NSF Postdoctoral Fellowship in Biology FY 2017: A large-scale, experimental test of local adaption in the eastern oyster along a natural salinity gradient in the Gulf of Mexico

2017 财年 NSF 生物学博士后奖学金：对墨西哥湾东部牡蛎沿自然盐度梯度的局部适应进行大规模实验测试

• 批准号: 1711319
• 负责人: Kevin Johnson
• 金额: $ 13.8万
• 依托单位: Johnson Kevin M
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1711319&HistoricalAwards=false
• 关键词: NSF; Postdoctoral; Fellowship; Biology; FY

This is an NSF Postdoctoral Research Fellowship in Biology, under the program Broadening Participation of Groups Under-represented in Biology. The fellow, Kevin Marquez Johnson, is conducting research and receiving training that is increasing the participation of groups underrepresented in biology. The fellow is being mentored by Dr. Morgan Kelly at Louisiana State University. The project is focused on learning how oysters from different regions in the Gulf of Mexico respond to variations in temperature and salinity. This work is important because changes in land-use and rainfall can dramatically influence salinity and temperature in the Gulf. By investigating how present-day populations respond to changes in temperature and salinity, this research will provide a valuable resource to conservation biologists, state managers, and small-scale farmers as they maintain over 2 million acres of public and private oyster grounds that produced 14.5 million pounds of oysters in 2015. This research explores adaptations to local environmental conditions at three oyster reefs located along a natural salinity gradient in the Gulf of Mexico. Evidence for adaptation to salinity and temperature is being assessed by measuring three indicators of individual oyster health: (i) food ingestion rate, (ii) growth rate, and (iii) the heritability of environmentally-induced epigenetic modifications. Utilizing classic metrics for oyster health (i.e. ingestion and growth rates) across multiple generations will provide the necessary tools to determine if oysters from these three reefs are locally adapted to their native environment. This research also uses epigenomic sequencing to track DNA methylation patterns from the parent population (F0) to the second generation (F2). Tracking these environmentally inducible epigenomic changes across multiple generations is providing valuable insight into the heritability of these markers and quantifying the variation of these markers in natural populations. Finally, this work is broadeningparticipation of under-represented groups in biology at both Louisiana State University (LSU) and local high schools. The fellow is mentoring LSU undergraduate students and encouraging them to participate in the Louis Stokes Louisiana Alliance for Minority Participation (LS-LAMP) program. The fellow also is working with eight local high school teacher and student pairs to establish a summer education course in bioinformatics, thereby communicating the utility of these tools in the natural sciences.

这是美国国家科学基金会生物学博士后研究奖学金，属于“扩大生物学领域代表性不足群体的参与”计划。凯文·马克斯·约翰逊 (Kevin Marquez Johnson) 研究员正在进行研究并接受培训，以提高生物学领域代表性不足的群体的参与度。该研究员受到路易斯安那州立大学摩根·凯利博士的指导。该项目的重点是了解墨西哥湾不同地区的牡蛎如何应对温度和盐度的变化。这项工作很重要，因为土地利用和降雨量的变化会极大地影响海湾的盐度和温度。通过调查当今人口如何应对温度和盐度的变化，这项研究将为保护生物学家、州管理者和小规模养殖户提供宝贵的资源，因为他们维护着超过 200 万英亩的公共和私人牡蛎养殖场，生产了 14.5 2015 年生产了 100 万磅牡蛎。这项研究探讨了墨西哥湾自然盐度梯度沿线的三个牡蛎礁对当地环境条件的适应。通过测量个体牡蛎健康的三个指标来评估适应盐度和温度的证据：（i）食物摄入率，（ii）生长率，以及（iii）环境引起的表观遗传修饰的遗传力。利用多代牡蛎健康的经典指标（即摄入量和生长率）将为确定这三个珊瑚礁的牡蛎是否适应其原生环境提供必要的工具。这项研究还使用表观基因组测序来追踪从亲代群体 (F0) 到第二代 (F2) 的 DNA 甲基化模式。追踪这些环境诱导的多代表观基因组变化，可以为了解这些标记的遗传性提供有价值的见解，并量化这些标记在自然群体中的变异。最后，这项工作正在扩大路易斯安那州立大学 (LSU) 和当地高中生物学领域代表性不足的群体的参与。该研究员正在指导路易斯安那州立大学本科生，并鼓励他们参加路易斯斯托克斯路易斯安那州少数族裔参与联盟 (LS-LAMP) 计划。该研究员还与当地八名高中教师和学生合作，开设生物信息学暑期教育课程，从而交流这些工具在自然科学中的实用性。

项目成果

Population epigenetic divergence exceeds genetic divergence in the Eastern oyster Crassostrea virginica in the Northern Gulf of Mexico

墨西哥湾北部的东部牡蛎Crassostrea virginica的种群表观遗传差异超过了遗传差异

• DOI: 10.1111/eva.12912
• 发表时间: 2020-01-07
• 期刊: Evolutionary Applications
• 影响因子: 4.1
• 作者: Kevin M. Johnson;M. Kelly
• 通讯作者: M. Kelly

Synergistic Effects of Temperature and Salinity on the Gene Expression and Physiology of Crassostrea virginica

温度和盐度对弗吉尼亚巨牡蛎基因表达和生理的协同效应

• DOI: 10.1093/icb/icz035
• 发表时间: 2019-05
• 期刊: Integrative and Comparative Biology
• 影响因子: 2.6
• 作者: Jones, H. R.;Johnson, K. M.;Kelly, M. W.
• 通讯作者: Kelly, M. W.