DISSERTATION RESEARCH: The Evolution of Plant Drought Tolerance and Gene Function Across Historic Frequency Gradients

论文研究：植物耐旱性和基因功能在历史频率梯度上的演变

基本信息

批准号：
1701918
负责人：
John McKay
金额：
$ 1.98万
依托单位：
Colorado State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-06-01 至 2019-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1701918&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Evolution Plant Drought

项目摘要

This research will investigate the function of genes possibly involved in drought adaptation in wild populations of the plant Arabidopsis thaliana. Droughts are one of nature?s most devastating forces, affecting millions of people each year. Yet as agents of strong selection on plant populations, they are also valuable for understanding basic evolutionary processes, such as discovering the genes involved in adaptation. Furthermore, studying plant evolution in the context of drought may produce the critical knowledge needed to develop drought tolerant crops. Using a global map of historic drought frequency based on remote sensing data, populations were identified that experience very different drought frequencies. From data on genetic differences among those populations, 20 genes were identified that will be studied for their molecular and phenotypic effects. This work is expected to yield key insight into the evolution of gene function, and to confirm genes underlying drought tolerance traits. The results of studying these genes may be of great value to breeding programs working to improve drought tolerance in crop species. In addition, the project will involve mentoring both undergraduate and high school students, including individuals from groups that are underrepresented in the sciences. Historic drought frequency significantly predicts important drought tolerance traits, flowering time and water use efficiency, among natural accessions of Arabidopsis thaliana. The project will investigate the effects of loss of function alleles on 20 genes involved in flowering time or water use efficiency. A reverse genetics approach based on T-DNA gene knockouts will be used to artificially induce mutations in these genes in a common genomic background. The project will quantify the effect sizes of these hypothesized genes on flowering time and water use efficiency. It will also characterize effects on whole genome expression profiles and abiotic signaling cascades using RNAseq.

这项研究将研究植物拟南芥野生种群中可能参与干旱适应的基因的功能。干旱是自然界中最具毁灭性的力量之一，每年影响数百万人。然而，作为对植物种群进行强烈选择的代理人，它们也对理解基本进化过程（例如发现适应性的基因）也很有价值。此外，在干旱的背景下研究植物的进化可能会产生开发耐旱作物所需的关键知识。使用基于遥感数据的全球历史干旱频率地图，确定种群经历了截然不同的干旱频率。从这些人群之间的遗传差异数据中，发现了20个基因，这些基因将研究其分子和表型作用。预计这项工作将产生对基因功能演变的关键见解，并确认耐旱性特征的基因。研究这些基因的结果对于繁殖计划旨在提高农作物物种的干旱耐受性具有很高的价值。此外，该项目将涉及指导本科生和高中生，包括来自科学中人数不足的团体的个人。历史干旱频率显着预测了拟南芥的自然加入中，重要的干旱耐受性特征，开花时间和用水效率。该项目将研究功能等位基因丧失对花费时间或用水效率的20个基因的影响。基于T-DNA基因敲除的反向遗传学方法将用于在共同的基因组背景中人为地诱导这些基因中的突变。该项目将量化这些假设基因对开花时间和用水效率的效果大小。它还将使用RNASEQ对整个基因组表达谱和非生物信号级联反应的影响。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Drought adaptation in Arabidopsis thaliana by extensive genetic loss-of-function

DOI：
10.7554/elife.41038
发表时间：
2018-12
期刊：
eLife
影响因子：
7.7
作者：
J. G. Monroe;T. Powell;Nicholas Price;J. Mullen;A. Howard;Kyle Evans;J. Lovell;J. McKay
通讯作者：
J. G. Monroe;T. Powell;Nicholas Price;J. Mullen;A. Howard;Kyle Evans;J. Lovell;J. McKay

Drought regimens predict life history strategies in Heliophila

干旱方案预测Heliophila的生活史策略

DOI：
10.1111/nph.15919
发表时间：
2019
期刊：
New Phytologist
影响因子：
9.4
作者：
Monroe, J. Grey;Gill, Brian;Turner, Kathryn G.;McKay, John K.
通讯作者：
McKay, John K.

