I-Corps: Formaldehyde Scavengers as a Novel Antigen Retrieval Reagent for In Vitro Diagnostic Tests

I-Corps：甲醛清除剂作为体外诊断测试的新型抗原修复试剂

• 批准号: 1643528
• 负责人: Jason Eriksen
• 金额: $ 5万
• 依托单位: University of Houston
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1643528&HistoricalAwards=false
• 关键词: Corps; Formaldehyde; Scavengers; Novel; Antigen

The broader impact/commercial potential of this I-Corps project is the development of a commercial antigen retrieval solution that will be used in academic, government and industrial research laboratories as well as hospitals worldwide with applications for the detection of protein and molecular biomarkers. Formaldehyde is a widely used to preserve tissue in both laboratory and clinical settings. Current tissue-based diagnostic applications for the detection of biomarkers are limited due to the requirement of formaldehyde fixation of tissue samples. Due to a loss of sensitivity associated with formaldehyde fixation, many clinically relevant biomarkers cannot be reliably studied with current technology, resulting in false-negative errors. Due to the financial cost and the effect of misdiagnosis on treatment outcomes associated with false-negative errors in clinical diagnostic testing, the development of improved antigen retrieval technology will have important societal and medical impacts. Given that there are more than 93 million diagnostic tests run worldwide that depend on antigen retrieval agents, the technology is anticipated to have substantial commercial potential in clinical settings.This I-Corps project demonstrates how the detection of protein-epitopes in formalin-fixed paraffin-embedded tissue can be retrieved with higher sensitivity and reproducibility by using formaldehyde scavenging compounds. Current antigen retrieval reagents typically contain sodium citrate or Tris based solutions that rely primarily on heat to reverse formalin fixation from tissue samples. The proposed project expands on the current methods by using chemistry associated with formaldehyde scavenging to significantly enhance the detection of proteins in formaldehyde-fixed tissue samples. The proposed approach consists of novel compositions that contain combinations of formaldehyde scavenging compounds. Currently, we have demonstrated increased sensitivity with a series of structurally diverse compounds using a panel of blood vessel proteins as a model system. This method provides a new and more accurate method for the detection of proteins in tissue-based diagnostic assays used in clinical and research applications.

该I-Corps项目的更广泛的影响/商业潜力是开发商业抗原检索解决方案，该解决方案将用于学术，政府和工业研究实验室以及全球医院，并应用于检测蛋白质和分子生物标志物。甲醛是一种广泛用于在实验室和临床环境中保存组织的广泛用来。由于需要甲醛固定组织样品，因此目前基于组织的诊断应用被限制。由于与甲醛固定相关的敏感性丧失，因此无法通过当前技术可靠地研究许多临床上相关的生物标志物，从而导致错误的阴性错误。由于经济成本和误诊对临床诊断测试中错误（假阴性错误）的治疗结果的影响，改进的抗原检索技术的发展将产生重要的社会和医疗影响。 Given that there are more than 93 million diagnostic tests run worldwide that depend on antigen retrieval agents, the technology is anticipated to have substantial commercial potential in clinical settings.This I-Corps project demonstrates how the detection of protein-epitopes in formalin-fixed paraffin-embedded tissue can be retrieved with higher sensitivity and reproducibility by using formaldehyde scavenging compounds.当前的抗原检测试剂通常包含柠檬酸钠或基于TRIS的溶液，这些溶液主要依赖于热量从组织样品逆转福尔马林的固定。提出的项目通过使用与甲醛清除相关的化学作用来显着增强甲醛固定组织样品中蛋白质的检测，从而扩展了当前方法。所提出的方法由包含甲醛清除化合物组合的新颖组成组成。目前，我们已经使用一系列血管蛋白作为模型系统证明了对一系列结构上不同化合物的敏感性提高。该方法为在临床和研究应用中使用的基于组织的诊断测定法中检测蛋白质提供了一种新的，更准确的方法。