SusChEM: GOALI: Harnessing the Antimicrobial Properties of Copper to Control Legionella in Plumbing Systems

SusChEM：目标：利用铜的抗菌特性来控制管道系统中的军团菌

• 批准号: 1706733
• 负责人: Marc Edwards
• 金额: $ 37.98万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1706733&HistoricalAwards=false
• 关键词: SusChEM; GOALI; Harnessing; Antimicrobial; Properties

Proposal Number: 1706733PI Name: Marc A. Edwards The growing public health threat in drinking water from opportunistic pathogens (OPs), such as Legionella, calls for practical engineering solutions that are compatible with sustainability goals. Taking advantage of the natural disinfecting properties of copper (Cu) pipes, which are widely used in plumbing systems, is an attractive approach for control of Legionella; however, Cu is not consistently effective in practice, as it sometimes inhibits and other times stimulates Legionella growth. This research seeks to address this fundamental knowledge gap through bench, pilot, and field-scale studies to identify the water chemistries that enhance or inhibit Cu-driven disinfection of Legionella. This research is potentially transformative as it will inform practical, economical, and sustainable means of addressing a key public health threat just as trillions of dollars will be invested to upgrade antiquated water infrastructure.The overarching hypothesis for this research is that water chemistry directly and indirectly influences Cu toxicity towards Legionella. The specific objectives are to: 1) Determine the effects of Cu speciation, complexation, and precipitation in governing toxicity towards Legionella under conditions relevant to hot water building plumbing systems; 2) Explore the role of microbial communities inhabiting plumbing systems to mediate toxicity of Cu, e.g., through shielding or responding to nutrients released from corrosion reactions (e.g., H2 evolved), and confirm Objective 1 phenomena under realistic pilot-scale plumbing conditions; and 3) Conduct a field survey to validate relationships among physicochemical, Cu speciation/solubility, and microbial factors in real-world plumbing systems and illuminate drivers of Cu-enhanced versus Cu-inhibited Legionella growth. This study will be the first systematic examination of the speciation- and solubility-dependent nature of Cu toxicity towards Legionella in potable water systems, particularly within hot water that is most relevant to OP proliferation. Of particular interest is the occurrence and behavior of cuprous (Cu1+) ions. Cu1+ is probably non-toxic to Legionella and may even indirectly stimulate its growth by catalyzing deposition corrosion of less noble metal plumbing materials which will then provide H2 for autotrophic hydrogen-oxidizing bacteria. Cupric (Cu2+) ions have strong biocidal properties, but effectiveness may be limited by temperature, redox, inorganic and organic ligands, corrosion control agents, and biofilm properties. An innovative combination of tools will be used to characterize complex interdependencies among the microbial community composition, physicochemical properties, Cu speciation, and Legionella, including next-generation DNA sequencing, green-fluorescence protein modified strains, flow cytometry, scanning electron microscopy, and x-ray diffraction crystallography. Knowledge generated by this research has the potential to guide building maintenance practices to prevent the proliferation of Legionella.

