Nanofluidic Charge Coupled Devices for Molecular Separation and Sensing

用于分子分离和传感的纳流体电荷耦合器件

• 批准号: 1710831
• 负责人: Weihua Guan
• 金额: $ 34.67万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1710831&HistoricalAwards=false
• 关键词: Nanofluidic; Charge; Coupled; Devices; Molecular; Nanofluidic; Charge; Coupled; Devices; Molecular

Molecular separation is at the heart of molecular diagnosis and analyzing molecular biomarkers in genomics and proteomics studies. Gel and capillary electrophoresis are two dominant molecule separation technologies based on charge/size dispersion. The research objective of this proposal is to explore a novel nanofluidic molecular charge-coupled device (CCD) suitable for integrated electro-fluidic molecular separation and sensing, fundamentally different from the conventional gel and capillary electrophoresis. The proposed nanofluidic molecular CCD is based on the unique field effect coupling in nanofluidic systems, in which new transport behavior and functionality can be developed by leveraging the unique electrostatic coupling at the nanometer. The success of the proposed project will transform the biomolecule sensing and separation by exploring the rich properties of the novel nanofluidic molecular CCD. The project also includes education and outreach activities to tightly integrate the research efforts and results with graduate, undergraduate, and K-12 education and to globally disseminate both research and the education outcomes.Molecule transport at nanometer scale plays an important role in many biological, chemical, physical and engineering systems. Nanoscale channels offer a unique platform to explore new phenomena appearing for molecule confined in nanometers scales. New transport behavior and functionalities can be developed by taking advantage of the specific couplings occurring at these scales. Despite the fact that CCD image sensors have been indispensable tools for a variety of applications in various scientific and engineering disciplines, a similar bucket brigade transport and analysis of charged biomolecules in nanochannels has not been explored and realized. The proposed research will explore the field effect controlled bucket brigade transport of charged molecules by rational design, simulation, fabrication, and validation of a novel nanofluidic molecule CCD device. The outcome of the research will enable a paradigm shift in controllable molecule transport and separation. The major challenges in realizing a CCD-like device for bucket brigade transport and analysis are the lack of fundamental understanding of the dynamic electric field coupling to the charged molecules at the nanoscale, and the lack of feasible top-down engineered fabrication methods to produce the metal-insulator-nanochannel (MIN) structure. The proposed research objectives will be achieved through the following three aims: (i) Understand the device physics of nanofluidic molecular CCD, (ii) Explore viable device fabrication and integration techniques, and (iii) Investigate nanofluidic molecular CCDs for biomolecule transport and separation.

分子分离是分子诊断和分析基因组学和蛋白质组学研究中的分子生物标志物的核心。凝胶和毛细管电泳是基于电荷/尺寸分散体的两种主要分子分离技术。该提案的研究目标是探索一种新型的纳米流体分子电荷偶联装置（CCD），适用于集成的电富集分子分离和传感，这与常规的凝胶和毛细血管电泳根本不同。提出的纳米流体分子CCD基于纳米流体系统中独特的场效应耦合，在该偶联中，可以通过利用纳米计的唯一静电耦合来开发新的传输行为和功能。拟议项目的成功将通过探索新型纳米流体分子CCD的丰富特性来改变生物分子的感应和分离。该项目还包括教育和外展活动，以将研究工作和结果与研究生，本科生和K-12教育紧密整合，并在全球范围内传播研究和教育成果。纳米量表的 - 分子运输在许多生物学，化学，物理和工程系统中都起着重要作用。纳米级通道提供了一个独特的平台，以探索限制在纳米尺度的分子出现的新现象。可以通过利用在这些量表下发生的特定耦合来开发新的运输行为和功能。尽管CCD图像传感器已成为各种科学和工程学科中各种应用的必不可少的工具，但尚未探索并实现类似的纳米渠道中带电的生物分子的类似的桶形旅和分析。拟议的研究将探索通过合理设计，模拟，制造和验证新型纳米流体分子CCD设备的现场效应控制的铲斗旅的运输。研究的结果将使可控分子传输和分离的范式转移。实现用于铲斗旅传输和分析的类似于CCD的设备的主要挑战是对纳米级的动态电场耦合的基本了解缺乏，缺乏可行的自上而下的工程制造方法，无法生产金属绝缘仪 - 荷兰 - 荷兰纳米棒（min）结构。提出的研究目标将通过以下三个目的实现：（i）了解纳米流体分子CCD的装置物理学，（ii）探索可行的装置制造和集成技术，以及（iii）研究纳米荧光分子CCD的生物分子运输和分离。