IDBR: Type A: Development of a yeast-based continuous culture system for detecting bioavailable phosphate

IDBR：A 型：开发基于酵母的连续培养系统，用于检测生物可利用的磷酸盐

基本信息

批准号：
1556349
负责人：
Holly Goodson
金额：
$ 43.68万
依托单位：
University of Notre Dame
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-04-01 至 2021-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1556349&HistoricalAwards=false
关键词：
IDBR Type Development yeast based

项目摘要

An award is made to The University of Notre Dame to develop a remotely deployable yeast based biosensor that measures the amount of bioavailable phosphate in water. Eutrophication, pollution of surface water supplies through excess nutrients, can result in harmful algal blooms and the degradation of the quality of surface waters throughout the globe. Thus, the Broader Impacts of the project include helping to reduce the over abundance of nutrients in water supplies, protecting the environment, drinking water, and property values, and optimizing fertilizer use (reducing farming costs and conserving the limited resource phosphate). The education and outreach efforts will include development of a simplified batch-culture-based assay for use in schools and community settings, which will broaden environmental participation and awareness of the need to reduce phosphate waste. In addition, the analytical chemistry and ecology students involved in the project will each receive cross-disciplinary training in the other field.Improving the technology for measuring phosphate is important because eutrophication (pollution through excess nutrients) is degrading the quality of surface waters throughout the globe, and monitoring of phosphate is largely still limited to laborious lab-based, wet chemistry approaches. The goal of the project is to address this problem by developing a biologically-based phosphate sensor that is continuous, relatively inexpensive, remotely deployable, able to test turbid water, and based on monitoring the growth of the yeast Saccharomyces cerevisiae. The principle behind the device is that all organisms (including yeast) need phosphate to grow; yeast are ideal organisms for this work for reasons including their robustness to environmental perturbation and resistance to water-borne viruses. Since yeast growth is linear with phosphate concentration in the relevant phosphate concentration range, changes in the amount of bioavailable phosphate in water will be measured by monitoring the growth and respiration of yeast in this continuous culture system under conditions where the test water is the only source of phosphate. Because this sensor will measure bioavailable phosphate, not simply dissolved reactive phosphate (DRP) or total phosphorous (TP) as measured by other standard approaches, it will represent an important new tool for any environmental or engineering studies involving freshwater and should provide insight into previously inaccessible aspects of nutrient dynamics in aquatic systems.

圣母大学获得了一项奖项，用于开发一种可远程部署的基于酵母的生物传感器，用于测量水中生物可利用的磷酸盐的含量。富营养化，即过量营养物质对地表水供应的污染，可能导致有害藻华和全球地表水质量下降。因此，该项目的更广泛影响包括帮助减少供水中过多的营养物质，保护环境、饮用水和财产价值，以及优化化肥使用（降低农业成本并保护有限的磷酸盐资源）。教育和推广工作将包括开发一种用于学校和社区环境的简化的基于分批培养的测定方法，这将扩大环境参与和对减少磷酸盐废物的必要性的认识。此外，参与该项目的分析化学和生态学学生将各自接受其他领域的跨学科培训。改进磷酸盐测量技术非常重要，因为富营养化（过量营养物造成的污染）正在降低整个地区地表水的质量。全球范围内，磷酸盐的监测在很大程度上仍局限于实验室的湿化学方法。该项目的目标是通过开发一种基于生物的磷酸盐传感器来解决这个问题，该传感器是连续的、相对便宜的、可远程部署的、能够测试浑浊的水，并且基于监测酿酒酵母的生长。该装置背后的原理是所有生物体（包括酵母）都需要磷酸盐才能生长；酵母是这项工作的理想生物，其原因包括其对环境扰动的鲁棒性和对水传播病毒的抵抗力。由于酵母的生长与相关磷酸盐浓度范围内的磷酸盐浓度呈线性关系，因此在以测试水为唯一来源的条件下，通过监测连续培养系统中酵母的生长和呼吸，可以测量水中生物可利用磷酸盐含量的变化磷酸盐。由于该传感器将测量生物可利用的磷酸盐，而不是像其他标准方法测量的那样简单地测量溶解的活性磷酸盐 (DRP) 或总磷 (TP)，因此它将成为任何涉及淡水的环境或工程研究的重要新工具，并且应该提供对先前研究的深入了解。水生系统营养动态中难以接近的方面。