Hybrid Bioprinting of Regenerative Osteochondral (Bone-Cartilage) Tissues

再生骨软骨（骨软骨）组织的混合生物打印

基本信息

批准号：
1663128
负责人：
Salil Desai
金额：
$ 30万
依托单位：
North Carolina Agricultural & Technical State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-10-01 至 2023-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1663128&HistoricalAwards=false
关键词：
Hybrid Bioprinting Regenerative Osteochondral Bone

项目摘要

Regenerative tissue engineering combines medicine and engineering to develop custom tissue structures that replace damaged tissue or organs. Over the last two decades, research efforts have focused on the manufacture of tissue structures by combining cells and biological nutrients. However, many of these methods have limitations in their ability to precisely arrange cells and nutrients as in natural tissue structures. To address these issues, this research plans to investigate a hybrid bioprinting process, which can control the three dimensional organization of cells and trigger specific cellular activities. Cells from rat bone marrow will placed at target locations within a scaffold structure. They will be further transformed into bone and cartilage cell types by adjusting the bio printing process parameters and material compositions. If successful, the research will be the first step in the production of bone-tissue constructs, which could improve the quality of life for osteoarthritic patients, sports injury athletes and accident trauma survivors. Education and outreach efforts includes development of biomanufacturing coursework that will impact underrepresented students at both undergraduate and graduate levels. The PI will offer a summer camp for high-school students on biofabrication at the STEM Early College within Guilford County Schools. In addition, students will receive exposure to laboratories and pre-clinical trials at the Wake Forest Institute for Regenerative Medicine. This research will investigate hybrid bioprinting to control underlying pattern topology, mechanical stimuli and release of biochemical agents for cell based regenerative tissue engineering. The objectives of this research include: (1) computational modeling of the hybrid bioprinting process using finite element analysis and molecular dynamics models; (2) experimental investigation of different topological patterns, mechanical stimuli and biochemical cues on cell-scaffold interactions; (3) response surface optimization to establish relationships among interacting process parameters of hybrid bioprinting processes for optimal tissue engineering. The differentiation of rat cells into osteogenic (bone) and chondrogenic (cartilage) lineages will be studied within vascularized hydrogel scaffolds for long-term viability. The ability to manipulate bioprinting parameters and biomaterial compositions will create an effective method to building biomimetic functionally-gradient topographies. A model system will be developed to investigate the relationship between placement proximity, mechanical loading and release kinetics of bioactive factors on cell proliferation. Finally, biochemical assays and characterization of the regenerated bone-cartilage tissues will give insight for neo-tissue regeneration.

再生组织工程将医学和工程学结合起来，开发替代受损组织或器官的定制组织结构，研究工作主要集中在通过结合细胞和生物营养素来制造组织结构。为了解决这些问题，本研究计划研究一种混合生物打印过程，该过程可以控制细胞的三维组织并触发来自大鼠骨髓的细胞的特定细胞活动。将被放置在脚手架结构内的目标位置。如果成功，该研究将通过调整生物打印工艺参数和材料成分进一步转化为骨和软骨细胞类型，这将是生产骨组织结构的第一步，这可以改善骨关节炎患者的生活质量。、运动损伤运动员和事故创伤幸存者的教育和推广工作包括开发生物制造课程，这将影响本科和研究生阶段的代表性不足的学生。PI 将为高中生提供生物制造夏令营。此外，吉尔福德县学校内的 STEM 早期学院还将让学生接触维克森林再生医学研究所的实验室和临床前试验，这项研究将通过混合生物打印来控制潜在的图案拓扑、机械刺激和生化制剂的释放。这项研究的目标包括：（1）使用有限元分析和分子动力学模型对混合生物打印过程进行计算建模；（2）对不同拓扑模式、机械刺激和生化线索进行实验研究。细胞-支架相互作用；（3）响应面优化，以建立混合生物打印过程的相互作用过程参数之间的关系，以实现最佳组织工程，将在血管化水凝胶支架内研究大鼠细胞分化为成骨（骨）和软骨（软骨）谱系。生物打印参数和生物材料成分的能力将创建一种构建仿生功能梯度拓扑的有效方法，以操纵放置之间的关系。最后，再生骨软骨组织的生物化学测定和表征将为新组织再生提供见解。

项目成果

期刊论文数量（23）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Understanding Material Deformation in Nanoimprint of Gold using Molecular Dynamics Simulations

DOI：
10.3844/ajeassp.2018.837.844
发表时间：
2018-01-01
期刊：
American Journal of Engineering and Applied Science
影响因子：
0
作者：
Gaikwad, D.A. S.
通讯作者：
Gaikwad, D.A. S.

A Review of Biomedical Devices: Classification, Regulatory Guidelines, Human Factors, Software as a Medical Device, and Cybersecurity

DOI：
10.1007/s44174-023-00113-9
发表时间：
2023-08
期刊：
Biomedical Materials & Devices
影响因子：
0
作者：
Felix Tettey;Santosh Kumar Parupelli;Salil Desai
通讯作者：
Felix Tettey;Santosh Kumar Parupelli;Salil Desai

Additive Manufacturing of Compensator Devices for Radiation Therapy

放射治疗补偿装置的增材制造

DOI：
发表时间：
2020
期刊：
Proceedings of the 2020 IISE Annual Conference
影响因子：
0
作者：
Aldawood F, Desai S.
通讯作者：
Aldawood F, Desai S.

Molecular Dynamics Simulation of Poly Acrylic Acid as a Resist Material for Thermal Nanoimprint Lithography Processes

聚丙烯酸作为热纳米压印光刻工艺抗蚀剂材料的分子动力学模拟

DOI：
发表时间：
2020
期刊：
Proceedings of the Industrial Engineers Research Conference 2020
影响因子：
0
作者：
Odujole J., Desai S.
通讯作者：
Odujole J., Desai S.

Prediction of mechanical behavior of 3D bioprinted tissue-engineered scaffolds using finite element method (FEM) analysis

使用有限元法 (FEM) 分析预测 3D 生物打印组织工程支架的机械行为

DOI：
10.1016/j.addma.2020.101181
发表时间：
2020
期刊：
Additive Manufacturing
影响因子：
11
作者：
Soufivand, Anahita Ahmadi;Abolfathi, Nabiollah;Hashemi, Seyyed Ataollah;Lee, Sang Jin
通讯作者：
Lee, Sang Jin