Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish

合作研究：了解酸化和缺氧对沿海海洋鱼类代内和代际之间的影响

• 批准号: 1536165
• 负责人: Hannes Baumann
• 金额: $ 71.08万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2020-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1536165&HistoricalAwards=false
• 关键词: Collaborative; research; Understanding; effects; acidification

Coastal marine ecosystems provide a number of important services and resources for humans, and at the same time, coastal waters are subject to environmental stressors such as increases in ocean acidification and reductions in dissolved oxygen. The effects of these stressors on coastal marine organisms remain poorly understood because most research to date has examined the sensitivity of species to one factor, but not to more than one in combination. This project will determine how a model fish species, the Atlantic silverside, will respond to observed and predicted levels of dissolved carbon dioxide (CO2) and oxygen (O2). Shorter-term experiments will measure embryo and larval survival, growth, and metabolism, and determine whether parents experiencing stressful conditions produce more robust offspring. Longer-term experiments will study the consequences of ocean acidification over the entire life span by quantifying the effects of high-CO2 conditions on the ratio of males to females, lifetime growth, and reproductive investment. These studies will provide a more comprehensive view of how multiple stressors may impact populations of Atlantic silversides and potentially other important forage fish species. This collaborative project will support and train three graduate students at the University of Connecticut and the Stony Brook University (NY), two institutions that attract students from minority groups. It will also provide a variety of opportunities for undergraduates to participate in research and the public to learn about the study, through summer research projects, incorporation in the "Women in Science and Engineering" program, and interactive displays of environmental data from monitoring buoys. The two early-career investigators are committed to increasing ocean literacy and awareness of NSF-funded research through public talks and presentations. This project responds to the recognized need for multi-stressor assessments of species sensitivities to anthropogenic environmental change. It will combine environmental monitoring with advanced experimental approaches to characterize early and whole life consequences of acidification and hypoxia in the Atlantic silverside (Menidia menidia), a valued model species and important forage fish along most of the US east coast. Experiments will employ a newly constructed, computer-controlled fish rearing system to allow independent and combined manipulation of seawater pCO2 and dissolved oxygen (DO) content and the application of static and fluctuating pCO2 and DO levels that were chosen to represent contemporary and potential future scenarios in productive coastal habitats. First CO2, DO, and CO2 × DO dependent reaction norms will be quantified for fitness-relevant early life history (ELH) traits including pre- and post-hatch survival, time to hatch, post-hatch growth, by rearing offspring collected from wild adults from fertilization to 20 days post hatch (dph) using a full factorial design of 3 CO2 × 3 DO levels. Second, the effects of tidal and diel CO2 × DO fluctuations of different amplitudes on silverside ELH traits will be quantified. To address knowledge gaps regarding the CO2-sensitivity in this species, laboratory manipulations of adult spawner environments and reciprocal offspring exposure experiments will elucidate the role of transgenerational plasticity as a potential short-term mechanism to cope with changing environments. To better understand the mechanisms of fish early life CO2-sensitivity, the effects of temperature × CO2 on pre- and post-hatch metabolism will be robustly quantified. The final objective is to rear silversides from fertilization to maturity under different CO2 levels and assess potential CO2-effects on sex ratio and whole life growth and fecundity.

