OP: Collaborative Research: Multimodal Molecular Spectroscopy and Imaging in Biological Tissue and Historical Artwork

OP：合作研究：生物组织和历史艺术品中的多模态分子光谱和成像

• 批准号: 1609983
• 负责人: Khanh Kieu
• 金额: $ 8.21万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1609983&HistoricalAwards=false
• 关键词: OP; Collaborative; Research; Multimodal; Molecular

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, the Atomic, Molecular, and Optical Experimental Physics Program in the Division of Physics, and the Office of International Science and Engineering, Professors Martin Fischer, Warren Warren, Khanh Kieu and their groups will develop optical microscopy techniques to create high-resolution images with novel molecular and structural contrast. These imaging methods can provide detailed chemical information even below the surface and do not require the physical removal of samples to create three-dimensional maps. These techniques are ideally suited for imaging of biological tissue and historical works of art, where the removal of a sample is restricted or even impossible. Information obtained from these chemical maps can help lay the scientific foundation for the development of new methods to diagnose diseases in biological tissue and give conservators information needed to repair or even prevent damage or degradation in irreplaceable works of art.The loss of function in biological tissue and historical artworks, such as discoloration or cracking in a painting or disease in tissue, generally is reflected in changes in the molecular composition or structure on a microscopic scale. A major challenge in diagnostic evaluation is that to obtain this information, mechanical sample removal is generally required. In response to this challenge, this research seeks to develop a multimodal detection method based on nonlinear pump-probe microscopy to noninvasively map pigments and their surroundings with high spatial resolution in three dimensions. Specifically, the techniques being developed access novel contrast mechanisms (such as nonlinear phase contrast) which give much higher specificity and a wider range of endogenous targets. Furthermore, critical degradation processes are being studied in tissue and artwork, including inherent (e.g. natural degradation) and induced (e.g. photodegradation) processes. Lastly, the team is working toward dissemination of suitable instruments using these techniques to the biomedical and conservation science communities.

在化学测量和成像计划中的支持下，物理学部的原子，分子和光学实验物理计划以及国际科学与工程办公室，马丁·菲舍尔教授，沃伦·沃伦·沃伦，汗·库伊（Khanh Kieu）及其小组将开发出光学微观拷贝技术，以创建光学微观的技术，以创建高质量图像和结构新颖的物质和结构形成。这些成像方法甚至可以在表面下方提供详细的化学信息，并且不需要物理去除样品即可创建三维图。这些技术非常适合对生物组织和艺术的历史作品进行成像，在这种艺术品中，将样品的去除受到限制甚至不可能。从这些化学图获得的信息可以帮助奠定科学基础，以开发新方法来诊断生物组织中的疾病，并为维修所需的维修所需的信息提供，甚至可以防止不可替代的艺术作品中的损害或降解。生物组织和历史艺术品中的功能丧失，例如在绘画或疾病中的绘画或疾病中的静止性或疾病，通常会在组织中进行变化或构图。诊断评估的一个主要挑战是要获得此信息，通常需要进行机械样品去除。为了应对这一挑战，这项研究试图开发一种基于非线性泵探针显微镜的多模式检测方法，以在三个维度中以高空间分辨率来无创绘制颜料及其周围环境。具体而言，开发的技术访问新型的对比机制（例如非线性相对比），这些机制具有更高的特异性和更广泛的内源性靶标。此外，在组织和艺术品中研究了临界降解过程，包括固有（例如自然降解）和诱导的（例如光降解）过程。最后，该团队正在努力使用这些技术传播适合生物医学和保护科学社区的合适工具。