提案编号：1706733PI 姓名：Marc A. Edwards 军团菌等机会性病原体 (OP) 对饮用水造成的公共卫生威胁日益严重，需要与可持续发展目标相兼容的实用工程解决方案。利用广泛用于管道系统的铜 (Cu) 管的天然消毒特性，是控制军团菌的一种有吸引力的方法；然而，铜在实践中并不总是有效，因为它有时会抑制军团菌的生长，有时会刺激军团菌的生长。 本研究旨在通过实验室、中试和现场规模研究来解决这一基本知识差距，以确定增强或抑制铜驱动的军团菌消毒的水化学物质。这项研究具有潜在的变革性，因为它将提供解决关键公共卫生威胁的实用、经济和可持续的方法，就像将投资数万亿美元来升级陈旧的水基础设施一样。这项研究的总体假设是水化学直接和间接影响铜对军团菌的毒性。具体目标是： 1) 确定铜形态、络合和沉淀在与热水建筑管道系统相关的条件下控制军团菌毒性的影响； 2) 探索管道系统中的微生物群落在介导铜毒性方面的作用，例如通过屏蔽或响应腐蚀反应释放的营养物质（例如释放出的 H2），并在实际的中试规模管道条件下确认目标 1 现象； 3) 进行现场调查，以验证现实世界管道系统中物理化学、铜形态/溶解度和微生物因素之间的关系，并阐明铜增强与铜抑制军团菌生长的驱动因素。 这项研究将首次系统地研究铜对饮用水系统中军团菌的毒性的形态和溶解度依赖性，特别是在与 OP 增殖最相关的热水中。特别令人感兴趣的是亚铜 (Cu1+) 离子的出现和行为。 Cu1+可能对军团菌无毒，甚至可以通过催化次贵金属管道材料的沉积腐蚀来间接刺激其生长，然后为自养氢氧化细菌提供氢气。铜 (Cu2+) 离子具有很强的杀菌特性，但有效性可能受到温度、氧化还原、无机和有机配体、腐蚀控制剂和生物膜特性的限制。将使用创新的工具组合来表征微生物群落组成、理化特性、铜形态和军团菌之间复杂的相互依赖性，包括下一代 DNA 测序、绿色荧光蛋白修饰菌株、流式细胞术、扫描电子显微镜和 x射线衍射晶体学。这项研究产生的知识有可能指导建筑维护实践，以防止军团菌的扩散。

项目成果

Natural Organic Matter, Orthophosphate, pH, and Growth Phase Can Limit Copper Antimicrobial Efficacy for Legionella in Drinking Water

天然有机物、正磷酸盐、pH 值和生长阶段会限制铜对饮用水中军团菌的抗菌功效

• DOI: 10.1021/acs.est.0c06804
• 发表时间: 2021-02
• 期刊: Environmental Science & Technology
• 影响因子: 11.4
• 作者: Song, Yang;Pruden, Amy;Edwards, Marc A.;Rhoads, William J.
• 通讯作者: Rhoads, William J.

Replicable simulation of distal hot water premise plumbing using convectively-mixed pipe reactors

使用对流混合管反应器对远端热水管道进行可复制模拟

• DOI: 10.1371/journal.pone.0238385
• 发表时间: 2020-09-16
• 期刊: PLoS ONE
• 影响因子: 3.7
• 作者: M. S. Spencer;Abraham Cullom;W. Rhoads;A. Pruden;Marc A Edwards
• 通讯作者: Marc A Edwards

Critical Review: Propensity of Premise Plumbing Pipe Materials to Enhance or Diminish Growth of Legionella and Other Opportunistic Pathogens

批判性评论：室内管道材料增强或减少军团菌和其他机会病原体生长的倾向

• DOI: 10.3390/pathogens9110957
• 发表时间: 2020-11-17
• 期刊: Pathogens
• 影响因子: 3.7
• 作者: Cullom AC;Martin RL;Song Y;Williams K;Williams A;Pruden A;Edwards MA
• 通讯作者: Edwards MA

Shotgun Metagenomics Reveals Taxonomic and Functional Shifts in Hot Water Microbiome Due to Temperature Setting and Stagnation

鸟枪法宏基因组学揭示了由于温度设定和停滞导致的热水微生物组的分类和功能变化

• DOI: 10.3389/fmicb.2018.02695
• 发表时间: 2018-11-13
• 期刊: Frontiers in Microbiology
• 影响因子: 5.2
• 作者: D. Dai;W. Rhoads;M. Edwards;A. Pruden
• 通讯作者: A. Pruden

Interactive Effects of Copper Pipe, Stagnation, Corrosion Control, and Disinfectant Residual Influenced Reduction of Legionella pneumophila during Simulations of the Flint Water Crisis

弗林特水危机模拟过程中铜管、停滞、腐蚀控制和消毒剂残留的相互作用影响嗜肺军团菌的减少

• DOI: 10.3390/pathogens9090730
• 发表时间: 2020-09-04
• 期刊: Pathogens
• 影响因子: 3.7
• 作者: Martin RL;Strom OR;Pruden A;Edwards MA
• 通讯作者: Edwards MA