Interactions of Biomolecules and Bacteria with Titanium at the Mineral Microbe Frontier

矿物微生物前沿生物分子和细菌与钛的相互作用

• 批准号: 1708793
• 负责人: Ann Valentine
• 金额: $ 33万
• 依托单位: Temple University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1708793&HistoricalAwards=false
• 关键词: Interactions; Biomolecules; Bacteria; Titanium; Mineral

This award is funded by the Environmental Chemical Sciences Program in the Division of Chemistry. Professor Ann M. Valentine of Temple University is supported to explore the interaction of titanium oxide surfaces with metal-scavenging biomolecules and/or microorganisms. Titanium is very abundant both in the environment and in organisms, and increasingly used in consumer products and industrial applications. Titanium is not widely appreciated as a bioactive element as it has a reputation for extreme inertness and insolubility in water. This research examines the ability of titanium dioxide to stick to (adhere) and release from well-characterized biomolecules and from a bacterium. Iron-scavenging biomolecules bind titanium especially tightly and keep it soluble and stable. They can also cause the release of large quantities of titanium ions into solution. This research sheds light on the impact of microorganisms on the environmental chemistry of titanium and reveals the biological role of titanium. The research is integrated with a new outreach program for rising high school juniors called Philadelphia Environmental Chemistry Explorations (PECE). Environmental bioinorganic chemistry is used as a platform to broaden participation in science. This project focuses on the interactions of iron-scavenging siderophores and bacteria with titanium dioxide (TiO2) surfaces. This inquiry sits at the intersection of inorganic and bioinorganic chemistry, biogeochemistry, and materials and environmental chemistry. Siderophores bind strongly to TiO2, and some siderophores dissolve titanium ions from the metal oxide surface, significantly remodeling the mineral in the process. Electronic spectroscopy, elemental analysis, and mass spectrometry reveal metal ion concentrations and speciation in solution. The oxide surface and properties before, during, and after dissolution are being probed using Attenuated Total Reflectance-Fourier Transfer Infrared (ATR-FTIR) as well as microscopy and related techniques. The mutual interference of titanium and iron is a special focus. Microbe/oxide and biomolecule/oxide interactions are being studied using the bacterium Rhodococcus ruber GIN-1, which adheres to titanium dioxide and incorporates significant quantities of titanium into its biomass. Titanium dioxide surface binding proteins are being identified and characterized. Their abundance and properties are correlate to whole-organism phenomena. Titanium uptake is being quantified and intracellular titanium-binding molecules isolated. A research program is maintained that educates and trains undergraduates and graduate students at the interface of inorganic chemistry and biochemistry. Research activities are integrated with chemistry outreach and with a new program for rising high school juniors. This program uses environmental bioinorganic chemistry as a platform to broaden participation in science.

该奖项由化学部环境化学科学计划资助。坦普尔大学的Ann M. Valentine教授得到了支持，以探索氧化钛表面与金属扫除生物分子和/或微生物的相互作用。钛在环境和生物中都非常丰富，并且越来越多地用于消费产品和工业应用中。 钛并未被广泛视为生物活性元素，因为它因极度惰性和对水的不溶性而闻名。这项研究研究了二氧化钛坚持（粘附）并从特征良好的生物分子和细菌中释放的能力。铁扫除生物分子特别紧密地结合钛，并使其可溶和稳定。它们还可以使大量钛离子释放到溶液中。这项研究阐明了微生物对钛环境化学的影响，并揭示了钛的生物学作用。这项研究与新的高中大三学生推广计划融合在一起，称为费城环境化学探索（PECE）。环境生物无机化学被用作扩大参与科学的平台。该项目侧重于铁扫描的铁载体和细菌与二氧化钛（TIO2）表面的相互作用。该查询是无机和生物无机化学，生物地球化学以及材料和环境化学的交集。铁载体与TiO2强烈结合，一些铁载体将钛离子从金属氧化物表面溶解，从而在过程中显着重塑了矿物质。电子光谱，元素分析和质谱法揭示了溶液中的金属离子浓度和物种形成。使用衰减的总反射率转移红外（ATR-FTIR）以及显微镜和相关技术，对氧化物表面和特性进行了探测。钛和铁的相互干扰是一个特别的焦点。使用细菌Ruber Gin-1研究了微生物/氧化物和生物分子/氧化物相互作用，该细菌粘附在二氧化钛上，并在其生物量中掺入大量钛。正在鉴定和表征二氧化钛表面结合蛋白。 它们的丰度和特性与全生物现象相关。钛摄取正在被定量，细胞内结合分子分离出来。坚持一项研究计划，该计划在无机化学和生物化学的界面上教育和培训本科生和研究生。研究活动与化学外展和新的高中大三学生计划融合在一起。该计划使用环境生物有机化学作为扩大参与科学的平台。

项目成果

Photochemistry of ferritin decorated with plasmonic gold nanoparticles

• DOI: 10.1039/c8en01000e
• 发表时间: 2019-01
• 期刊: Environmental Science: Nano
• 作者: Elizabeth B. Cerkez;K. Dutton;Y. Ghidey;Mark A. Kukulka;A. Valentine;D. Strongin

TiO 2 exposure alters transition metal ion quota in Rhodococcus ruber GIN-1

TiO 2 暴露改变红球菌 GIN-1 中的过渡金属离子配额

• DOI: 10.1039/c9mt00305c
• 发表时间: 2020
• 期刊: Metallomics
• 影响因子: 3.4
• 作者: Gallo, Annastassia D.;Zierden, Mark R.;Profitt, Lauren A.;Jones, Kayleigh E.;Bonafide, Christopher P.;Valentine, Ann M.
• 通讯作者: Valentine, Ann M.