CAREER: Triggered Energy Transfer Chemiluminescence for In Vivo Imaging

职业：用于体内成像的触发能量转移化学发光

• 批准号: 1653474
• 负责人: Alexander Lippert
• 金额: $ 61.16万
• 依托单位: Southern Methodist University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1653474&HistoricalAwards=false
• 关键词: CAREER; Triggered; Energy; Transfer; Chemiluminescence

In this CAREER project funded by the Chemical Structure, Dynamics & Mechanisms B Program of the Chemistry Division, Professor Alexander R. Lippert of the Department of Chemistry at Southern Methodist University is developing responsive glow stick chemistry (chemiluminescence) for biological imaging. The project aims to synthesize and understand specialized molecules that spontaneously emit light in response to biological events. Mastering this fundamental chemical phenomenon found in fireflies and jellyfish provides new methods to measure and visualize the chemistry that lies at the heart of biological processes. The visual appeal of multi-colored chemiluminescence will be used to inspire future generations of students from K-12 to the graduate level with a centralized outreach theme of encouraging scientific creativity through seminars, K-12 research opportunities, and coursework aimed at teaching the creative process in a scientific context.Triggered chemiluminescence has the potential to dramatically transform biological imaging in wild-type organisms by eliminating autofluorescence, photobleaching, and other issues endemic to traditional fluorescence imaging techniques, but fundamental advances in understanding how molecular structure affects chemiluminescence emission is required. This project aims to synthesize, characterize, and understand new classes of triggered chemiluminescent imaging agents structurally designed to manipulate energy transfer processes. Fundamental questions to be addressed include: 1) How can the energy in a chemiluminescent excited state be efficiently transferred by through-space and through-bond energy transfer mechanisms to enhance luminescence emission? 2) What molecular structures will enable tuning of emission wavelengths for near-infrared and ratiometric readouts? 3) How can chemiluminescence emission be dynamically triggered with spatiotemporally controlled illumination to maximize signal to background in a region of interest?

在这个由化学系化学结构、动力学和机制 B 项目资助的职业项目中，南卫理公会大学化学系的 Alexander R. Lippert 教授正在开发用于生物成像的响应式荧光棒化学（化学发光）。该项目旨在合成和理解能够响应生物事件而自发发光的特殊分子。掌握萤火虫和水母中发现的这种基本化学现象，为测量和可视化生物过程核心化学物质提供了新方法。多色化学发光的视觉吸引力将用于激励未来几代从 K-12 到研究生水平的学生，其中心外展主题是通过研讨会、K-12 研究机会和旨在教授创造性的课程来鼓励科学创造力。触发化学发光有可能通过消除自发荧光、光漂白和传统荧光成像技术特有的其他问题来显着改变野生型生物体的生物成像，但在理解分子结构如何影响方面取得了根本性进展需要化学发光发射。该项目旨在合成、表征和理解新型触发化学发光成像剂，其结构设计用于操纵能量转移过程。需要解决的基本问题包括：1）化学发光激发态的能量如何通过空间和键合能量转移机制有效转移以增强发光发射？ 2) 哪些分子结构能够调节近红外和比率读数的发射波长？ 3) 如何通过时空控制照明动态触发化学发光发射，以最大化感兴趣区域中的背景信号？

项目成果

Ultrasensitive Chemiluminescent Detection of Cathepsin B: Insights into the New Frontier of Chemiluminescent Imaging

组织蛋白酶 B 的超灵敏化学发光检测：深入了解化学发光成像新领域

• DOI: 10.1002/anie.201711228
• 发表时间: 2017-11
• 期刊: Angewandte Chemie International Edition
• 作者: Ryan, Lucas S.;Lippert, Alexander R.
• 通讯作者: Lippert, Alexander R.

A Chemiluminescent Probe for HNO Quantification and Real‐Time Monitoring in Living Cells

用于活细胞中 HNO 定量和实时监测的化学发光探针

• DOI: 10.1002/anie.201811257
• 发表时间: 2018-12
• 期刊: Angewandte Chemie International Edition
• 作者: An, Weiwei;Ryan, Lucas S.;Reeves, Audrey G.;Bruemmer, Kevin J.;Mouhaffel, Lyn;Gerberich, Jeni L.;Winters, Alexander;Mason, Ralph P.;Lippert, Alexander R.
• 通讯作者: Lippert, Alexander R.

Chemiluminescent spiroadamantane-1,2-dioxetanes: Recent advances in molecular imaging and biomarker detection

化学发光螺金刚烷-1,2-二氧杂环丁烷：分子成像和生物标志物检测的最新进展

• DOI: 10.1016/j.cbpa.2022.102134
• 发表时间: 2022-06
• 期刊: Current Opinion in Chemical Biology
• 影响因子: 7.8
• 作者: Kagalwala, Husain N.;Reeves, R. Tate;Lippert, Alexander R.
• 通讯作者: Lippert, Alexander R.

Chemiluminescent 1,2‐Dioxetane Iridium Complexes for Near‐Infrared Oxygen Sensing

用于近红外氧传感的化学发光 1,2—二氧杂环丁烷铱配合物

• DOI: 10.1002/anie.202115704
• 发表时间: 2022-03-14
• 期刊: Angewandte Chemie International Edition
• 作者: Kagalwala HN;Gerberich J;Smith CJ;Mason RP;Lippert AR
• 通讯作者: Lippert AR

Ratiometric pH Imaging Using a 1,2-Dioxetane Chemiluminescence Resonance Energy Transfer Sensor in Live Animals

使用 1,2-二氧杂环丁烷化学发光共振能量转移传感器对活体动物进行比率 pH 成像

• DOI: 10.1021/acssensors.0c01393
• 发表时间: 2020-09
• 期刊: ACS Sensors
• 影响因子: 8.9
• 作者: Ryan, Lucas S.;Gerberich, Jeni;Haris, Uroob;Nguyen, Daphne;Mason, Ralph P.;Lippert, Alexander R.
• 通讯作者: Lippert, Alexander R.