Development of MAS NMR RF Power and Spinning Speed Controllers and 1H-X Dipolar Recoupling Methods

MAS NMR RF 功率和旋转速度控制器以及 1H-X 偶极重耦合方法的开发

• 批准号: 1608149
• 负责人: Terry Gullion
• 金额: $ 43.4万
• 依托单位: West Virginia University Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1608149&HistoricalAwards=false
• 关键词: Development; MAS; NMR; RF; Power

This project, funded by the Chemical Measurement & Imaging Program of the Chemistry Division, supports Professor Terry Gullion of West Virginia University to develop magnetic resonance methods (analogous to those used in magnetic resonance imaging) to determine the structures of proteins in the solid phase. Proteins are key components in the control of biological processes. Determination of their structures can lead to better understanding of their biochemical functions. For example, interactions of proteins on metallic nanoparticles offer potential therapies for targeting cancer cells. To help communicate this science, Dr. Gullion and his group are developing and presenting chemistry to the public, providing educational opportunities for elementary and middle school students, and developing online tutorials for undergraduate and graduate students.This work focuses on the development of magic-angle spinning nuclear magnetic resonance (MAS NMR) hardware and techniques. The first goal is the development of high-stability MAS NMR hardware necessary for long-term NMR experiments. A controller is being designed to accurately control radio-frequency (rf) power levels as needed for NMR experiments designed to determine molecular structures by dipolar recoupling. A novel method to control the sample spinning speed during MAS NMR experiments is also being developed. This enables NMR experiments that require rf pulse trains to be applied synchronously with the sample rotation. A second goal is to develop MAS NMR 1H-Ag, 1H-Au, 1H-2H dipolar recoupling experiments to determine the structures of peptides chemisorbed on gold and silver nanoparticles and to determine the relative orientation between the adsorbed peptide and the metal surface. To help undergraduate and graduate students that are just beginning to work in solid-state NMR, a series of educational tutorials describing NMR hardware is being produced and posted online.

该项目由化学部化学测量与成像项目资助，支持西弗吉尼亚大学的 Terry Gullion 教授开发磁共振方法（类似于磁共振成像中使用的方法）来确定固相蛋白质的结构。 蛋白质是控制生物过程的关键成分。 确定它们的结构可以更好地了解它们的生化功能。 例如，金属纳米颗粒上蛋白质的相互作用为靶向癌细胞提供了潜在的疗法。 为了帮助传播这门科学，Gullion 博士和他的团队正在开发并向公众展示化学，为中小学生提供教育机会，并为本科生和研究生开发在线教程。这项工作的重点是魔法的发展——角自旋核磁共振 (MAS NMR) 硬件和技术。 第一个目标是开发长期 NMR 实验所需的高稳定性 MAS NMR 硬件。 控制器正在设计用于根据核磁共振实验的需要精确控制射频 (rf) 功率水平，核磁共振实验旨在通过偶极耦合确定分子结构。 一种在 MAS NMR 实验期间控制样品旋转速度的新方法也正在开发中。 这使得需要射频脉冲序列与样品旋转同步应用的核磁共振实验成为可能。 第二个目标是开发 MAS NMR 1H-Ag、1H-Au、1H-2H 偶极重偶联实验，以确定化学吸附在金和银纳米粒子上的肽的结构，并确定吸附的肽与金属表面之间的相对方向。 为了帮助刚刚开始从事固态 NMR 工作的本科生和研究生，我们正在制作一系列描述 NMR 硬件的教育教程并发布到网上。