沿海海洋生态系统为人类提供许多重要的服务和资源，同时，沿海水域受到环境压力的影响，例如海洋酸化的增加和溶解的氧气减少。这些压力对沿海海洋生物的影响仍然很众所周知，因为迄今为止的大多数研究都检查了物种对一个因素的敏感性，但并不多于一个因素。该项目将确定模型鱼类（大西洋银层）将如何应对观察到的和预测的溶解二氧化碳（CO2）和氧气（O2）的水平。较短的实验将测量胚胎和幼虫的生存，生长和代谢，并确定父母是否经历压力状况会产生更强大的后代。长期实验将通过量化高CO2条件对男性与女性比率，终生增长和生殖投资的影响，研究整个生命周期中海洋酸化的后果。这些研究将提供更全面的观点，即多种压力源如何影响大西洋银色的种群以及潜在的其他重要饲料鱼类。这个合作项目将在康涅狄格大学和斯托尼·布鲁克大学（NY）的三个机构中支持并培训三名研究生，这些机构吸引了少数群体的学生。它还将为大学生参与研究和公众通过夏季研究项目的研究提供各种机会，并将其纳入“科学与工程领域的女性”计划中，并互动从监测浮标中展示环境数据。两位早期职业研究人员致力于通过公开谈判和演讲来提高海洋识字和对NSF资助的研究的认识。该项目响应公认的对物种对人为环境变化的敏感性的多应力评估的需求。它将将环境监测与先进的实验方法结合起来，以表征大西洋银色（Menidia Menidia），一种有价值的模型物种和美国大部分东海岸地区的重要饲料鱼类的早期和整个生命后果。实验将采用新建的计算机控制的鱼类饲养系统，以允许对海水PCO2和溶解氧（DO）含量进行独立和组合操纵，并应用静态和波动的PCO2，并选择了用于代表产品沿海栖息地中当代和潜在现状的静态和DO水平。第一二氧化碳，DO和CO2×DO的反应规范将被量化，以适应与健身的早期生活史（ELH）特征，包括既存前和既存后生存，孵化的时间，后盘后生长，从野生成人中从野生成人那里收集到从孵化后20天（DPH）收集的后代（DPH），该后代使用3 Co2×3 Co2 3 Co2 3 co2 co2 3 co2 co2 3 co2。其次，将量化潮汐和模具二氧化碳和模具的影响对银色的ELH性质的波动。为了解决有关该物种中二氧化碳敏感性的知识差距，实验室对成人产卵器环境和相互后代暴露实验的操作将阐明跨代可塑性作为应对改变环境的潜在短期机制的作用。为了更好地理解鱼类早期寿命二氧化碳敏感性的机制，将对温度×CO2对盘前和后代谢的影响进行牢固量化。最终的目标是将银色从不同的二氧化碳水平下从施肥到成熟度，并评估对性别比，整个生命生长和繁殖力的潜在二氧化碳效应。

项目成果

Are long-term growth responses to elevated pCO2 sex-specific in fish?

鱼类对 pCO2 升高的长期生长反应是否具有性别特异性？

• DOI: 10.1371/journal.pone.0235817
• 发表时间: 2020
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Murray, Christopher S.;Baumann, Hannes
• 通讯作者: Baumann, Hannes

Hypoxia and acidification in ocean ecosystems: coupled dynamics and effects on marine life

• DOI: 10.1098/rsbl.2015.0976
• 发表时间: 2016-05-01
• 期刊: BIOLOGY LETTERS
• 影响因子: 3.3
• 作者: Gobler, Christopher J.;Baumann, Hannes
• 通讯作者: Baumann, Hannes

Combined Effects of Ocean Acidification, Warming, and Hypoxia on Marine Organisms

海洋酸化、变暖和缺氧对海洋生物的综合影响

• DOI: 10.1002/loe2.10002
• 发表时间: 2016
• 期刊: Limnology and Oceanography e-Lectures
• 作者: Baumann, Hannes

Quantifying Metabolically Driven pH and Oxygen Fluctuations in US Nearshore Habitats at Diel to Interannual Time Scales

• DOI: 10.1007/s12237-017-0321-3
• 发表时间: 2018-06-01
• 期刊: ESTUARIES AND COASTS
• 影响因子: 2.7
• 作者: Baumann, Hannes;Smith, Erik M.
• 通讯作者: Smith, Erik M.

10 - Adaptation and evolutionary responses to high CO2

10 - 对高二氧化碳的适应和进化反应

• DOI: 10.1016/bs.fp.2019.07.006
• 发表时间: 2019
• 期刊: Fish physiology
• 作者: Munday, P.L.;Rummer, J.L.;Baumann, Hannes
• 通讯作者: Baumann, Hannes