Convergent Loss of an EDS1/PAD4 Signaling Pathway in Several Plant Lineages Reveals Coevolved Components of Plant Immunity and Drought Response

DOI：
10.1105/tpc.19.00903
发表时间：
2020-07-01
期刊：
PLANT CELL
影响因子：
11.6
作者：
Baggs, Erin L.;Monroe, J. Grey;Krasileva, Ksenia, V
通讯作者：
Krasileva, Ksenia, V

AraPheno and the AraGWAS Catalog 2020: a major database update including RNA-Seq and knockout mutation data for Arabidopsis thaliana

DOI：
10.1093/nar/gkz925
发表时间：
2020-01-08
期刊：
NUCLEIC ACIDS RESEARCH
影响因子：
14.9
作者：
Togninalli, Matteo;Seren, Uemit;Grimm, Dominik G.
通讯作者：
Grimm, Dominik G.

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John McKay其他文献

OA-01 Talquetamab (tal) + Daratumumab (dara) + Pomalidomide (pom) in Patients (pts) With Relapsed/Refractory Multiple Myeloma (RRMM): Results from the Phase 1b TRIMM-2 Study

DOI：
10.1016/s2152-2650(24)01842-1
发表时间：
2024-09-01
期刊：
Conference abstract
影响因子：
作者：
Nizar J. Bahlis;Niels van de Donk;Donna Reece;Manisha Bhutani;Bhagirathbhai Dholaria;Anita D'Souza;Thomas Martin;John McKay;Alfred Garfall;Amrita Krishnan;Kalpana Bakshi;Lijuan Kang;Lien Vandenberk;Thomas Prior;Jaszianne Tolbert;Ajai Chari
通讯作者：
Ajai Chari

Characterizing a Cohort of Patients with Hemophilia B Treated with Fidanacogene Elaparvovec from the Phase 3 Benegene-2 Study Who Returned to Factor IX Prophylaxis

DOI：
10.1182/blood-2023-181223
发表时间：
2023-11-02
期刊：
Conference abstract
影响因子：
作者：
Laurent Frenzel;Kaan Kavakli;Robert Klamroth;Shyh-Shin Chiou;Amy D. Shapiro;Pengling Sun;Joanne Fuiman;John McKay;Annie F. Fang;Francesca Biondo;Frank Plonski;Jeremy Rupon
通讯作者：
Jeremy Rupon

Finite Simple Groups: Thirty Years of the Atlas and Beyond

有限简单群：阿特拉斯三十年及以后

DOI：
10.1090/conm/694
发表时间：
2014
期刊：
Communications in Algebra
影响因子：
0.7
作者：
Yang;John McKay
通讯作者：
John McKay

P-014 Efficacy and Safety of ABBV-383, a BCMA Bispecific Antibody, in Black Patients With Relapsed/Refractory Multiple Myeloma: A Subgroup Analysis of a Phase 1 Trial

DOI：
10.1016/s2152-2650(24)01917-7
发表时间：
2024-09-01
期刊：
Conference abstract
影响因子：
作者：
Anita D'Souza;Cesar Rodriguez-Valdes;Shaji Kumar;Alfred Chung;Sascha Tuchman;Hana Safah;Katja Weisel;Raphael Teipel;Neha Korde;Ravi Vij;Orlando Bueno;Tanya Rosenberg;Rajvineeth Pothacamury;Akshanth Polepally;Aarif Ahsan;Shane Lee;Ziyi Jin;Shelli Spence;Chetasi Talati;John McKay
通讯作者：
John McKay

Use of Fidanacogene Elaparvovec, a Gene Therapy Vector, to Deliver a Stable, Fully Functional Human Factor IX Transgene for the Treatment of Hemophilia B: A Combined Analysis of Safety

DOI：
10.1182/blood-2024-193453
发表时间：
2024-11-05
期刊：
Conference abstract
影响因子：
作者：
Ben Samelson-Jones;Laurent Frenzel;Kaan Kavakli;Adam Cuker;Jerome M. Teitel;Pengling Sun;Lisa J Wilcox;Francesca Biondo;Benjamin Hutter;John McKay;Delphine Agathon;Frank Plonski
通讯作者：
Frank Plonski